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Chronic hepatitis B is a public health issue worldwide. Nucleotide analogues and interferon therapy can effectively inhibit HBV replication, but they still have the shortcomings such as inability to achieve the clearance of HBV cccDNA and low HBsAg clearance rate. The academic viewpoint of "kidney-tonifying therapy for chronic hepatitis B" provides new ideas and methods for the treatment of hepatitis B. During long-term clinical practice, Department of Hepatology in Shuguang Hospital has identified that "deficiency of spleen and kidney with damp heat remaining" is the key pathogenesis of the continuous progression of chronic hepatitis B and has established the treatment regimen for chronic hepatitis B with the basic treatment method of tonifying the kidney, strengthening the spleen, and promoting diuresis. The clinical research of National Science and Technology Major Project from The 11th Five Year Plan to The 13th Five Year Plan has validated the clinical efficacy of this regimen and clarified that regulating the immune function of the body is the main mechanism of the kidney-tonifying, spleen-strengthening, and diuresis-promoting therapy in the treatment of chronic hepatitis B.
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Chronic HBV infection is an important phase in the natural history of HBV, but there are still controversies over the treatment of this stage. Traditional Chinese medicine has had unique advantages in the prevention and treatment of viral hepatitis since ancient times and plays an important role in prevention and treatment of viral hepatitis in China. Based on the pathological process of chronic HBV infection, the team of Department of Hepatology in Shenzhen Hospital of Traditional Chinese Medicine believes that the core pathogenesis of chronic HBV infection is "kidney deficiency and epidemic toxin lurking in liver blood" and established kidney-tonifying therapy for the treatment of chronic HBV infection. Under the support of the project of Prevention and Treatment of Major Infectious Diseases such as AIDS and Viral Hepatitis in The Eleventh Five Year Plan, The Twelfth Five Year Plan, and The Thirteenth Five Year Plan, the team has conducted studies on the regularity of syndromes and a series of clinical studies and investigated the clinical efficacy of kidney-tonifying therapy through multicenter, randomized, double-blind, placebo-controlled studies, thereby exploring the application and mechanism of traditional Chinese medicine treatment in patients with chronic HBV infection. However, there are still difficulties in the pathogenesis and treatment of chronic HBV infection, and with the inheritance, innovation, and modernization of traditional Chinese medicine, it is believed that traditional Chinese medicine can provide reliable regimens for the treatment of chronic HBV infection.
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Objective:This study by using the method of network pharmacology to screen the active constituent and related targets of Epimedii Folium aims to explore the mechanisms of the reinforcing effect of Epimedii Folium on kidney in the treatment of PCOS. Methods:By retrieving data from TCMSP datebase, screened out the active constituent of Epimedii Folium and the information of the targets corresponding to each active constituent; by using the gene database of NCBI, translated the information of the targets into gene names; by retrieving data from GeneCards datebase, extracted the genes related to PCOS; related targets of Epimedii Folium in the treatment of PCOS were obtained by Venn tool; by using Cytoscape 3.7.2 software, constructed a network diagram of Epimedii Folium-active constituents-targets-PCOS; by using STRING database, constructed the protein interaction network; then carried out GO enrichment analysis of related targets by Geneontology database and carried out pathway enrichment analysis of related targets by KEGG database. Results:There were 23 active constituents of Epimedii Folium and 132 related targets treating PCOS. The Epimedii Folium could play the reinforcing effect on kidney mainly by regulating the biological processes like steroid hormone receptor activity, as well as KEGG pathways such as Estrogen signaling pathway, GnRH signaling pathway, GnRH secretion, HIF-1 signaling pathway and VEGF signaling pathway in treating PCOS. Conclusion:From the perspective of network pharmacology, this study preliminarily analyzed the related targets and pathways of reinforcing effect on kidney of Epimedii folium in the treatment of PCOS, providing reference for further experiments and application inclinics.
