ABSTRACT
ABSTRACT Equigan is an anabolic steroid that has been developed for veterinary use and derived from endogenous sex hormone testosterone that plays a key role in the development of male reproductive tissue as well as in puberty and spermatogenesis. The current study is aimed to investigate the possible prophylactic effect of star anise extracts (SAE) on the toxicity of rat testes, sexual hormones alternations, sperm count, sperm abnormalities and testicular DNA damage by Equigan. Forty adult male rats were equally divided into four groups (1st Control group, 2nd SAE group, 3rd Equigan and 4th Equigan+SAE group). Food and fluid intakes, relative body weight, potassium, chloride, phosphorous, non-progressive and immotile sperms were significantly increased in Equigan group as compared to control group. In contrast; relative testes weight, sodium, magnesium, total calcium, testosterone, FSH, LH, PRL, sperm count, progressive motility, and viability showed a significant decrease in Equigan group as compared to control groups. The relative weight of epididymis, seminal vesicles, prostates and serum calcium ions didn't change significantly in different studied groups. Co-administration of SAE with Equigan improved the sexual toxicity, electrolyte alternations, sperm count, abnormalities and DNA damage induced by Equigan.
Subject(s)
Animals , Male , Rats , Plant Extracts/analysis , Reproductive Techniques , Illicium/adverse effects , Reproductive Physiological Phenomena , Spermatogenesis/drug effects , Bodily Secretions , DNA Fragmentation/drug effects , Fertility Agents, Male/analysis , Anabolic Agents/pharmacologyABSTRACT
RESUMEN Con el objeto de caracterizar su manejo y desempeño productivo y reproductivo, se aplicó una encuesta en 66 Sistemas de Producción Ovinos de Trópico Alto (SPOTA) en Colombia. La recopilación de la información se llevó a cabo a través de una encuesta experta compuesta por 32 variables (10 productivas y 22 reproductivas). Los indicadores estadísticos se calcularon a través del programa SAS® v.9.1. Los Spota se distribuyeron en tres categorías en función del objetivo de producción: carne y lana (59%), genética y carne (24%) y carne (17%). El control de peso de los animales se realizó con báscula de reloj y el método de esquila fue manual en estos sistemas productivos. Las variables productivas edad al destete y peso al beneficio presentaron menores valores en los SPOTA de carne. Se observó la implementación de programas de monta libre continua (en 82% de los sistemas de tipo carne y lana) y de monta controlada continua (en 81% de los sistemas de tipo genética y carne, y en 73% en las de carne). Las variables reproductivas días abiertos y vida útil de los machos presentaron mayores valores en los Spota de carne y lana. Se encuentra que el manejo y desempeño productivo y reproductivo de los Spota presenta un mejor comportamiento en los sistemas ovinos de los tipos genética y carne y carne. En los sistemas ovinos de carne y lana no se realiza un adecuado control productivo y reproductivo que permita sostener la oferta de productos en el mercado.
ABSTRACT ER_Sixty-six sheep farms in High Tropic (HT) of Colombia were surveyed about management and productive and reproductive performance. Information collection was conducted by an expert survey composed of 32 variables (10 productive and 22 reproductive). For this purpose sheep systems were stratified according to production purpose, averages, statistics indicator were calculated through SAS® v.9.1. Farms were distributed in meat and wool systems HT (59%), genetics and meat HT (24%) and meat HT (17%). Sheep HT systems performed weight control through scale clock and shearing method was manual. Weaning age and slaughter weight shows lower values in meat systems. Continuous free rides (82% in meat and wool) and continuous controlled rides (81% in genetics and meat y 73% in meat) was observed. Higher values in days open and life in males was observed in sheep meat and wool systems. From this study we were able to conclude that management and productive and reproductive performance, presents a better behavior in meat and genetics and meat ovine production systems HT. In meat and wool systems dont exists an adequate productive and reproductive control to sustain market offering.
