ABSTRACT
Objective To know the effect ofof the particulates sampled from the air of the railway transport station of hazard chemicals on rat typeⅡpneumocytes and the antagonism of antioxidants. Methods The rat typeⅡpneumocytes cultured by routine method were pre-treated with antioxidants N-acetylcysteine (NAC), Vitamin C and Vitamin E, then were exposed to the abstract of particulates. The damages of rat typeⅡpneumocytes and DNA cross-links were detected with MTT assay and ethidium bromide fluorescence assay. Results The abstract of particulates caused obvious damages and DNA cross-links in the rat typeⅡpneumocytes and the significant dose-effect relationship was seen. NAC, Vitamin C and Vitamin E reduced significantly the damages and DNA cross-links. Conclusion Abstract of the particulates sampled from the air of the railway transport station of hazard chemicals may induce the damages and DNA cross-links in rat typeⅡpneumocytes and these adverse effects can be antagonized by antioxidants such as NAC, Vitamin C and Vitamin E.