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Abstract@#Saposhnikovia divaricata, a perennial herb belonging to the family Umbelliferae, is widely distributed in many provinces of Mongolia. The dried root of Saposhnikovia divaricata has been used for the treatment of arthritis and as a painkiller in Mongolian folk medicine. Moreover, its dried root (Radix Saposhnikoviae) is used as a Chinese herbal medicine for the therapy of immune system, nervous system, and respiratory diseases. According to phytochemical and pharmacological studies, the main ingredients of Saposhnikovia divaricata are chromones, coumarins, acid esters, and polyacetylenes. These compounds indicate anti-inflammatory, antioxidant, analgesic, antiproliferative, and immunoregulatory activities. Cimifugin is an active ketone ingredient from Saposhnikovia divaricate, Rhizoma cimicifugae. Cimifugin has been reported to have bacteriostatic and antiviral effects. Studies have reported that cimifugin inhibits allergic inflammation by reducing the levels of cytokines. The aim of this review is to provide extensive information on the traditional use, ethnopharmacology, phytochemistry, pharmacology mechanism of action, and health products from Saposhnikovia divaricata .
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OBJECTIVE: To develop an HPLC method for determination of nine components in Huangqichifeng capsules. METHODS: The analysis was carried out on a Waters Symmetry Shield RP18 column (4.6 mm × 150 mm, 5 μm). The mobile phase was composed of methanol (A) and water (B) with gradient elution (0-15 min, 5%A → 20%A; 15-55 min, 20% A → 35%A; 55-100 min, 35% A → 85% A) and the flow rate was 1.0 mL · min-1. The detection wavelengths were set at 230 nm for paeoniflorin (0-25 min) and 254 nm for other components (25-100 min). The column temperature was 35°C. RESULTS: The linear ranges of paeoniflorin, prin-O-glucosylcimifugin, calycosin-7-0-β-Z)-glucopyranoside, cimifugin, 4'-0-β-Z)-glucopyranosyl-5-0-methylvisammi-nol, formononetin-7-0-β-Z)-glucopyranoside, sec-O-glucosylhamaudol, calycosin, formononetin were 1.510-15.1, 0.047-0.94, 2.192-10.96, 0.052 2-1.044, 0.0414-0.828, 1.56-7.80, 0.0422-0.844, 0.149-0.744 and 0.110-0.552 μg (r ≥ 0.9990) respectively. The average recoveries were 99.48%, 103.15%, 102.77%, 99.27%, 101.99%, 102.25%, 101.49%, 100.45% and 97. 49% (RSD < 2.5%, n=6) respectively. CONCLUSION: The method is simple, accurate, repeatable, andean be used to control the quality of Huangqichifeng capsules. Copyright 2012 by the Chinese Pharmaceutical Association.
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Objective: To analyze the constituents and metabolites of Radix Sa poshnikoviae (RS) in rat plasma and urine by rapid-resolution liquid chromatography-time of flight mass spectrometry (RRLC-TOF/MS), so as to explore the active ingredients and metabolites of RS in vivo. Methods: The separation was performed on a Angilent Zorbax Extend-C14 (5 μm, 250 mm x 4.6 mm id) column, with a methanol-water mobile phase system used for gradient elution. Time-of-flight mass spectrometer (TOF/MS) was applied for qualitative analysis under positive ion mode. Based on the accurate molecular weight of TOF/MS detection and the compound list of RS established previously, the constituents and metabolites of RS in different matrix in vivo were identified. Results: Six constituents of RS were identified in the plasma, sucrose, prim-O-glucosylcimifugin, cimifugin, nodakenetin, 5-O-methylvisamminol, and 3′-O-i-butyrylhammaudol. Eight constituents were identified in the urine, prim-O-glucosylcimifugin, divaricatacid, cimifugin, 4′-O-glucosyl-5-O- methylvisamminol, (3S)-2,2-dimethyl-3, 5-dihydroxy-8-hydroxymethyl-3, 4-dihydro-2H, 6H-benzo-[1, 2-b: 5, 4-b′] dipyran-6-one, 5-O-methylvisamminol, see-O-β-D-glucosylhammaudol, and wogonin. Two metabolites were identified in the urine, glucuronide of cimifujin and an isomer of it. Conclusion: The present method is reliable and effective for identifying compounds of RS in vivo, and it can provide a reference and evidence for the further pharmacodynamics experiments.
