Serologic and molecular characterization of rotavirus from children with acute gastroenteritis in Chennai, Tamil Nadu, India

Background: Rotavirus gastroenteritis is the leading cause of diarrhea in infants and young children worldwide. Although, Rotavirus vaccine has been introduced in 2017 in states like Tamil Nadu, there are reports of the role of Rotavirus as one of high disease burden agents with genetic variants arising, especially from low-income countries like India.Methods: Authors evaluated stool samples from 507 children with acute gastroenteritis Rotavirus A among the hospitalized children (>5 years) to provide baseline information on changing profile in this state. The stool samples were collected and screened for Rotaviral Antigen by Enzyme Immuno Assay and use of semi-multiplex RT PCR technique was conceded out in order to conclude the P and G genotypes of human rotavirus in rotavirus-positive samples from January 2014 to December 2016 in and around Chennai, India.Results: Of 507 samples collected 213 (42.01%) were positive for rotavirus antigen by Enzyme Immuno Assay (EIA). The maximum positivity (75%) was in the age group of one to two years. Rotavirus positives were subjected to further VP7 and VP4 molecular characterization and the predominant genotypes identified were G9P[4] followed by G9P[8], G1P[8], G3P[8], G2P[4] and mixed types of G2G9 with P[4] and G4P[6][11] with few untypable strains.Conclusions: This study had demonstrated the Rota Virus Gastro Enteritis (RVGE) is a common disease affecting the pediatric population and G9P[4], G9P[8] circulating types among the gastroenteritis cases reported in the city and its suburban area. This study in comparison to previous ones shows that the dominant serotypes and circulating genotypes changes from time to time within country. The results have reemphasized the need of rotavirus vaccines with broad serotype coverage which may help in decreasing the disease burden in this region of the country.

Effectiveness Of Structured Teaching Programme On Knowledge Regarding Prevention And Management Of Rotavirus Infection Among Mothers Of Under-Five Children In Selected Rural Areas

Back ground of the study: Rotavirus is a virus that infects the bowels. It is the most common cause of severe diarrhea among infants and children throughout the world and causes the death of about 6,00,000 children worldwide annually. The name rotavirus comes from the characteristic wheel-like appearance of the virus when viewed by electron microscopy (the name rotavirus is derived from the Latin Rota, meaning "wheel").Material and methods:”one group pre-test, post-test design” was selected.The sample comprised of 100 mothers of under five children living in selected rural areas at keerapakkam village. Technique was used inPurposive sampling technique. Result: The mean pretest knowledge score was 15.62, post test knowledge score was 29.62 at 0.05 level of significance. Standard deviation of pretest is 3.03, post test is 1.95 which indicates that the STP was effective in increasing knowledge of mothers of under-five children regarding prevention and management of rotavirus infection.

Hepatitis por Rotavirus: A propósito de un caso / Rotavirus hepatitis: About a case

Introducción: La infección por rota virus se ha reconocido como un patógeno en el humano y causante de enfermedad diarreica grave; la infección se ha asociado con meningitis aséptica, enterocolitis necrotizante, miositis aguda , abscesos hepáticos, neumonía, enfermedad de Kawasaki , síndrome de muerte súbita y enfermedad de Crohn. En este caso se informa la asociación con hepatitis infecciosa. Caso Clínico: Masculino, con colitis alérgica a las proteínas de la leche de vaca y de la soya, desde el nacimiento. Al mes y medio presenta enfermedad diarreica por rota virus asociada a hepatitis. Conclusiones: Tradicionalmente se ha considerado que es una infección del intestino delgado y que todos los eventos fisiopatologicos son el resultado de este tipo de lesión y de la intolerancia alimentaria que provoca a nivel local, sin embargo se han informado enfermedades que se presentan en otros órganos, como respuesta a la infección viral del sistema digestivo. En esta ocasión la invasión por el virus provoca daño hepático.

We describe a 3- month-old infant that was diagnosed with hepatitis secondary to infection by the rotavirus. The rotavirus has been recognized as a human pathogen that has been associated with severe diarrhea, aseptic meningitis, necrotizing enterocolitis, acute miositis, hepatic abscesses, pneumonia, Kawasaki disease, sudden infant death syndrome, and inflammatory bowel disease such as Crohn’s. To our knowledge, only three reports have associated the rotavirus with acute hepatitis, however, one of the three reports was in an immunodeficient patient. In this report we present another case of acute hepatitis secondary to the rotavirus, in an immunocompetent patient.

Rota virus G1P8 isolated from acute diarrhea children with rhesus kidney cells

Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.

Epidemiological surveillance of rota virus diarrhoea in Vietnam during the period from August 1998 to December 2002

Epidemiological surveillance of rota virus diarrhoea in Vietnam during the period from August 1998 to December 2002

Prevalence and Identification of Rotaviruses in Stool Specimens of Patients with Acute Diarrhea from Several Regions of Korea

PURPOSES: Rotaviruses are the main cause of infantile diarrheal diseases worldwide. The purpose of this study is to obtain epidemiologic data of rotavirus infections in Korea. METHODS: Stool specimens were collected from 150 patients with acute diarrheal symptoms, who were admitted to Yonsei Medical Center and Chungbuk National University Hospital. After isolating the virus from the specimens, the viruses were identified as rotaviruses by electron microscope and fluorescent microscope after staining with rotavirus VP6-specific monoclonal antibody. RNA was extracted from the specimens by modified phenol/chloroform method. Electropherotying was done with extracted RNA samples after silver staining of the gels. Rotavirus serotyping was done using commercial serotyping ELISA kit. RESULTS: Rate of infection from rotavirus stool samples was 60%. Isolated rotaviruses were all serogroup A and a majority(46.0%) of these viruses were long type. Serotypes 1 and 2 were identified with serotype 1 being the majority(52.2%). There were no differences in these types between 1991 and 1992, and also between Seoul and Cheongju area. CONCLUSION: Electropherotype of rotavirus epidemic in Korea was serogroup A, and long type in the majority. Serotypes of rotavirus epidemic in Korea were type 1 and type 2. These results show that the rotavirus reassortant vaccine developed in America could also handle the rotaviral disease in Korea.