ABSTRACT
Objective To explore the application of allergen detection in children with recurrent asthma and its clinical signifi‐cance .Methods The clinical data of 524 cases of children with recurrent asthma in this hospital ,from February 2013 to February 2015 ,were retrospectively analysed .According to age ,these children with recurrent asthma were divided into three groups:250 ca‐ses were enrolled into infants group(0- <1 years old) ,150 cases enrolled into cheepers group(1- <3 years old) ,124 cases were enrolled in children group (≥3 years old) .Children in the three groups were treated with allergen detection ,and positive rates and distribution of allergens were compared among three groups .Results The total positive rate of allergen detection was 39 .69%(208/524) .The positive rate of allergen detection was the highest in children group(66 .13% ) ,and the lowest in infants group (24 .00% ) ,and there were statistically significant differences in the positive rates among the three groups(P<0 .05) .The top 3 common allergens were milk ,household dust mite and cat dander .The positive rates of household dust mite and house dust were the highest in children group ,but lowest in infants group ,there were statistically significant differences in the positive rate among the three groups(P<0 .05) .Conclusion Allergen detection is particularly important for children with recurrent asthma ,which not only could quickly find the etiology of asthma and identify children who are susceptible to asthma ,but also provide references for early intervention in childhood asthma .
ABSTRACT
Objective: To compare the curative effect of Fritillariae Cirrhosae Bulbus (FCB) in six species from five origins on recurrent asthma in mice. Methods: Eighty one female Kunming mice were randomly divided into nine groups with equal weights, such as model, control, Dexamethasone (DEX, positive control), and six FCB groups. The mice in the model, DEX, and FCB groups were sc injected with ovalbumin (OVA) on days 1, 7, and 14, then challenged with the aerosols of normal saline contained OVA on days 22-28. The mice in DEX and FCB groups were ig administered with DEX (0.6 mg/kg) and FCB (830 mg/kg) respectively for 28 d, and the mice in the control group were given 0.1% carboxymethyl cellulose. The surface tension of bronchoalveolar lavage was measured, the lung tissues were taken for hematoxylin-eosin (HE) staining, and the tracheal stenosis and volume difference of pulmonary alveolus were analyzed by the microphotograph. Results: Compared with the control group, the tracheal stenosis, surface tension, and volume difference of pulmonary alveolus were increased obviously in the model group (P < 0.01). The pathological section revealed vessel wall thickening in bronchiole, inflammatory cell infiltrating, gland hyperplasia, and mucus hypersecretion, but FCB could reverse these pathological changes (P < 0.01). Each FCB had its feature to cure asthma. F. unibracteata and F. delavayi had the most potency on increasing alveolar surfactant; F. unibracteata and F. taipaiensis had the most potency on attenuating tracheal stenosis; F. unibracteata and F. przewalskii had the most potency on relieving the inflammation; F. unibracteata and F. taipaiensis had the most potency on reducing glandular hyperplasia or intimal thickening. Each FCB had its feature to cure asthma, but F. unibracteata was the best. Conclusion: FCB has the best potency to prevent and cure the recurrent asthma in mice, but each FCB has its feature. The diversity of FCB species should be protected.