ABSTRACT
Background: Dysregulation of long non-coding RNAs (lncRNAs) has been found in a variety of malignancies. Previous study revealed that lncRNA-BBOX1-2 was up-regulated in gastric cancer and fibroblast growth factor receptor 1 (FGFR1) might be the potential target gene of lncRNA-BBOX1-2. Aims: To investigate the expressions and significance of lncRNA-BBOX1-2 and FGFR1 in gastric cancer. Methods: Expressions of lncRNA-BBOX1-2 and FGFR1 were detected by real-time PCR in 45 cases of gastric cancer tissues and the adjacent normal tissues. The correlations of these expressions with clinicopathological features of gastric cancer were analyzed. ROC curve analysis was used to assess the performance of lncRNA-BBOX1-2 and FGFR1 in distinguishing cancerous tissue and normal tissue and predicting lymph node metastasis. After transfected with siRNA-BBOX1-2, the expression of FGFR1 in human gastric cancer cell line SGC-7901 was detected by real-time PCR and Western blotting. Results: Both lncRNA-BBOX1-2 and FGFR1 were overexpressed in gastric cancer tissues than in adjacent normal tissues (P<0.05). The expression level of lncRNA-BBOX1-2 was positively correlated with lymph node metastasis and TNM stage (P<0.05), and the expression level of FGFR1 was positively correlated with tumor differentiation, depth of invasion, lymph node metastasis and TNM stage (P<0.05). The expression levels of lncRNA-BBOX1-2 and FGFR1 demonstrated a modest correlation with each other (r=0.344, P<0.05). When lncRNA-BBOX1-2 was knocked out by RNA interference, mRNA and protein expressions of FGFR1 were significantly decreased in SGC-7901 cells (P<0.05). ROC curve analysis showed that lncRNA-BBOX1-2 and FGFR1 might be potential biomarkers for initiation (AUC: 0.916 and 0.862, respectively) and lymph node metastasis (AUC: 0.720 and 0.774, respectively) of gastric cancer. Conclusions: LncRNA-BBOX1-2 and FGFR1 are up-regulated in gastric cancer tissues. LncRNA-BBOX1-2 might be a positive regulator of FGFR1 and their cooperation are involved in modulating tumorigenesis and development of gastric cancer.
ABSTRACT
Objective:To explore the relationship between the polymorphisms in gene FGFR1, FGF10, FGFI8 and the nonsyndromic cleft lip with or without cleft palate (NS CLP) in Chinese population. Methods: Genomic DNA was isolated from peripheral lymphocytes of 75 patients with NS CLP and their parents and 75 unimpaired healthy children. The polymorphisms in FGFRI gene rs13317, p. E467K, p. M3691 and p. S393S, FGF10 gene rs1448037 and FGFI8 gene rs4043716 were detected by applying three-dimensional (3-D) polyacrylamide gel microarray technology. The data were performed using statis-tical analysis : the genotype frequenc+ y and allele frequency between patients with NSCL/P and control subjects were performed. Haplotype relative risk (HRR) , family based association test (FBAT) , and transmission disequilibrium test (TDT) in nuclear family were performed. Results: There were no poly-morphism in FGFR1 gene p. E467K, p. M369I and p. $393S site, the corresponding base was all G. The polymorphisms of rs13317 and rs1448037 were detected and their genotype frequency and allele frequen-cy showed no significant difference between 75 patients with NSCL/P and 75 normal children. TDT, HRR and FBAT were also no significant differences. The genotype frequency of gene FGF18 rs4043716 showed significant difference, but allele frequency were no significant difference. TDT, HRR and FBAT were also no significant difference. Conclusion: Our studies suggest an association between gene FGF18 rs4043716 and the NS CLP in Chinese population, and no association among gene FGFR1 rs13317, p. FA67K, p. M3691, p. S393S and gene FGF10 rs1448037.
ABSTRACT
Objective To investigate the distribution and changes of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR-1) in normal heart or heart after myocardial infarction (MI),and to explore beneficial effects of bFGF on heart after MI. Methods Eleven adult minipigs were randomly divided into 2 groups,control (n=5) and MI group (n=6). MI was induced by ligating the left anterior descending coronary artery. Sham-operation was carried out on the animals of control. bFGF-like-immunoreactivity (bFGF-ir) and FGFR-1-like-immunoreactivity (FGFR-1-ir) in heart tissues were detected by immuno-histochemistry and image analysts in 4 weeks after surgery. Results In controls,bFGF-ir and FGFR-1-ir in the atrium showed a considerable high level compared with 2 ventricles (P