ABSTRACT
Objective:To investigate apoptosis of spleen cells in infected mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg) and against challenge with Eg protoscoleces.Methods:BALB/c mice were vaccinated with the vaccine subcutaneously,intranasally,orally and intramuscularly respectively.The mice were then challenged with Eg protoscolexes at 8w of vaccination and sacrificed in 18w of infection to get spleen.Spleen cells were separated to measure apoptotic rate by FACsort with BCG and PBS served as control.Results:Apoptotic rate in the immunization group was lower than that in the control.Apoptotic rates in the oral or intramuscular group were significantly lower than that in the subcutaneous or intranasal group.Conclusion:Apoptosis of spleen cells in mice may be induced by infection with hydatid cyst,but is inhibited by immunization with rBCG-Eg95 vaccine.Oral or intramuscular vaccination may be the good regimen.
ABSTRACT
Objective To investigate the reduction of hydatid cyst weight and change of splenocyte lymphokines in mice immunized with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg). Methods BALB/C mice were subcutaneously, intranasally, orally and intramuscularly vaccinated respectively, with BCG and PBS served as controls. The mice were challenged with Eg protoscolices 8 weeks after vaccination and sacrificed in 18 weeks after infection. The weight of hydatid cyst was measured and reduction of the weight was obtained, spleens were used to separate splenocytes which were cultured under stimulation with EgAg or ConA. The supernatant was collected to measure the level of IL-2、IFN-?、TNF- and IL-4 by ELISA. Results The hydatid cyst weight reduced by 45.77%, 18.20%, 88.05% and 92.46% respectively in the 4 immunization groups. In comparison with PBS control, the level of IL-2、IFN-?and TNF-?was (30.0?0) pg/ml, (65.0?0) pg/ml and (425.0?10.7) pg/ml respectively in the intramuscular group with a significant increase, but that of IL-4 decreased, with a value of (10.0?0) pg/ml. Conclusion The recombinant BCG-Eg95 vaccination induces Th1 response in mice against challenge infection.
ABSTRACT
Objective:To dynamically observe changes of IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).Methods:BALB/C mice were intranasally or orally vaccinated and killed on 0、2、4、6、8、10、12、14、16 and 18w of immunization,with 4 in each killing.Sera were gathered from the eyeball to measure IgG, its subclass and IgE by routine ELISA,with PBS as control.Results:In the intranasal group,levels of IgG、IgG2a and IgG2b increased obviously on 2~18w,and reached the highest level on 10、4 and 6w respectively;while levels of IgG1、IgG3 and IgE decreased remarkably on 2~18w,and came to the lowest level on 8、12 and 10~12w respectively.In the oral group,levels of IgG、IgG2a and IgG2b rose remakably on 2~18w,and came to the peak value on 14、8 and 8w respectively;while levels of IgG1、IgG3 and IgE decreased on 2~18w,and reached the lowest level on 8、6 and 16~18w respectively.Conclusion:TH1 response could be induced in mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus in early immunization.