ABSTRACT
The freshwater crab Potamonautes niloticus (Potamonautidae: Decapoda: Crustacea) is common in River Nile andits tributaries but has no commercial value as edible crab for human beings. Therefore, the present study aims toevaluate the role of chitosan extracted from its exoskeleton for wound healing process as a medical application. Thestructure of chitosan has been physically investigated by using fourier transformer infrared spectroscopy to reveal thevibrational frequencies of different structural functional groups, such as primary amine groups, hydroxyl groups andamide groups. The efficiency of the extracted chitosan was assessed by morphological and histological examinationof wound healing in albino rats. Sections of isolated wounds were stained with hematoxylin and eosin to evaluate thehistological changes during the wound healing process. Chitosan was yielded 30.64% from the crab shells and theresults of wound healing assessments showed that the chitosan treated group (Ch 1%) was more efficient and fasterthan other groups (Ch 2, 3%) versus control groups. Also, the total leucocyte counts showed shortened inflammatoryphase in chitosan-treated groups. The present results showed the efficiency of extracted chitosan in wound healingprocess and can be used in medical applications.
ABSTRACT
Se realizó un estudio descriptivo y transversal de 50 pacientes de ambos sexos con carcinoma espinocelular bucal, atendidos en la consulta estomatológica del Policlínico de Especialidades del Hospital Provincial Docente Clinicoquirúrgico "Saturnino Lora Torres" de Santiago de Cuba, desde septiembre del 2009 hasta igual mes del 2012, a fin de evaluar los resultados terapéuticos de la criocirugía en el tratamiento de esta enfermedad. La técnica crioquirúrgica se aplicó en la exéresis de la citada afección con repetidas congelaciones súbitas, así como descongelaciones lentas y espontáneas, razón por la cual se logró una excelente cicatrización de los tejidos dañados en el menor tiempo posible. Se demostró que la criocirugía fue un procedimiento sencillo, eficaz, fácil de aplicar, no traumática a la mucosa, sin riesgo ni complicación e inocua a los tejidos bucales; por tanto, impidió la formación de cicatrices residuales.
A descriptive and cross-sectional study was conducted in 50 patients of both sexes with oral squamous-cell carcinoma, treated at the stomatology department of the Specialties Polyclinic of "Saturnino Lora Torres" Provincial Teaching Clinical Surgical Hospital in Santiago de Cuba, from September 2009 to September 2012, in order to evaluate the therapeutic results of cryosurgery in treating this disease. Cryosurgical technique was applied in the exeresis of damaged tissues with sudden repeated freezing and spontaneous slow thawing, thus achieving an excellent healing of tissues in the possible shortest time. It was demonstrated that cryosurgery was simple, effective and easy to apply, and nontraumatic to the mucosa without risk or complication and safe for oral tissues; therefore, it prevented residual scarring.
ABSTRACT
PURPOSE: Recent studies have already demonstrated experimentally that the topical application of epidermal growth factor (EGF) accelerates the healing of partial-thickness wounds. Although previous studies have proved the effect of recombinant human EGF (rhEGF), the clinical use of rhEGF in case of patients have generated some controversy in advantage. In this study, twelve patients had partial thickness wounds because of burn and skin graft donor site were treated with rhEGF and we investigated the clinical effect of rhEGF on partial thickness wounds of patients. METHODS: We evaluated two groups of patients had partial-thickness wounds. One group (n=5) is consisted of the patients have second degree burn wounds, and the other group (n=7) is consisted of the patients have donor site wounds after skin graft. Each wound was divided two area, one area was applied twice daily with rhEGF and the other area was treated without rhEGF. The extent of reepithelized area was recorded by percentage ratio. And the statistical analysis was conducted about the difference of the number of days between two area until total wound was reepithelized. RESULTS: In the group treated with rhEGF, the mean of time to healing was about 9.75 days, whereas, in the group without rhEGF, it was about 11.08 days. In the number of days until total wound was reepithelized, there were statistically significant difference between two groups (P<0.05). CONCLUSION: It was concluded that the topical application of rhEGF accelerated the healing of partial-thickness wound clinically.