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"Kidney essence" is a profound concept in the theory of traditional Chinese medicine. But its biological basis is unknown until now, resulting in the therapeutic effects of traditional Chinese drugs on reinforcing kidney for supplementing essence hard to be evaluated. This study aimed, to explore the potential biological basis and mechanism of traditional Chinese drugs of reinforcing kidney for supplementing essence on diseases related to deficiency of kidney essence through network pharmacology analysis on the intersection of targets of drugs and diseases. The targets for ingredients in Rehmanniae radix praeparata (RRP), Polygoni multiflori radix praeparata (PMRP) and Polygonati rhizome (PR) were gathered from TCMSP and TCMID database. Osteoporosis, Alzheimer's disease, anemia, infertility and oligospermia targets were collected from OMIM and DisGeNET database. Drug-compound-target-disease (DCTD) network was established with Cytoscape 3.6.1 software, then Clue GO and DAVID database was used to acquire the annotation about GO terms and signaling pathways. Natural aging mice, an acknowledged syndrome model of deficiency of kidney essence, and RRP were used to verify the predictive targets by Western blot analysis. All animal experiments were conducted in accordance with the international guidelines and regulations for the care and use of animals. DCTD network showed that the intersection of drugs and diseases included 175 common targets. After topology analysis, 71 key were screened out targets which were associated with GO annotation exhibited that biological processes (including transcription regulation, RNA metabolism regulation, and DNA-dependent transcription regulation), cell composition (including nuclear lumen, organelle lumen, and membrane closure lumen), molecular function (including transcription regulation, transcription factor activity, and enzyme binding), and signaling pathway (including peroxisome proliferator-activated receptor alpha (PPARα), mitogen-activated protein kinase (MAPK), hypoxia-inducible factor 1 (HIF-1), erythropoietin (EPO) and other signaling pathways. In natural aging mice, the expressions of HIF-1α, growth factor receptor-bound protein 2 (GRB2), MAPK3, signal transducer and activator of transcription 5A (STAT5A), transcription factor AP-1 (JUN) and proto-oncogene c-Fos (FOS) in EPO pathway were significantly decreased. RRP significantly reversed the decrease of the above targets. Above all, these results indicated that the therapeutic effects of traditional Chinese drugs of reinforcing kidney for supplementing essence on deficiency of kidney essence may be related to the regulation of nuclear transcriptional activity and EPO signaling pathway.
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Polycystic ovary syndrome (PCOS) is a common gynecological reproductive endocrine and metabolic disorder disease. The clinical manifestations of PCOS are diversified, with and no ovulation, insulin resistance, hyperinsulinemia and polycystic ovaries as the main pathological and physiological changes, which can lead to non-ovulation infertility and ovulatory dysfunction infertility, cause complications, such as amenorrhea, diabetes, lipid metabolism and glucose metabolism disorder, and endanger the long-term health of the body. Traditional Chinese medicine (TCM) does not have the name of this disease, and classifies PCOS to the category of "amenorrhea", "infertility" and "abdominal mass kidney" according to its clinical manifestations. Compared with western medicine, TCM has a unique view in treating the disease, and believes that the disorders of liver, kidney and spleen functions jointly affect the occurrence and development of the disease. According to the literature review, kidney-deficiency syndrome is the main syndrome type of this disease, the kidney-tonifying method has achieved a good effect in clinic. Besides, the drug use is flexible and diversified, and both primary and secondary symptoms are taken into consideration. However, in the clinical application process, the application of kidney-tonifying method in PCOS has been affected by the thinking mode of "kidney-tonifying medicine for male diseases" to some extent. In view of this, the therapeutic effect of kidney-tonifying drugs in the treatment of PCOS is preliminarily demonstrated from several aspects of the relationship between kidney deficiency and reproduction, pathogenesis of PCOS, clinical research and experimental research of PCOS, in the expectation of providing theoretical and practical supports for kidney-tonifying therapy for PCOS and other gynecological diseases.
