Biophysical methods for quality evaluation of decellularized and recellularized tissue-engineered constructs of organs and tissues

Tissue engineering is rapidly growing now and can become a promising alternative to transplantation of organs and tissues,as it is devoid of major shortcomings of transplantology, such as acute shortage, complexity of selection, delivery andstorage of donor material, lifelong immunosuppressive therapy. One of the most widely known methods of obtainingbiological scaffolds for the subsequent creation of tissue-engineered constructs of organs and tissues is decellularization.The evaluation of the quality of the obtained scaffolds, based on the study of the viability of cell structures in decellularizedand recellularized matrices, is one of the priorities of modern regenerative medicine worldwide. In this investigation, thebiophysical criteria of decellularization and recellularization of tissue-engineered constructs based on the evaluation of thegeneration of free radicals in native, decellularized and recellularized tissues by EPR spectroscopy and chemoluminescencein a complex assessment of the quality of biological matrixes obtained are considered using intrathoracic organs and tissuesof rats. It has been established that the intensity indices of free radical generation in native and recellularized tissues ofanimal organs, as well as in decellularized matrices, can serve as one of the express criteria for quantitative assessment ofcell structures viability

Decellularlized Matrix in Organ Transplantation / 한양의대학술지

Liver transplantation is the only known treatment for patients with end-stage liver failure, but this therapy is limited by the shortage of donor organs. Hepatic tissue engineering combining biomaterial scaffolds and cells have been used as a promising strategy to create engineered liver graft for liver regeneration. Despite significant progress in this field, attempts to create clinically transplantable whole organs have not been as nearly successful. Recently, whole organ decellularization techniques have emerged as a new therapeutic strategy for organ replacement and provided feasibility for clinical translation. The perfusion decellularization method was applied to the whole organ for efficient removal of cellular components and generated organ scaffolds that can maintain the extracellular matrix (ECM) and vascular structure of the native organ. This review paper describes current progress in organ bioengineering for the development of transplantable liver grafts.