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In order to study the contraindications of the compatibility of Flos Genkwa-Radix et Rhizoma Glycyrrhizae, in this study, the solubilizing and poisoning essence were explored. In this experiment, chromatographic assay, field emission scanning electron microscopy, MTT cytotoxicity evaluation, and other methods were used to study the main chemical components, morphology and toxicity of the ethyl acetate part of Flos Genkwa and its co-decoction with glycyrrhizic acid, in order to clarify Flos Genkwa-Radix et Rhizoma Glycyrrhizae incompatibility provides a new idea for the research on incompatibility of Flos Genkwa-Radix et Rhizoma Glycyrrhizae. The results showed that after co-decoction of the ethyl acetate part of Flos Genkwa with glycyrrhizic acid, high performance liquid chromatography (HPLC) detected the dissolution of the toxic component yuanhuacine of 54.8%, while yuanhuacine chromatographic peak was not detected in the Flos Genkwa ethyl acetate part of the single decoction. The increase of co-decoction dissolution rate was observed by scanning electron microscopy, and it was found that glycyrrhizic acid uniformly dispersed the fat-soluble components of Flos Genkwa into nano-scale particles, which improved the solubility and stability in the solution. Furthermore, the results of cytotoxicity evaluation showed that the survival rate of cells decreased after co-decoction, 4',6-diamidino-2-phenylindole (DAPI) staining also gave the same results. In summary, the co-decoction of the ethyl acetate part of Flos Genkwa with glycyrrhizic acid promotes the dissolution of the toxic component yuanhuacine, and makes the part form uniformly distributed nanoparticles, which is conducive to the absorption of the ingredient and increases the toxicity.
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AIM:To choose the optimal processing condition for stir-frying Radix et Rhizoma Glycyrrhizae with honey.METHODS:The contents of glycyrrhizic acid and liquiritin and macroscopical identification were selected as indexes according to orthogonal design L_9(3~4),four factors including the proportion of processed honey and water,soaking moistening time,drying temperature,and drying time.RESULTS:Optional processing conditions consisted of using two part processed honey to one part water as mixture,30 min soaring moistening time,oven temperature rose to 130 ℃ and keeping for 20 min.CONCLUSION:The above processing can make the contents of glycyrrhizic acid liquiritin maintaining more 2.33%(RSD 0.43%)and 0.9%(RSD 1.10%),the optimal honey-fried processing technology for Radix et Rhizoma Glycyrrhizae is reasonable.
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OBJECTIVE:To improve the rational compatible use of Chinese patent medicines with western medicines. METHODS:A total of 24 000 prescriptions were randomly sampled from July 10th to July 15th in 2006 for an analysis of the compatibility of Chinese patent medicines containing Salvia miltiorrhiza,Radix et Rhizoma Glycyrrhizae,Radix et Rhizoma Rhei with western medicines.RESULTS:Of the total 24 000 prescriptions analyzed,6 830(or 3 213 patients) involved combined use of Chinese patent medicines with western medicines,of which,the irrational combination for Salvia miltiorrhiza-contained,Radix et Rhizoma Glycyrrhizae-contained and Radix et Rhizoma Rhei-contained Chinese medicines with western medicines totalled 128,118 and 16 cases respectively.CONCLUSION:When the Chinese patent medicines being used in combination with western medicines,their physicochemical and pharmacological properties should be taken into fully consideration so as to achieve rational combination.
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OBJECTIVE:To establish a method for qualitative identification of Shenjin huoxue mixture.METHODS:Ly-copodii japonicum,Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the preparation were identified by TLC.RESULTS:At the corresponding positions,the color of TLC spots of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the test samples and in reference substances were identical.There was no interference form negative control.But no characteristic spots of Lycopodii japonicum in the sample were noted.CONCLUSION:The established TLC identification method of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae is reproducible,specific,reliable and suitable for the quality control of Shenjin huoxue mixture.The absence of characteristic TLC spots of Lycopodii japonicum in the sample is suggestive of the necessity of improving the production technology of Shenjin huoxue mixture.
