Studies on Determination Conditions for Reducing End of Hydroxyethyl Starch by DNS Spectrophotometry / 中国药师

Objective:To explore the determination conditions for reducing end of hydroxyethyl starch by DNS spectrophotometry. Methods:The reducing end of hydroxyethyl starch was determined using the standard curve of glucose solutions. The effects of DNS reagent with different volume, heating temperature, heating time and standing time after reaction on the determination were investiga-ted. Results:The optimal determination conditions were as follows:the DNS volume was 0. 8 ml, the reaction temperature was 85℃, the reaction time was 5 minutes, and the colored solution was determined at the wavelength of 540 nm. Conclusion: The method is simple and accurate with good reproducibility, which can be used to determine reducing end of hydroxyethyl starch.