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Objective To use enzyme-linked immunosorbent assay (ELISA) for measuring thrombospondin-1 (TSP-1),and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non- idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia (BPH) and 18 patients with prostatic carcinoma.Results The TSP-1 values were (73.77±12.72)% and (121.86±-19.47)% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively (t= 8.44,P＜0.01).The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively (P＜0.01).The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.9663 and 0.7421 (P＜0.05).Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.
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Objective To explore the serological variations for syphilis in infants delivered by treated syphilitic mothers and its influencing factors. Methods Totally, 146 singleton gravidas, who had been treated for syphilis during pregrancy from January 2006 to January 2008 in our hospital, were chosen. Rapid plasma reagin(RPR) and treponema pallidum particle agglutination assay (TPPA) of these mothers before delivery and of the newborns within 3 d after delivery were tested and 92 of the 146 babies were followed up until the age of 24 months. Results (1) Among the 146 neonates, 104 (71.2%) were positive for both RPR and TPPA and 140 (95.9%) TPPA positive only. The RPR positive rate in neonates born to RPR+ + TPPA+ mothers were higher than those born to TPPA+ (only) mothers (81.4% vs 36.4%,χ2 = 25. 3, P<0. 01). 90.4% of the RPR+ neonates (94/104) showed lower or equivalent RPR titers compared to their mothers. (2) Among the 92 babies bein g followed up, the seroreversion of RPR were found in 98. 2%(n = 56) of the 57 babies, who were RPR+ +TPPA+ at delivery, at the 6 months and 100% (n=57) within 8 months, with the peak time within 2 months after birth (78. 9%, n = 45). While, 100% of the babies were found to be TPPA-within 24 mo with the peak time at 10～18 mo (64. 9%, n = 37). For those babies with TPPA+ at delivery, all turned to be TPPA- at 18 mo, with the peak time at 6 ～ 12 mo (57. 1%, n = 20). (3) The seroreversion time of babies with maternal RPR between 1:1～1:4 was later than those with maternal RPR (P<0.05). The seroreversion time of babies with maternal RPR titer of 1:4 was longer than those with maternal RPR titer of 1 > 1 [(2.5±0.8) mo vs (1. 2±0. 4) mo,P<0. 01]. However, the maternal RPR titer did not affect the TPPA reversion time (P > 0.05). The seroreversion time of RPR in infants with neonatal RPR titer of 1 : 4 was later than those with neonatal RPR titer of 1:1 [(3.7±0. 9) mo vs (2. 3±0. 6) mo,P<0. 01], and babies with RPR titer at 1 : 1 - 1 :4 showed longer duration than those with neonatal RPR- in TPPA seroreversion [(11. 2±2. 8) mo, (12.2±2.9) mo, and (11.0±2.2) mo vs ( 6. 9±2. 1) mo, P< 0.01, respectively]. Conclusions Most infants born to syphilitic mothers are serological positive for syphilis despite of standard maternal treatment during pregnancy. Infants, with higher maternal RPR titer during the pregnancy or at delivery, may persist to be serological positive for syphilis for a longer perieod, but all will turn to negative finally. Long term follow up is recommended for serological positive infants, and the diagnosis of congenital syphilis should be cautious.
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Objective To study the expression of thrombospondin(TSP) mRNA and its relationship with angiogenesis and lymph node metastasis in papillary thyroid carcinoma(PTC).Methods RT-PCT was utilized to detect the expression of TSP1 and TSP2 mRNA in 40 cases of PTC and 10 cases of normal tissue surrounding carcinoma.Immunohistochemical stain was performed to detect microvessel density(MVD) whose endothelia were marked by anti-CD105 McAb.Results The positive-expression rate of TSP1 mRNA,TSP2 mRNA was 80.0%,90.0% respectively in normal tissue surrounding carcinoma.The positive-expression rate of TSPI mRNA was 45.0%.The positive-expression rate of TSP2 mRNA was 47.5%.The MVD value of PTC cases with TSP1 and TSP2 mRNA positive expression was significantly lower than that of cases without TSP1 and TSP2 mRNA expression(P
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Objective To explore the significance of RPR te st and Tp -IgM-Capture -ELISA in fol-lowing-up of early syphilis.Methods Sixty-one cases of primary syphilis with less than one-year course and 77cases of secondary syphilis were enr olled into the study.The patients we re followed up by RPR test and Tp -IgM-Capture -ELISAat intervals of three months for two years after treatment .Results By the end of 3,6,9,12,15,18,21and 24months,RPRtest became negative in 6.2%,31.5%,61.5%,83.8%,90.7%,92.3%,94.6%,and95.3%of patients,respectively.By the end of 3,6,9,12and 15months,Tp -IgM-Capture -ELISA became negative in 25.4%,56.5%,86.2%,97.1%and 100.0%of patients,respectiv ely.In primary syphilis the RPR test became nonreactive in 8.2%(3months after treatment ),31.1%(6months),57.4%(9months),75.4%(12months),83.6%(15months),85.2%(18months),and 100.0%(21months)of patients,and Tp -IgM-Cap-ture -ELISA in 45.9%(3months),85.2%(6months),98.4%(9months),100.0%(12months)of patients.In secondary syphilis RPRtest became n onreactive in 2.6%(3months after treatment ),28.6%(6months),58.4%(9months),81.8%(12months),84.4%(15months),88.3%(18months),90.9%(21months)and 92.2%(24months)of patients,and Tp -IgM-Capture -ELISAin 9.1%(3months),33.8%(6months),71.4%(9months),94.8%(12months)and 100.0%(15months)of patients.Tp -IgM-Capture -ELISA was found to be negative earlier than that of RPR test in all ca ses.No sero-resistant was shown in T p -IgM-Capture -ELISA.Conclusions Tp -IgM turns to negative in 12months after treatment for nearly all patie nts with early syphilis.Tp -IgM-Cap-ture -ELISA may be served as a useful t ool to follow up early syphilis patie nts after treatment.[