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Objective: To evaluate the effect of Haima Bushen Pills (HBP) on nourishing yin and reinforcing kidney, and study the mechanism of the action with aim to provide the experimental evidence for the clinic. Methods: Rats model with kidney-yang deficiency was induced by hydrocortisone through intramuscular injection to observe the anti kidney-yang deficiency effect of HBP. The effect of HBP against kidney-yin deficiency was observed using the kidney-yin deficiency model induced by oral administration of thyroxine and reserpine. The effect of HBP on erectile function was observed by intramuscular injection of hydrocortisone in rats. Castrated male rats and normal young male rats were administered orally to observe the androgenic effect of HBP. Results: After 10 d of administration, HBP at dose of 1.1 g/kg and 2.2 g/kg significantly increased the number of autonomic activities of kidney-yang deficiency mice, extended the duration of low-temperature swimming, and increased the organ coefficients of thymus and spleen. It also significantly reduced the number of platform jumping errors and prolonged the incubation period of errors, significantly increased the pain threshold and increased the organ coefficients of thymus and spleen in mice with kidney-yin deficiency. After 2 weeks of administration, HBP at doses of 0.56 g/kg and 1.12 g/kg significantly increased the bR/rR ratio and intravitreal pressure in rats, significantly improved the erectile function, significantly increased the serum testosterone (T) concentration and the viscera coefficients of prostate, foreskin gland and levator ANI of castrated rats. The serum concentration of testosterone (T), follicle-stimulating hormone (FSH) and viscera index of seminal vesicle were significantly increased in normal young rats after administration of HBP. Conclusion: HBP has obvious effect on nourishing yin and reinforcing kidney.
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[Objective]The article summarizes the experience of professor ZHOU Enchao in treating Chronic Kidney Disease.[Methods]The article discuses the clinical experience of professor ZHOU Enchao in the treatment on Chronic Kidney Disease from the methods of learning from his daily treatment in inpatient and outpatient department,reading relative books and articles,recognition from the study of ancient classics,from etiology and pathogenesis,treatment,drug characteristics,illustrating Professor's medication experience in the treatment of Chronic Kidney Disease and one case.[Results]Professor ZHOU Enchao believes that the disease is caused by kidney deficiency,mixed with wet,Fengxie and blood stasis.Pro.ZHOU Enchao treats Chronic Kidney Disease by Reinforcing Kidney,Strengthening Spleen,Promoting Qi,pairing drugs.And these usually lead to good curative effect.[Conclusion]Professor Zhou's clinical experience in treating Chronic Kidney Disease from yishen is well worth extending in clinic for it always has significant effect.
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Kidney deficiency and blood stasis is the main cause of ovulatory dysfunctional infertility. Kidney deficiency is the main pathological mechanism. Blood stasis is the main pathological manifestation, and it is an important factor throughout. Bushen Culuan Chongji is under the guidance of traditional Chinese medicine(TCM) theory, previous years of clinical experience, combined with the etiology and pathogenesis of anovulatory infertility and modern pharmacological research results, selected, not only maintains the TCM syndrome differentiation and different diseases features, but also reflects the superiority of the combination of disease. In the study of Bushen Culuan Chongji in the treatment of anovulatory infertility, there was no acute toxicity and no LD50 was detected. No adverse effects and side effects were found in the reproductive, genetic, toxicity, teratogenic, and perinatal tests in their high and low dose groups. In pharmacodynamics experiments, it can promote the development and maturation of follicles and the formation of corpus luteum in rats. Taking the kidney deficiency and blood stasis syndrome as the breakthrough point, systematically study the efficacy, safety and mechanism of six ovulatory dysfunctional infertility diseases, including abnormal uterine bleeding-ovulatory disorders, polycystic ovary syndrome, high prolactin, luteinized unruptured follicle syndrome, luteal phase defect and diminished ovarian reserve/premature ovarian failure. It verified the contribution degree of reinforcing kidney and resolving stasis TCM, reflected the characteristics of combination between disease differentiation and syndrome differentiation, interpreted the treatment principles of treating different diseases with the same method, and provided scientific basis for clinical treatment.
