ABSTRACT
Objective:To investigate the roles of Th2 cytokine receptors in the pathogenesis of systemic lupus erythematosus(SLE) and its clinical significance.Methods:RT-PCR technique was used to semiquantitatively analyze Th2 cytokine receptors mRNA expression in PBMC from 89 patients with SLE,double antibody sandwich ELISA was applied to determine Th2 cytokines in serum.Results:The positive Th2 cytokine receptors mRNA expression accounted for 100% among active SLE group(group Ⅰ),inactive SLE group(group Ⅱ) and control group(group Ⅲ).The mean levels of IL-4R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.604?0.147,0.40?0.13 and 0.37?0.07,respecitively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-4R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.351).The mean levels of IL-6R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.902?0.273,0.519?0.11 and 0.573?0.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.289).The mean levels of IL-10R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.87?0.29,0.722?0.209 and 0.684?0.138,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.405).The mean levels of IL-4 in serum in group Ⅰ,Ⅱ and Ⅲ were 105.57?29.17,51.17?16.69 and 23.73?6.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL(4 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-6 in serum in group Ⅰ,Ⅱ and Ⅲ were 71.89?20.02,17.96?6.93 and 0.035?0.035,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-10 in serum in group Ⅰ,Ⅱ and Ⅲ were 32.32?11.51,18.66?6.63 and 0.289?0.867,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).There were positive correlation between IL-4 and IL-4R in active group and inactive group(r=0.622-0.859,P