ABSTRACT
Introducción: dentro de las técnicas de reproducción asistida de baja tecnología está la inseminación intrauterina, donde se colocan los espermatozoides dentro del útero de una mujer cerca del momento de la ovulación. Presentación del caso: se presenta el caso de una paciente de 31 años de edad, color de piel blanca, de procedencia rural (municipio Minas de Matahambre, Pinar del Río, Cuba) con el único antecedente personal de infertilidad secundaria y obstétrico de gesta 1, parto 0, aborto 1 a los 18 años, con DIU T de cobre por nueve años para lo cual fue atendida junto a su pareja en la consulta de infertilidad del policlínico universitario "Hermanos Cruz" de Pinar del Río, donde se le diagnosticó trastornos de ovulación e infección vaginal y una oligoastenospermia con varicocele izquierdo a su pareja, para lo cual llevaron tratamiento médico quirúrgico combinado. Conclusión: se logró primero la varicocelectomía y espermatogénesis con merapur (HMG), la ovulación con HMG y clomifeno, luego se realizó una fertilización de baja tecnología, con lo que se logró un embarazo, que llegó a feliz término a las 41 semanas de gestación. Por lo riesgoso de este tipo de embarazo y el resultado feliz que tuvo, se decidió publicar el caso.
Introduction: among assisted reproduction techniques of low technology is intrauterine insemination, where sperm is placed in the uterus of a woman near the time of ovulation. Case report: the case of a female 31-year-old patient, white skin, of rural origin (Minas de Matahambre Municipality, Pinar del Rio, Cuba) presents for the sole personal history of secondary infertility and obstetric geostation 1, delivery 0, abortion 1 to 18, with Copper T IUD for nine years for which she was served with her partner in the office of infertility at Hermanos Cruz university polyclinic of Pinar del Rio, where she was diagnosed disorders ovulation and vaginal infection and her partner with a left varicocele oligoasthenospermia, which led to combined surgical treatment. Conclusion: varicocelectomy was first achieved and spermatogenesis with merapur (HMG), ovulation with HMG and clomiphene, and then a low-tech fertilization took place, bringing a pregnancy, which came to fruition at 41 weeks of gestation was achieved. So this kind of risky pregnancy and was happy result, it was decided to publish the case.
ABSTRACT
The ultrastructure of the reproductive gland, dorsal body (DB), of Megalobulimus abbreviatus was analysed. Electron microscope immunohistochemistry was used to detect FMRFamide-like peptides in the nerve endings within this gland. Nerve backfilling was used in an attempt to identify the neurons involved in this innervation. In M. abbreviatus, the DB has a uniform appearance throughout their supraesophageal and subesophageal portions. Dorsal body cells have several features in common with steroid-secreting gland cells, such as the presence of many lipid droplets, numerous mitochondria with tubular cristae and a developed smooth endoplasmic reticulum cisternae. Throughout the DB in M. abbreviatus numerous axonal endings were seen to be in contact with the DB cells exhibiting a synaptic-like structure. The axon terminals contained numerous electron-dense and scanty electron-lucid vesicles. In addition, the DB nerve endings exhibited FMRFamide immunoreactive vesicles. Injection of neural tracer into the DB yielded retrograde labelling of neurons in the metacerebrum lobe of the cerebral ganglia and in the parietal ganglia of the subesophageal ganglia complex. The possibility that some of these retrograde-labelled neurons might be FMRFamide-like neurons that may represent a neural control to the DB in M. abbreviatus is discussed.
Foi analisada a ultraestrutura da glândula reprodutiva corpo dorsal (CD) de Megalobulimus abbreviatus. Imunoistoquímica para microscopia eletrônica foi utilizada para detectar peptídeos relacionados ao tetrapeptídeo FMRFamida nas terminações axonais existentes nessa glândula. Foi utilizada marcação neuronal retrógada com o intuito de localizar os neurônios envolvidos nesta inervação. O CD de M. abbreviatus possui um aspecto uniforme em toda sua extensão, tanto na porção supraesofágica como subesofágica. As células do CD possuem várias características de glândulas esteroidogênicas, tais como a presença de inúmeras gotículas lipídicas, numerosas mitocôndrias com cristas tubulares e cisternas bem desenvolvidas de retículo endoplasmático liso. Por toda a extensão do CD de M. abbreviatus foram encontradas numerosas terminações axonais fazendo contatos estruturalmente semelhantes a sinapses com as células do CD. As terminações axonais continham grande número de vesículas eletrodensas e esparsas vesículas eletrolúcidas. As terminações axonais no CD apresentavam vesículas com conteúdo imunorreativo à FMRFamida. A injeção de traçador neural no CD resultou em marcação retrógrada de neurônios no metacérebro dos gânglios cerebrais e nos gânglios parietais do complexo ganglionar subesofágico de M. abbreviatus. É discutida a possibilidade de que estes neurônios identificados por marcação retrógrada possam representar a via de controle neural do CD de M. abbreviatus, cujo mediador químico seria um neuropeptídeo relacionado à FMRFamida.