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Objective To investigate the clinical efficacy of mind-regulating and kidney-reinforcing acupuncture in treating attention deficit hyperactivity disorder (ADHD).Methods Fifty-six patients with attention deficit hyperactivity disorder were randomly allocated to treatment and control groups, 28 cases each. The treatment group received mind-regulating and kidney-reinforcing acupuncture and the control group, conventional acupuncture, for a total of 28 days. The American Connell children’s ADHD behavior diagnostic scale and the Chinese version of ADHD SNAP-IV scale-parent form were used as evaluating indicators. The total efficacy rates were compared between the two groups of patients.Results The total efficacy rate was 96.4% in the treatment group and 71.4% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in the American Connell children’s ADHD behavior diagnostic scale score and the Chinese version of ADHD SNAP-IV scale-parent form score in the two groups (P<0.05). There were statistically significant post-treatment differences in the American Connell children’s ADHD behavior diagnostic scale score and the Chinese version of ADHD SNAP-IV scale-parent form score between the treatment and control groups (P<0.05). Conclusion Mind-regulating and kidney-reinforcing acupuncture is an effective way to treat attention deficit hyperactivity disorder.
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OBJECTIVE:To systematically review the efficacy and safety of reinforcing kidney and invigorating spleen princi-ple in the treatment of primary osteoporosis (POP) in middle and elderly aged patients,and provide evidence-based reference for clinic. METHODS:Retrieved from VIP,Wanfang Database,CJFD,Medline,PubMed,Elsevier,Springer,Web of Science and Ovid,randomized control trials (RCT) about reinforcing kidney and invigorating spleen principle (test group) versus other meth-ods(no reinforcing kidney and invigorating spleen principle,control group)in the treatment of POP were collected. Meta-analysis was performed by using Rev Man 5.3 software provided by Cochrane after data extraction and quality evaluation modified by Jadad scale. RESULTS:Totally 28 RCTs were enrolled,involving 2849 patients. Results of Meta-analysis showed,the total effective rate [OR=3.80,95%CI(2.76,5.23),P<0.001],BGP level [MD=2.23,95%CI(1.80,2.66),P<0.001],BMD level of femoral neck [MD=0.04,95%CI(0.00,0.08),P=0.05] and BMD level of lumbar spine [MD=0.07,95%CI(0.03,0.11),P=0.001] in test group were significantly higher than control group,ALP level [MD=-5.59,95%CI(-10.51,-0.66),P=0.03] and VAS score [MD=-1.38,95%CI(-1.87,-0.89),P<0.001] were significantly lower than control group,with statistical significance. There were no significant differences in U-Ca/Cr [MD=-0.07,95%CI(-0.17,0.02),P=0.12] and serum Ca2+ level [MD=0.16,95%CI (-0.07,0.39),P=0.17] in 2 groups. CONCLUSIONS:Reinforcing kidney and invigorating spleen principle can improve patients' osteogenesis during bone metabolism,reduce the activity of the osteoclastic process and relief patients'pain in the treatment of POP.
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Objective To evaluate the efficacy of reinforcing kidney and removing stasis combined with thalidomide in the treatment of multiple myeloma.Methods 120 patients with multiple myeloma from January 2011 to January 2015 in department of traditional Chinese medicine of the third hospital affiliated to Liaoning medical university were divided into two groups.The control group received VAD program ( vincristine, doxorubicin and dexamethasone), and the observation group received reinforcing kidney and removing stasis combined with thalidomide on the basis of control group.All patients received treatment for four weeks of one course.Results After treatment, the total efficiency of observation group was higher than that of control group(75% vs.60%, P<0.05).Compared with control group, the M protein,plasmocyte count and osteoclasts count of observation group were lower, the osteoblast count of observation group was higher(P<0.05).The improvement rate of quality of life (KPS score) in observation group was higher than that in control group (70.00% vs.51.67%,P<0.05).The changes of renal function (serum creatinine, urea nitrogen) and adverse reaction in observation group were less than those in control group (P<0.05).Conclusion The reinforcing kidney and removing stasis combined with thalidomide has the significant effect in the treatment of multiple myeloma with fewer adverse reactions and higher quality of life after treatment.
