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Objective: To investigate the effects of compatibility of Radix Ginseng and Radix Scutellariae (CRH) on learning and memory ability in multi-infarct dementia (MID) model rats and to explore the mechanism from the perspective of brain energy. Methods: We established MID rat model by thrombus injection, and learning and memory ability of MID rats was evaluated by Morris water maze; HE staining was used to observe the pathological changes in hippocampal CA1 region of rats; the contents of ATP, ADP and AMP in brain tissues were determined by highperformance liquid chromatography; flow cytometry was used to detect the changes of mitochondrial swelling and mitochondrial membrane potential in hippocampus of rats. Results: Compared with the rats in model group, the escape latency was significantly shortened in both high and low dose groups (P ＜ 0.05) . The times of crossing the platform were increased in both high and low dose groups (P ＜ 0.05) . The disorder of cell arrangement and loss of number of cell layers were significantly improved in high and low dose groups (P ＜ 0.05) . The degree of mitochondria swelling was significantly reduced in low dose group (P ＜ 0.05) . The decreasing trend of mitochondrial membrane potential was improved (P ＜0.05), and the energy charge of brain tissue was increased in high dose group rats (P ＜ 0.05) . Conclusion: CRH improved the cognitive decline and increased the concentration of energy charge in MID rats which was in relation to protect mitochondrial damage and improve brain energy, and further protect neurons.
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Objective To prevent phenomena of using Radix Ginseng (dry Radix Ginseng and white sugar Radix Ginseng) instead of Panax quinquefolium L. and to avoid the occurrence of side effects. Methods Panax quinquefolium L and Radix Ginseng were distinguished by their characters and fluorescence identification. Results Fluorescence color was observed by using ultraviolet lamp (254 nm) under the light-shading condition. The hadromestome of Panax quinquefolium L showed amethyst fluorescence, the dry Radix Ginseng showed blue fluorescence, and the white sugar Radix Ginseng showed bright blue fluorescence. Conclusion Fluorometric method can be used to identify Panax quinquefolinm L and Radix Ginseng, This method is simple and easy for spread.
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Objective Near-infrared (NIR) reflectance spectroscopy was used to develop a fast quality assessment method by simultaneous material identification and moisture quantification of Radix Ginseng Rubra (RGR). Methods The sample was identified by the comparison of its spectrum with a standard NIR spectral library. Similarity measurement was used as the discriminating parameter. The moisture content of sample was quantified by a partial least square (PLS) calibration model, correlative spectrum calculation was used for wavelength selection and multiplicative signal correction (MSC) was applied for pretreatment in the calibration model. Primary reference data were obtained using the traditional loss on drying (LOD) method. Results The NIR library can distinguish RGR from the counterfeit successfully. The optimized eight-factor PLS calibration model of NIR spectra has a high correlation coefficient (R=(0.999 7).) Conclusion The proposed method is rapid, accurate and can be used routinely in the traditional Chinese materia medica manufacturers for quality control of raw materials.
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Objective To establish the quality standard of Yifukang Capsule. Methods The identification of Radix Puerariae, Radix Salviae miltiorrhizae, Radix Ginseng was carried out by TLC. The content of puerarin was determined by HPLC. Results Radix Puerariae, Radix Salviae miltiorrhizae, Radix Ginseng could be identified by TLC. A good linearity of puerarin was in the range of 0.2～ 1.6 ? g(r=0.9999) and the average recovery of puerarin was 102.6 % , RSD was 1.70 % . Conclusion This method is simple, sensitive, accurate and with good reproducibility and can be used for the quality control of Yifukang Capsule.
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Objective: The similarities and the differences among Peru's Ginseng healthcare product, Radix Ginseng and Panax quinquefolium were studied. Methods: The work was performed by analysis of HPLC FPS and 4 dimensional UV FPS. Results: Their 4 dimensional UV FPS were very similar with each other, and the common peaks ratio of the components extracted from Peru's Ginseng healthcare product, Radix Ginseng with absolute alcohol was larger than that of the components extracted from Peru's Ginseng healthcare product and Panax quinquefolium. The variant ratio between the healthcare product and Radix Ginseng were more similar and much lower than that between the healthcare product and Panax quinquefolium. Conclusion: The components extracted from the healthcare product with absolute alcohol are the same as that from Radix Ginseng and are of quite large difference from the components from Panax quinquefolium.
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Objective: To establish the quality standard for Qiankun Liquid.Methods: Fructus Lycii、Fructus Cnidii、Radix Ginseng in Qiankun Liquid were identified by TLC, and the content of icariin was determined by HPLC.Results: Fructus Lycii、Fructus Cnidii、Radix Ginseng could be identified by TLC. Icariin showed a good linear relationship at a range of 0.126～1.008?g, r =0.9999. Conclusion: The method is accurate and can be used for the quality control of Qiankun Liquid.
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Objective To observe the clinical effect of Renzi Shengjing Decoction(Radix Ginseng and Fructus Schisandrae decoction for producing sperms)treating oligospermia and aspermia males with kidney yang deficiency through its influence on semen and hormones.Methods The 60 males with infertility due to oligospermia and aspermia were randomly divided into treated group and control group with 30 cases in each.Before and after treatment,the two groups were tested with the colored semen analysis system for detecting the density,motility and survival rate of sperms respectively.The follicle-stimulating hormone(FSH),luteinizing hormone(LH),testosterone(T),and prolactin(PRL)were measured by enzyme-labeling instrument.Results In the treated group,the improvement of semen parameters and sexual hormones and clinical effect were better than those of control group(P
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The thermogravimetric analysis was developed for the identification of powders and extracts of Radix Ginseng and Radix Panacis Quinquefolii. This method is simple,quick and accurate with small sample. It is avaliable for the identification of true and false product series of Radix Ginseng and Radix Panacis Quinquefolii in current market.
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A union operation method of chromatograph mass spectrometer was applied in analyses of volatile oil compositions in flowers and leaves of Radix Ginseng. The preliminary identification indicated thai there were 15 and 32 kinds of chemical compositions in flowers and leaves of Radix Ginseng, respectively. Meanwhile, their relative content has also been determined.