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Objective To optimize the conditions for the extraction of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules,and for the preparation of their ?-CD inclusion compounds.Methods With the percentages of extracted volatile oil of Fructus tsaoko and Radix saposhnikoviae as the target marker,single-element experiments were conducted to determine the optimum conditions for extracting the volatile oil.The application rate of included volatile oil was used as the target marker to optimize the conditions for including the oils with ?-CD.Results The optimized extraction conditions were: with 8-fold water added,the sliced herbal medicine of Fructus tsaoko and Radix saposhnikoviae should be extracted for six hours without advanced soaking with water.The optimized inclusion conditions were: applying the saturated aqueous solution method,15-fold 85 % ethanol was used to dissolve the volatile oil,the proportion of the above solution and ?-CD being 1︰8;at 25 ℃,the volatile oil was included for 30 minutes with ultrasonic wave.Conclusion The established process can be used for the extraction and the inclusion of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules.
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Objective To establish the method of fingerprint analysis on Yupingf eng Decoction,and to study the correlation of HPLC fingerprint in Radix Astraga li,Radix Saposhnikovlae,Rhizoma Atractylodis Macrocephalae and Yupingfeng Deco ction. Methods HPLC with Hypersil ODS was used,acetonitrile -water(gradient el ution) as a mobile phase and detection wavelength at 220 nm,flow rate was 1 mL ?min-1,and column temperature was 30 ℃. Results There were 9,8 and 7 common peaks separated from 10 batches of medicinal material of Radix Astragali,Radix Saposhnikovlae,and Rhizoma Atractylodis Macrocephalae,respectively;11 common peaks were separated from 10 batches of Yupingfeng Decoction,of which 6 peaks were shared by Radix Astragali,4 peaks by Radix Saposhnikoviae and 1 peak by Rh izoma Atractylodis Macrocephalae. Conclusion There exists a correlation of Yupin gfeng decoction with the medicinal pieces of Radix Astragali and Radix Saposhnik oviae. The major characteristic fingerprint peaks of Yupingfeng decoction belong to those of the isoflavones from Radix Astragali and chromones from Radix Sapos hnikoviae. This will provide a reference for the rules of the compatibility and component research of Yupingfeng Decoction.
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Objective: To study the pharmacological effects of Radix Saposhnikoviae (RL) and Fruetus Tribuli (FI) used singly and combinedly. Methods: The effect of the tested drugs on the animal itching models induced by dextran 40 and Histanmine, skin capillary permeability induced by Histamine and experimental nettle rash induced by dimethylsulfoxide (DMSO) were observed. Results: RL and FI used singly and combinedly could obviously relieve the itch, inhibit the increase of skin capillary permeability induced by Histamine and resist experimental nettle rash induced by dimethylsulfoxide (DMSO) in different degree. Conclusion:RL and FI used singly and combinedly have anti allergic effect and the effect of RL and FI used combinedly is the same as used respectively.
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AIM: To study the effects of Radix Saposhnikoviae(RL) and Feutus Trbuli(FI) used singly and combinedly on antagonizing type Ⅰ allergy,such as passive cutaneous anaphylaxis(PCA),released model from mast cell granules,Schulz-Dale respond. METHODS: Mouse and rat models mainly induced by IgE were used in experiments. RESULTS: RL and FI used singly and combinedly could significantly inhibit PCA of same species of mice,the degranulation of mast cells of skull in rats and Schulz-Dale response. CONCLUSION: Both RL and FI respectively have antagonistic effect on type Ⅰ allergy and RL in combination with FI will possess more satisactory effect
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AIM: To study the quality standard for Ganmao Qingre Granules(Herba Schizonepetae,Radix Saposhnikoviae,Radix Puerariae,etc). METHODS: Radix Saposhnikoviae,Spica Schizonepetae,Radix Bupleuri,Herba Platycodonis in Ganmao Qingre Granules were identified by TLC,and the content of puerarin was determined by HPLC. RESULTS: Radix Saposhnikoviae,Herba Schizonepetae,Radix Bupleuri,Herba Platycodonis could be identified by TLC.The concentration curve of hesperdin showed linearity over the range of 0.086 8-8.680 ?g(r=0.999 9).The average recovery was 99.09%,RSD was 1.12%(n=9). CONCLUSION: The method is simple,specific and accurate and can be used effectively for the quality control of Ganmao Qingre Granules.