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?AlM: To explore the effects of the prescription of reinforcing kidney, nourishing blood, improving eyesight on the oxidative stress model of ARPE-19 cells induced by acrolein. ?METHODS:SD rats serum containing the prescription of reinforcing kidney, nourishing blood, improving eyesight and the content of distilled water in serum were prepared. The effects of the prescription and distilled water in serum at different concentration ( 2. 5%, 5%, 10%, 20% and 40%) on cell vitality was observed by cell counting kit ( CCK-8 ) assay. the logarithmic phase of ARPE-19 cells were pretreated by different concentrations (1. 25%, 2. 5%and 5%) of the prescription serum and distilled water in serum for 24h. Then it was treated with 75μmol/L acrolein for 24h. Cell vitality was observed by CCK-8 assay. The change of cell nucleus was detected by DAPl staining . ?RESULTS: 2. 5% and 5% serum had no effect on cell viability (P>0. 05), while 10%, 20%, 40% serum could inhibit cell viability (P ?CONCLUSlON: The prescription of reinforcing kidney, nourishing blood, improving eyesight has the protective effect on ARPE-19 cell damage induced by acrolein.
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By observing the arthroscopy cleaning technique with postoperative oral administration with reinforcing kidney and activating blood Chinese herbal medicine in the treatment of knee osteoarthritis (KOA), this article was aimed to evaluate the clinical curative effect and its influence on serum C endⅡ collagen type (CTX-Ⅱ) and cartilage oligomeric matrix protein (COMP). Patients who met the diagnostic criteria were randomly divided into the treatment group and control group, with 30 cases in each group. Joint limited debridement was firstly applied. And the degree of articular cartilage degeneration, synovial hyperplasia and inflammatory degree were observed and recorded. Preoperative venous blood was collected. The contents of CTX-Ⅱ and COMP were detected with ELISA method. After surgery, in the treatment group, surgery plus postoperative oral administration with reinforcing kidney and activating blood Chinese herbal medicine was given. In the control group, surgery plus oral administration with celecoxib capsules was given. Four weeks before and after the treatment, the WOMAC knee function score was used in the evaluation of clinical curative effect. The contents of postoperative serum CTX-Ⅱ and COMP were determined. The results showed that the effectiveness of the treatment group was higher than that of the control group (P < 0.05). In the treatment group, the WOMAC score in pain, rigidity, difficulty of activity and total score had significant differences compared with the control group (P < 0.05). There were statistical significances on serum CTX-Ⅱ and COMP in the treatment group before and after the treatment, as well as the comparison between the treatment and control group both before and after the treatment (P < 0.05). Serum CTX-Ⅱ and COMP had positive correlation with the degeneration of cartilage and synovial hyperplasia with statistical significances (P < 0.05). It was concluded that joint limited debridement under arthroscopy combined with reinforcing kidney and activating blood method had synergistic effect in the treatment of KOA. It can effectively relieve the clinical symptoms and improve the knee joint function. It can also obviously reduce the contents of serum CTX-Ⅱ and COMP. There was internal close relation between the articular cartilage degeneration, synovial hyperplasia under the arthroscopy in KOA and cytokines of CTX-Ⅱ and COMP. The cytokines of CTX-Ⅱ and COMP provided important references for the estimation of articular cartilage degeneration and synovial hyperplasia.
