ABSTRACT
Background: Clinical studies, reliable geospatial data, and blood bank management all require an understanding of blood group trends. The prevalence of ABO in the blood group varies from one community to another. Every transfusion center/hematology lab is required to keep a statistical record of the blood group among their population, staff, and students. Aim and Objectives: Determining ABO and Rh blood group and study the pattern of these blood groups with an estimation of gene frequencies among first phase medical students of GMC Jammu. Materials and Methods: 250 medical students were recruited for the study. The finger-prick technique was done to obtain blood. On clean glass slides, a drop of monoclonal anti-A, B, and D was added to a drop of red blood cell suspension made from finger-prick blood and normal saline and thoroughly mixed. The agglutination results were subsequently recorded. Percentages were used to represent the data. Results: ABO blood group prevalent in this study was B, which accounted for 39.6% of all cases (36.8% B+ and 2.8% B-) followed by O with 34% (33.2% O+ and 0.8% O-), A with 21.2% (20% A+ and 1.2% A-), and AB with 5.2% (5.2% AB+ and 0% AB-). Among Rh group 95.2% were positive whereas 4.8% were found to be negative. The gene frequency for IA (p) - 0.1599, for IB (q) - 0.2571, and IO (r) - 0.5830. Conclusion: The B blood group is more ubiquitous than the others, with the AB blood group being the least common. Rh-positive is more common than Rh-negative blood types. Gender had no effect on the ABO and Rh blood groups.
ABSTRACT
Objective: Blood transfusions are commonly used in themanagement of patients with chronic renal failure (CRF)undergoing hemodialysis for dialysis-related anemia.Accordingly, they are intended to alloimmunization due torepeated transfusions. Therefore, the purpose of this study wasdesigned to identify the distribution patterns of ABO bloodgroup and Rh-D, C, c, E, e antigen to provide first step donordata bank to prepare red blood cell panel to minimize rhesusalloimmunization among the hemodialysis patients and blooddonors at renal dialysis center, Khartoum and Wad Madani,Sudan from December 2016 to December 2017.Methods: Samples from 351 blood donors and recipients wereserologically tested for ABO, Rh (D, C, E, c, and e) using slideand tube method. ABO and Rh Phenotypes' frequencies werecalculated as a percentage.Results: The ABO blood grouping showing a typical frequencyO >A>B>AB and also the frequency of rhesus antigen showingD >c>e>C>E in both male and female renal failure patients.Conclusion: Blood donors need special precautions tominimize any possible alloimmunization by such antigen.
ABSTRACT
BACKGROUND: To ensure safety of blood transfusion, accuracy in performance of blood grouping tests (BGT) is essential. External proficiency testing (PT) for BGT has not been conducted in Korea. The first PT for BGT in domestic blood centers was conducted in order to evaluate the domestic status of accuracy of BGT in blood centers and to aid in improving the quality of blood centers. METHODS: Whole blood survey specimens consisting of three panels for ABO grouping and two panels for Rh typing were sent to 81 blood centers. Evaluation criteria for BGT were as follows: 'Good' for answers with 100% referee consensus, 'Acceptable' for correct answers other than those of the referee, and 'Unacceptable' for answers other than those of 'Good+acceptable' as correct answers. RESULTS: Rates of correct answers on three panels for ABO grouping were all 100%; however, that of cell typing for the panel with BW was 61.7%, and 31 blood centers incorrectly reported normal 'B' type as an answer. The rate of correct answers for the Rh negative panel was 100%; however, that for the weak D panel was 84%, and 13 blood centers incorrectly reported Rh negative type as an answer. CONCLUSION: Findings from this study demonstrated that some hospital blood centers were not able to correctly detect blood groups with weak antigens. Therefore, to improve the quality of blood centers, intensive education for blood center staff and continued PT for BGT should be required.