ABSTRACT
Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
ABSTRACT
Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
ABSTRACT
OBJECTIVE: To establish the fingerprint identification and assay for the quality analysis of processed Aconitum sinomontanum Nakai from different areas. METHODS: HPLC Gradient elution method was developed to establish the fingerprints for processed Aconitum sinomontanum Nakai, and the fingerprints were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition), principal component analysis (PCA) and cluster analysis (CA). RESULTS: The common fingerprint for processed Aconitum sinomontanum Nakai fingerprints was established, and 18 common fingerprint peaks were identified. The similarity was greater than 0.90 among 10 batches of processed Aconitum sinomontanum Nakai medicinal herbs, and the contents of lappacontine and ranaconitine were determined. The samples from different areas could be classified into four groups, which reflected the quality characteristics of 10 batches of processed Aconitum sinomontanum Nakai from different areas. Four main components with cumulative contribution rate of 88.824% were selected by PCA, and seven chemical components were identified as the ones to determine the quality of processed Aconitum sinomontanum Nakai. CONCLUSION: This method, with good reproducibility and strong characteristics, can be used for the comprehensive quality evaluation of processed Aconitum sinomontanum Nakai.
ABSTRACT
Objective: To set up an analysis method of fingerprints for analgesic and anti-inflammatory effective parts from crude and processed roots of Aconitum sinomontanum (AS), and to discuss the chemical composition changes after processing that enhance the analgesic and anti-inflammatory effect. Methods: HPLC gradient elution method was developed to establish fingerprints for 10 batches of chloroform extract from crude and processed the roots of AS in different areas. And the fingerprint were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition). Results: The fingerprints of chloroform extract from crude and processed the roots of AS were set up by HPLC. The gained 3 and 15 common peaks from crude and processed roots of AS, respectively. processed product added 12 peaks, including 1 peak, 2 peak, 7 peak increase were significant, accounting for the new peak area of 72.3%-84.5%. And determination of lappacontine and ranaconitine of chloroform extract from crude and proceed products, after processing the content were reduced, ranaconitine content reduced to the original one-third. Conclusion: This method with good reproducibility, and strong characteristic, and could be used for the full quality evaluation of analgesic and anti-inflammatory effect parts from crude and processed the roots of AS. To provide scientific basis for elucidating the chemical substance base and processing principle of crude and processed roots of AS.