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AIM:To choose the optimal processing condition for stir-frying Radix et Rhizoma Glycyrrhizae with honey.METHODS:The contents of glycyrrhizic acid and liquiritin and macroscopical identification were selected as indexes according to orthogonal design L9(34),four factors including the proportion of processed honey and water,soaking moistening time,drying temperature,and drying time.RESULTS:Optional processing conditions consisted of using two part processed honey to one part water as mixture,30 min soaring moistening time,oven temperature rose to 130 ?C and keeping for 20 min.CONCLUSION:The above processing can make the contents of glycyrrhizic acid liquiritin maintaining more 2.33%(RSD 0.43%) and 0.9%(RSD 1.10%),the optimal honey-fried processing technology for Radix et Rhizoma Glycyrrhizae is reasonable.
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AIM: To establish the quality standard of Agui Yangxue Tablets (Radix Angelicae sinensis, Radix Codonopsis, Rhizoma Atractylodis macrocephalae, etc.) METHODS: Radix Astragali, Radix et Rhizoma Glycyrrhizae and Radix Rehmanniae praeparata were identified by TLC. The content of ferulic acid and paeoniflorin was determined by HPLC. The HPLC condition of ferulic acid was as follows: column was Diamonsil~ TM C_ 18 (4.6 mm?200 mm,5 ?m), the mobile phase consisted of acetonitrile -0.085% phosphoric acid (22 ∶ 78), the UV detection wavelength was at 322 nm, the flow rate was 1.0 mL/min with column temperature at 35 ?C . The HPLC condition of paeoniflorin was as follows: column was Diamonsil~ TM C_ 18 (4.6 mm?200 mm,5 ?m), the mobile phase consisted of acetonitrile -0.1% phosphoric acid (15 ∶ 85), the UV detection wavelength was at 229 nm, the flow rate was 1.0 mL/min with column temperature at 25 ?C . RESULTS: The developed TLC spots were quite clear. The calibration curve of ferulic acid was linear with 0.012 9-0.154 8 ?g(r=0.999 7). The average recovery was 99.32% with RSD of 1.74% (n=9). The calibration curve of paeoniflorin was linear with 0.106 8-1.602 0 ?g(r= 0.999 9). The average recovery was 98.68% with RSD of 0.98 (n=9). CONCLUSION: The established TLC and HPLC methods are exclusive, reproducible and suitable for the quality control of Agui Yangxue Tables.
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AIM To establish the quality standard for Suzi Jiangqi Pills (Fructus Perillae,Pericarpium Citri Reticulatae,Radix Angelicae Sinensis,Radix Glycyrrhieae,etc.) METHODS TLC was performed to identify Radix angelicae sinensis and Radix et Rhizocua Glycyrrhizae.HPLC was used to determine hespiridin content. RESULTS The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The quantification method had the linearity range of 0.32-1.60 ?g.The average recovery was 98.94 % TBZ# and RSD was 1.05 % . CONCLUSION The methods of identification and quantification are simple,realizable and reproducible.It can be used effectively for the quality control of Suzi Jiangqi Pills.
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OBJECTIVE:To optimize the extraction of effective ingredients and purification for crude glycyrrhizic acid and liquiritin from Radix et Rhizoma Glycyrrhizae with different methods. METHODS:The contents and extraction percentages of glycyrrhizic acid and liquiritin were used as indexes to optimize the extraction solvent. Then the extraction process was optimized with orthogonal experiment taking the concentration of ammonium ethanol,the amount of solvent and the extracting times as indexes. Crude glycyrrhizic acid was purified with the method of recrystallization and crude liquiritin was purified with organic solvent method.RESULTS:The optimal extracting solvent was ammonium ethanol. The optimum extraction conditions were as follows:extracting the solvent 4 times(2 h/time) by adding 5 times volume of 60% ethanol(containing 0.3% ammonia water). The purity of glycyrrhizic acid was over 85% with total extraction rate at 43%,and content of liquiritin was above 15% with its total extraction rate at 67%. CONCLUSION:The method is simple and feasible and from which glycyrrhizic acid and liquiritin was been obtained,Radix et Rhizoma Glycyrrhizae can broaden the scope of the application.