Subject(s)
Humans , Burns , Epidermal Growth Factor , Imidazoles , Nitro Compounds , Skin , Tissue Donors , TransplantsABSTRACT
Al sanar una herida se activa un proceso de reepitelización para generar una epidermis. Este proceso de cicatrización debe ocurrir rápido y efectivamente para prevenir los ataques provenientes del medio ambiente. Existe una gran variedad de cremas regeneradoras, dentro de las cuales se encuentran sucralfato (Cicalfate ®), de utilidad en medicina humana, y tartrato de ketanserin (Vulketan ®), utilizado como tratamiento convencional en medicina veterinaria. El objetivo general de este estudio fue comparar el grado de cicatrización y reacción inflamatorio entre sucralfato y tartrato de ketanserin en heridas quirúrgicas en piel del área superior de la pared torácica de conejos (Oryctologus cuniculis), los cuales se utilizaron como modelos animales para medir parámetros cicatriciales que son complicados de evaluar en forma rutinaria en estudios clínicos en el ser humano. Se emplearon 15 conejos machos. Sucralfato y tartrato de ketonserin fueron aplicados simultáneamente en 10 animales. Sucralfato se aplicó en el lado derecho y tartrato de ketanserin, en el lado izquierdo. Cinco conejos fueron tratados con suero fisiológico como grupo control. La evaluación se realizó mediante la Escala de Vancouver durante 19 días. Los resultados revelan que en el caso de ambas cremas regeneradoras no existe diferencia significativa en torno a los signos de vascularidad. Con respecto o los signos de plegabilidad, altura, pigmentación y dolor, sí existe diferencia significativa (P < 0,05) contribuyendo mejor sucralfato, por lo que se concluye que éste presento ventaja comparativa con respecto o tartrato de ketanserin al momento de realizar un tratamiento tópico para heridas en piel.
In healing o process of reepithelialization to generate epidermis start. This process must take place quickly and effectively to prevent attacks from the environment. There is a great variety of regenerative creams available such as sucralfate (Ciclalfate®) in human medicine and ketaserin tartrate (Vulketan®) used as a conventional treatment in veterinary medicine. The main objective of this study was to compare the healing degree and inflammatory reaction between sucralfate and ketanserin tartrate in surgical wounds on skin of the upper thoracic wall of rabbits (Oryctolagus cuniculis), which were used as animal models for measuring cicatricial parameters that are complicated to evaluate routinely in clinical trials in humans. Fifteen male rabbits were used Sucralfate and ketanserin tartrate were used simultaneously in 10 animals. Sucralfate was used on right side and ketanserin trartrate on the left side. Five rabbits were treated with physiological saline solution (control group). Wound evaluation were compared with the Scale of Vancouver during 19 days. The results showed that both regenerative creams had no significant differences in vascularity. In relation to pliability height, pigmentation and pain there was a significant difference (P < 0,05) between the two products in favor of sucralfate in the topical treatment of skin injuries.
Subject(s)
Animals , Rabbits , Wound Healing , Ketanserin/pharmacology , Skin , Sucralfate/pharmacology , Epithelium , Ointments , Time FactorsABSTRACT
Background: The treatment of burn injuries with Silver Sulfadiazine is used in many burn centers. Objective: Determine the duration of clinical reepithelization in children treated with enriched Silver Sulfadiazine, according to sex, age, neutral zone burned (thorax - abdomen - limbs without joint damage), extention, presence of infections, bandage adherence and derivation to rehabilitation. Method: Retrospective review of 263 clinical records during 2004 that fulfilled the items for inclusion (children age under 15 years-old, with burn injuries of partial thickness caused by scalding liquids, treated at COANIQUEM Acute Unit with Silver Sulfadiazine plus Lidocaine plus Vitamin A and not needing grafts). Normal and median position between period of clinical reepithelization and variables considered with p < 0.05 were proven. Results: The median for clinical reepithelization was 10 days (range 5 - 23); if rehabilitation was required: 15 days and not required: 9 days (p < 0.0001). Lower limbs, children under 5 years-old and girls need longer reepithelization time. 0.4%> cases presented infections and 1.1% gauze adherence. Conclusions: Duration of clinical reepithelization with enriched Silver Sulfadiazine, associated to low infection rate and few adverse effects make it highly efficient for ambulatory treatments.
