ABSTRACT
Objective: To distinguish Rhizoma curcuma of different origins using headspace-gas chromatography/mass spectrometry (HS-GC/MS) combined with principal components analysis (PCA) and hierarchical cluster analysis (HCA), so as to help the quality control of Rhzzoma curcuma. Methods: HP-5 capillary column (30 m×0. 32 mm, 0. 25 μm) was used under the following condition; inlet temperature; 250°C, initial column temperature; 50°C maintained for 3 min, then increasing to 150°C at 20°C/min and to 200°C at 2°C/min, maintained for 10 min, with the split ratio being 10 : 1. The carrier gas was helium, with flow rate being 1. 0 mL/min, had space vials regional temperature being 90°C, vials heating equilibration time being 30 min, and injection volume being 1. 5 mL. The effect of extract separation conditions, temperature of the vial and equilibrium time on the extraction volatile components of RMzoma curcuma were observed. Results: PCA could distinguish 18 common peaks of 15 batches of Rhizoma curcuma from Sichuan, Guangxi and Yunnan, and it was confirmed that (3-elemene, camphene, 13-pinene, p-menth-l-en-8-ol, eucalyptol, and cycloisolongifolene, 8, 9-dehydro-9-formyl were the main components to cause differences in RMzoma curcuma of different origins. Conclusion: We have established a method combining HS-GC/MS with PCA and HCA to distinguish RMzoma curcuma of different origins, and we have also identified the major characteristic components of Rhizoma curcuma of different origins.
ABSTRACT
OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285?g/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma.