ABSTRACT
ABSTRACT Objective: To investigate the correlation between total lymphocyte and CD3+ T cell counts in peripheral blood in renal transplant patients treated with anti-thymocyte globulin, and discuss related outcomes. Methods: A single-center, retrospective study involving 226 patients submitted to kidney transplant between 2008 and 2013, and treated with anti-thymocyte globulin for induction or treatment of cellular rejection. Doses were adjusted according to CD3+ T cell or total lymphocyte counts in peripheral blood. Results: A total of 664 paired samples were analyzed. The Spearman's correlation coefficient was 0.416 (p<0.001) for all samples combined; the overall Kappa coefficient was 0.267 (p<0.001). Diagnostic parameters estimated based on total lymphocyte counts were also calculated using the number of CD3+ T cells (gold standard), with a cut off of >20 cells/mm3. Conclusion: Total lymphocyte and CD3+ T cell counts in peripheral blood are not equivalent monitoring strategies in anti-thymocyte globulin therapy.
RESUMO Objetivo: Investigar a correlação entre a contagem de linfócitos totais e células T CD3+ no sangue periférico em receptores de transplante renal submetidos a tratamento com globulina antitimocitária, e discutir resultados relacionados. Métodos: Estudo retrospectivo de centro único envolvendo 226 pacientes submetidos a transplante renal entre 2008 e 2013 e tratados com globulina antitimocitária, para fins de indução ou tratamento de rejeição celular. As doses foram ajustadas de acordo com a contagem de células T CD3+ ou linfócitos totais no sangue periférico. Resultados: No total, 664 amostras pareadas foram analisadas. O coeficiente de correlação de Spearman para as amostras em geral foi de 0,416 (p<0,001) e o coeficiente Kappa, de 0,267 (p<0,001). Os parâmetros diagnósticos estimados com base na contagem de linfócitos totais foram recalculados, empregando-se o número de células T CD3+ (padrão-ouro) e adotando-se o ponto de corte >20 células/mm3. Conclusão: A contagem de linfócitos totais no sangue periférico não substitui a contagem de células T CD3+ enquanto estratégia de monitorização da terapia à base de globulina antitimocitária.
Subject(s)
Humans , Male , Female , Adult , Kidney Transplantation , CD3 Complex , Thymocytes/immunology , Transplant Recipients , Graft Rejection/therapy , Isoantibodies/therapeutic use , Antibodies, Monoclonal/therapeutic use , T-Lymphocytes/immunology , Monitoring, Immunologic/instrumentation , Survival Analysis , Retrospective Studies , Lymphocyte Count , Flow Cytometry/methods , Immunotherapy/methods , Middle AgedABSTRACT
Objective To study the CDR3 characteristics of T cell receptor carried by auto- reactive Tcells from patients with rheumatoid arthritis. Methods BV14 and BV16 of the beta chain of TCR were ampli-fied by realtime PCR. The length of CDR3 was identified by gene scanning. The sequences of CDR3 were ana-lyzied by sequencing. Results The T cells infiltrated in the synovium of patients with rheumatoid arthritisshowed oligo clonal expansion. The beta chain carried by infiltrated T cells was encoded by different VDJ genefragments. The CDR3 had 15 bp, 21 bp and 24 bp length respectively. There were common motif among CDR3area in infiltrated T cells. Conclusion The auto- reactive T cells infiltrated in the synovium are drived byspecific antigens and therefore result restricted BV usage and common sequence motif among the T cells. Thecharacteristics of the specific antigens that drive auto- reactive T cells need further investigation.
ABSTRACT
Objective To investigate whether systemic lupus erythematosus (SLE) T cell function disorder is related to abnormal biochemical pathways.Methods After cross linking of anti CD3 mAbs to sheep anti mouse IgG and stimulating T cells,the changes of free calcium ion within T cells and these changes under interference of Thapsigargin and EGTA were observed respectively for 10 minutes with an adhesion cytometry.The relation between [Ca 2+ ]i response in SLE T cells and expression of CD3 molecules,or InsP 3 levels was evaluated.Results The base [Ca 2+ ]i response in T cells of SLE patients was similar to that of normal control ( P =0 105).Peak and plateau [Ca 2+ ]i responses were significantly higher in the group of SLE patients ( P