Introducción: Tratamiento de quemaduras con sulfadiazina de plata, sigue siendo de uso frecuente en muchos centros. Objetivo: Determinar el tiempo de re-epitelización clínica en niños con quemaduras por líquidos calientes, tratados con sulfadiazina de plata, su variación según sexo, edad, zona neutra (tórax-abdomen, extremidades sin compromiso articular) extensión, presencia de infecciones, adherencia de aposito y derivación a rehabilitación. Metodología: Revisión retrospectiva de 263 fichas que cumplieron con requisitos de inclusión (niños < 15 años, con quemaduras causadas por líquidos calientes, de espesor parcial, ingresados al Policlínico de Agudos de COANIQUEM en 2004, tratados con sulfadiazina de plata más lidocaína y vitamina A, que no fueron injertados). Se probó normalidad y posición de medianas entre período de reepitelización y según las distintas variables, se utilizó distribución percentilar como medida de tendencia central y prueba de Kruskal Wallis para la comparación de los grupos considerando p < 0,05 significativo. Resultados: Mediana de reepitelización clínica fue de 10 días (rango 5-23) para grupo total; 15 días para los con derivación a rehabilitación y de 9 días para los que fueron dados de alta (p < 0,0001). Demoró más la reepitelización en extremidades inferiores, niños menores de 5 años y mujeres. 0,4% presentó infección y 1,1% gasa adherida. Conclusiones: Duración de reepitelización clínica con sulfadiazina de plata, sumada a la baja tasa de infección y escasos efectos adversos, aporta alta confiabilidad a este método en curaciones ambulatorias.
ABSTRACT
Recently, many works to treat chronic corneal ulcer have been progressed. It has been reported that the Aprotinin, one of them, is serine protease inhibitor and is useful to treat therapy-resistant chronic corneal ulcer because it decreases the plasmin level in tear fluid that was increased in corneal ulcer. We performed this study to evaluate the effect of Aprotinin to the reepithelization of cornea according to its concentration. We made corneal burn in rabbits and instilled topical antibiotics and Aprotinin 500u/ml and 200u/ml, four times a day. After instillation, we compared the process of corneal epithelial wound healing, according to the time interval, clinically and histopathologically in each group. As a result, wound healing of cornea treated with combination of antibiotics and Aprotinin was delayed rather than that treated with antibiotics only. And combination therapy with Aprotinin 500n/ml is more effective than with Aprotinin 2000n/ml. This data suggests that high concentration of Aprotinin alone is not helpful to tresat the therapyresistant chronic corneal ulcer.
Subject(s)
Rabbits , Anti-Bacterial Agents , Aprotinin , Burns , Cornea , Corneal Ulcer , Fibrinolysin , Serine Proteases , Wound HealingABSTRACT
The 6 eyes of 6 patients operated on using keratoconic epikeratoplasty are evaluated, and the mean 11-month postoperative results are reported. Uncorrected visual acuities improved in 4 patients and were maintained in 1 patient. One patient revealed a decrease of uncorrected visual acuity in the postoperative 6th month. Two patients showed an improvement of over 2 Snellen lines. Two patients showed the same corrected visual acuity, and that of 2 patients decreased within the postoperative 6-months follow-up. There was a significant decrease of myopia in terms of spherical equivalent. As a result, 4 cases showed a postoperative refractive error from +1.0 to -2.5D. Two cases were myopic deviated, -4.0 and -5.0D, and need further observation. In all cases, there was an effective flattening of the protruding cone with a mean decrease of over 11D of keratometry readings. Complete reepithelization occurred within 12 days, (average 7.5 days) and a moderate pressure patch was used as a routine procedure to promote the reepithelization.