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Braz. j. microbiol ; 39(2): 307-310, Apr.-June 2008. ilus
Article in English | LILACS | ID: lil-487709

ABSTRACT

The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.


O mecanismo de resistência bacteriana ao mercúrio mais comum é baseada na redução do Hg(II) a Hg0, através da atividade da enzima mercúrio redutase (MerA). O uso do fragmento de 431 pb amplificado de uma região conservada do gene merA, que codifica a enzima MerA,foi utilizado como marcador molecular deste mecanismo, permitindo a identificação de bactérias resistentes ao mercúrio.


Subject(s)
DNA Repair Enzymes , Environmental Microbiology , In Vitro Techniques , Mercury/analysis , Oxidoreductases/analysis , Genetic Markers , Methods
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