ABSTRACT
OBJECTIVE: To study the bacteriostatic action of polysaccharides of the root and stem of Rosa laevigata.METHODS: The bacteriostatic action of the polysaccharides of the root and stem of R.laevigata was determined by punching method in agar diffusion method.Four holes were punched in every medium and marked by No.1,2,3 and 4.A few drops of culture solution of bacteria or fungi were dripped to culture medium to be evenly distributed using smear bar.Hole No.1 was added with antibiotics,hole No.2 blank control,No.3 polysaccharides solution of the root of R.laevigata and No.4 polysaccharides solution of stem of R.laevigata at different concentrations;then the medium was put into incubator for culture of a period of time in a suitable constant temperature. Then the bacteriostatic circle diameter was observed.RESULTS: Polysaccharides of the root and stem of R.laevigata had inhibitory action on staphylococcus albus,staphylococcus citreus,staphylococcus aureus,standard klebsiella pneumoniae and dysentery bacillus in a dose-dependent manner.CONCLUSION: The polysaccharide of both the root and the stem of R.laevigata have bacteriostatic action;and the root and the stem of R.laevigata have no significant differences in bacteriostatic action.