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Objective To explore the mechanisln of action of Jianpiyishen Decotion on renal fibrosis and provide experimental evidence for chnieal application.Methods The Wistar male rats were randomly divided into four groups.They were normal control group(N group),model group(M group),low dose treatment group(L group)and high dose treatment group(H group).AⅡanimal models were made of CRF with subtotal renal ablation except the N group.Interference began at one week after operation.After being interfered for 8 weeks,blood serunl and nephridial tissues were taken out.Serum urea nitrogen(BUN)and creatinine(Scr)and fibronectin(FN)were detected.Results In N group。There were light expression in renal tubniointerstitial substance,cellula epithelialis basal meulbnule and Vascular smooth muscle.In M group,FN was strong expressed in renal tubulointerstitial substance and renal tubde,and also in glomerular mesangium.There was significant difference between N and M group(P<O.01).FN was medium expressed in renal tubuloinlerstitial substanceand renal tubule in L and H group.The expression of FN in L group was higher than that of N group(P<0.01)and lower than that of Mgroup(P<O.01).The expression of FL in H group was lower than that in L group(P<O.05).Condusion Jianpiyishen decotion can decrease eerum BUN and Cr ofCRF rats,restrain the expression of FN in kidney tissue.And it indicates that Jianpiyishen decotion is an effective herbal prescription for the treatment of CRF.The mechanism may be because it can restrain the expression of the cell factor such as FN in rats'kidney,and delay the progress of renal fibrosis and CRF.
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Objective To study the clinical efficacy of Reinforcing Kidney Decoction for the treatment of metabolic syndrome diagnosed as traditional Chinese medicine (TCM) syndrome of phlegm-blood stasis due to kidney deficiency. Method 42 recipients who volunteered to accept the TCM therapy were up to the inclusion criteria and finally fulfilled this study. Of all the cases, there were 26 males and 16 females, their old years ranged from 39 to 60, which were divided into obesity type diabetics group and metabolic syndrome group, both of which were treated with oral Reinforcing Kidney Decoction combined with western medicine therapy, with a course of 4 weeks. The changes of clinical symptoms, body weight index (BMI), fasting blood glucose (FBG), 2 h post prandial glucose (2hFBG), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL-C) levels, and serum uric acid were analyzed. Results Therapeutic effect in two groups ranked as significant, general and null effect, 14/5/2 vs 8/11/2, respectively. Dizziness and heaviness feeling of limbs have been improved. Rate of syndrome scores and laboratory parameters descended great significant, Hotelling's Trace, F =16.51, P =0.00. Compared with pretreatment, the levels of TC, TG, SUA obviously descended (P
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[Objective] To evaluate the significance of plasma endothelin in tubal pregnancy (TP) and to observe the regulatory effect of kidney-reinforcing, blood-activating and blood-stasis-removing Chinese herbal medicine on endothelin (ET) level. [Methods] Plasma ET level and ET-positive rate were detected in 40 cases of TP, 20 of intrauterinc pregnancy (IP) and 20 of chronic pelvic inflammation (CPF) and the relationship between the two above indexes in the patients were analyzed. TP patients were randomized to three groups; group A (n = 15) was treated with No. 1 Yishen Huoxue Pills (6g, tid) and Penyankang mixture (25mL, tid) for two treatment courses; group B (n = 15) was treated with aspirin (257ng, qd) for two treatment courses; group C ( n - 10) was not given any medicines. [Results] ET-positive rate and plasma ET level in postoperative TP patients were higher than those in IP patients ( P .05) . Chinese herbal medicine could decrease ET-positive rate and plasma ET level in postoperative TP patients (P
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[ Objective ] To observe the therapeutic effect of Bushen Huoxie Decoction (mainly composed of Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Dioscoreae, Carapax et Plastrum Testudinis, Radix Polygoni Multiflori, Fructus Lycii, Radix Morindae Officinalis, Herba Cistanches, Rhizoma Acori Tatarinowii, Radix Polygalae, etc. ) for the treatment of vascular dementia (VD) . [Methods] Eighty cases of VD were randomized into two groups by using random number table: group A ( n = 40) was treated with modified Bushen Huoxie Decoction and group B (n = 40) with duxil and nimodipine. After a 3-month treatment, the therapeutic effect of the two groups was evaluated, and mini-mental state examination (MMSE), activities of daily life (ADL) and blood rheology before and after treatment were observed. [Results] The total therapeutic effective rate was 80.0% in group A and 57.5% in group B, the difference being significant ( P
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Objective To explore the effect of Chinese herbal medicine formula "tonifying kidney plus strengthening vital energy" on regulating CD40, CD40L protein and mRNA expression in spleen cell in mice with SHENXU. Methods The interaction of CD40L expressed by activated T cells with CD40 on monocytes, macrophages, or B cells is essential for the production of IL-12 and other cytokines. An experimental model of mice with SHENXU by corticosterone was established to observe the effect of Chinese formula on CD40, CD40L protein and mRNA expression in spleen cells or in ConA stimulated spleen cells by immunohistochemistry and RT-PCR. Results No significant differences were found among 5 groups of mice about the CD40 and CD40L protein expression in spleen cells. Both CD40 and CD40L expression detected by immunohistochemistry decreased seriously in ConA induced cells from SHENXU mice (18.9%?3.3% vs 15.8%?2.3%) as compared to that of normal controls(29.7%?4.2% vs 23.3%?1.3%), and so did the mRNA expression on ConA stimulated cells from SHENXU mice. They were restored in different degrees when the SHENXU mice were cured by different Chinese medicine. The effect of "tonifying kidney plus strengthening vital energy" was stronger than that of "tonifying kidney" and "strengthening vital energy" alone. Conclusions "Tonifying kidney plus strengthening vital energy" enhanced the expression of CD40, CD40L mRNA and protein, and this may be one of the mechanisms of improving the status of immune inhibition resulted by Corticosterone.
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[Objective] To observe the anti - aging effects and explore the mechanism of Qibao Meiran Pellet (QMP) and its modified prescriptions, including QMP with qi - invigorating and spleen - strengthening herbs, with kidney -nourishing herbs or with blood - activating and purgative herbs. [ Methods ] Ten - month - old model rats were established by D - galactose. Serum free radical levels and immune indexes in QMP group and various modified QMP groups were observed. [ Results ] QMP increased the activity of superoxide dismutase ( SOD). QMP combined with kidney - nourishing herbs or blood - activating and purgative herbs decreased the serum level of malondialdehyde (MDA) and increased the activities of glutathione peroxidase (GSH - Px) and SOD. Meanwhile, QMP combined with kidney -nourishing herbs increased interleukin - 2 activities. [ ConclusionJ QMP, mainly composed of herbs for nourishing liver and spleen, can delay the aging of rats, and spleen - strengthening herbs and blood - activating and purgative herbs also play an important role in the delay of aging. Furthermore, it is suggested that the anti - aging effect of herbal medicine is related to the sex.
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To observe the effect of kidney reinforcing and blood nourishing herbal medicines in treating anovulatory sterility (AS) and its mechanism. Two hundred and seventy six cases of AS with hypopituitarism confirmed by the examination of blood sexual hormones were divided into three groups to receive the treatment of promoting ovulation. Group A was treated with modified Zhenwu Decoction and Reinforcing Qi and Nourishing Yin Powder (one dose per day) from the third day of menstrual cycle (MC) to the ovulating day; Group B with clomiphene (50mg/d) from the fifth day of MC, with human chorionic gonadotrophin (HCG) added, 10?000?U im in the day of follicle maturity and Group C with human menopausal gonadotrophin (HMG) 25 U/d im from the eighth day of MC and then with HCG added, 10 000 U im in the day of follicle maturity. After treatment, the cases were asked to have sexual intercourse for three nights. Ovulation time was monitored by ultrasound B imaging for one time every other day until follicle became maturity (1 8?cm?1 8?cm).Early pregnancy was detected by HCG test.Ninety three MC were ovulatory among 167 MC in Group A, the ovulation rate being 55 68%; 182 among 230 in Group B , the rate being 79 13% and 88 among 134 in Group C , the rate being 65 67%. Multisample ? 2 test showed that the ovulation rate in Group A was lower than that in Group B and Group C (? 2=9 345, P