ABSTRACT
Objective To study the amount of rhizospheric microorganisms and soil enzyme activity influenced by Paridis Rhizoma in different locations and of different strains. Methods The amount of rhizospheric microorganisms, soil enzyme activity and their correlation were researched through field survey and collection of rhizospheric soil in Paridis Rhizoma cultivated in Three Gorges Reservoir Region and by microbial dilution plate culture method. Results The amount of rhizospheric microorganisms in Paridis Rhizoma from different habitats showed significant differences. The dominant species in soil microflora was bacteria; the second one was actinomycetes; the fewest one was fungus. The variation trend of the amount of rhizospheric microorganisms was not consistent with the variation trend of rhizospheric microorganisms diversity index. The activity of soil phosphatase, invertase and pepsin in Paridis Rhizoma from different habitats varied. The correlation analysis showed that the correlation between the soil enzyme activity and the amount of rhizospheric microorganisms existed. Conclusion Choosing the suitable strains and habitats of Paridis Rhizoma is beneficial to enhancing the amount of rhizospheric microorganisms and soil enzyme activity, which can create good micro-ecological environment for growth and cultivation of Paridis Rhizoma.
ABSTRACT
Objective: To characterize rhizospheric microbial flora of Hunyuan Astragali Mongolici Radix (HAMR) and to provide scientific evidence for understanding quality formation of the herb. Methods: The soil microbial flora were fingerprinted by automated ribosomal intergenic-spacer analysis (ARISA). The raw data were processed and analyzed by GeneMarker and PAST, respectively. Results: Compared with non-rhizospheric soil flora, the rhizospheric flora from 2-and 3-year-old herbs shifted obviously, and the similarities were decreased but diversities increased. Meanwhile, the flora from 5-year-old herbs had no significant similarity difference to non-rhizospheric ones and their sample rare fraction curves were more consistent with each other. When compared to the ones from other production areas, the flora from HAMR had relatively lower core OTUs but had significantly higher abundance of core OTUs shared by all areas. Conclusion: The structure of rhizospheric microbial flora of HAMR is associated with the herb's age and is quite different from thoes from other areas.