ABSTRACT
Abstract INTRODUCTION Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS After testing several different modifications, the adapted protocol with the best results in preliminary experiments was statistically evaluated in comparison with an automated robotic protocol. RESULTS Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). CONCLUSIONS The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.
Subject(s)
Humans , SARS-CoV-2 , COVID-19 , DNA , RNA, Viral , Sensitivity and SpecificityABSTRACT
Objective To build an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s. Methods A new rapid DNA extraction method which was suitable for traditional Chinese medicine, with equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s was verified. The filter paper strip or disk for the adsorbed nucleic acid can be directly amplified by the reaction system. Results This method can extract the nucleic acid successfully. The extracted DNA was amplified, and the results were consistent with the traditional extraction methods. Conclusion The new rapid DNA extraction method is simple, fast, and low cost, which will make nucleic acid extraction become more and more popular, and no longer confined to professional and laboratory environment. This study provides a new idea for the application and development of molecular pharmacology.