ABSTRACT
Objective To study the hemoprotective effects of a rotary magnetic field (RMF) to radiation-injured mice. Methods 132 male BALB/c mice were randomly divided into four groups: a normal group (N), a magnetic treatment group (M), an irradiation group(R) and an irradiation combining magnetic treatment group (R + M). Mice in the N group received no treatment. Mice in the R and R + M groups received total body irradiation with 6.0 Gy 60Co γ/rays. Mice in the M and R + M groups were treated with a RMF for one and half an hour at a time, twice a day, totally for 30 days. The survival rate was observed for 30 days. On days 0, 5, 9, 15, 21, 30, the subjects' peripheral blood cells were counted. On day 9, 23 and 30, the number of bone marrow nucleated cells (BMNCs), colony forming unit-spleen (CFU-S), spleen-body ratio, the cell cycle and apoptosis of bone marrow cells were measured. The pathological sectioning of the femur was performed and the expression level of bone morphogenetic proteins (BMP2/4) in the bone marrow was evaluated. Results ①No mice died in the N and M group. The RMF treatment increased the survival rate and survival days among the irradiated mice (P < 0.01). ②The RMF treatment increased the number of blood cells in their peripheral blood of the R + M group. ③The number of BMNCs, CFU-S and the proportation of G2 + M stage in the R + M were markedly higher than that of the R group, but the proportation of the apoptosis was lower than that of the R group on the 9th day (P < 0.05). Furthermore, the spleen index in the R + M group was also higher than that of the R group on the 23rd day (P < 0.05). ④RMF could improve the expression level of BMP2/4 in the radiation-injued mice. Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation and it accelerated the recovery of hematopeiesis and the hematopoietic microenvironment in mouse bone marrow.
ABSTRACT
Objective:In Order to study on biological effects of rotary magnetic field (RMF) conveniently and precisely,a digital-controlled instrument for generating RMF,which is based on a single-chip microcomputer,is developed.Methods:The precise frequency of RMF is controlled by using microcomputer to drive step motor,and intensity of magnetic field is measured by using 10-bit Analog-to-Digital(A/D) Converter module which is integrated in microcomputer.According,the technique of LCD screen to display relative information,such as,frequency,intensity of magnetic field,time of timer,is applied.Additionally, touching buttons,which is simple and convenience to use,is selected.Results:This instrument works very well with high precise frequency,and its operation is simple.As a example for this instrument,that biological effect of RMF on the the prostate about SD rat is significantly is found.Conclusion:This instrument may be used to research and study on biological effects of RMF widely.
ABSTRACT
Objective To study the hemoprotective effects of a rotary magnetic field(RMF)with radiation- injured mice.Methods C57 BL6/J mice were randomly divided into a control group and a magnetic treatment group.The mice received total body irradiation with 7.0 Gy and 6.5 Gy ~(137)Cs?rays.The treatment group was trea- ted with a RMF for one hour at a time,twice a day.The intensity of the RMF was 0.6T.The survival rate was ob- served for 30 days.On day 7,10,14,21,28 after irradiation,the subjects' peripheral blood cells were counted.On day 12 and 16,the number of bone marrow mononuelear cells(BMNCs)was measured and their ability to form granu- locyte-macruphage colony-forming unit(CFU-GM)was assessed.The pathological sectioning of the femur was per- formed and the expression level of bone morphogenetic proteins(BMPs)in the bone marrow were evaluated.Re- sults The RMF treatment increased the survival rate and duration among the irradiated mice and the number of blood cells in their peripheral blood.Also,RMF treatment could increase the number of BMNCs and improve their ability to form CFU-GM on days 12-16.Furthermore,RMF could improve angiogenesis and the expression level of BMPs. Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation,and it accelerated the recovery of hematopoiesis and the hematopoietic microenviroment in mouse bone marrow.
ABSTRACT
Objective To observe the effects of rotary magnetic to bone mineral content(BMC), bone specific alkaline phosphatase (BAP) and deoxypyridinoline crosslinks (DPD), and to explore if there is synergistic action between the rotary magnetic field and the Chinese Medicine. Methods Experimental rats were exposed to the magnetic field 2 hours a day for 15 days then measured BMC, BAP and DPD then compared with that of the control rats. Results After rotary magnetic treatment, both female and male rats demonstrated a significant increase in BMC and BAP, and a simultaneous decrease in DPD content. Addition of Chinese medicine did not cause any significant difference of these parameters. Conclusion In rats with ovariotomy, rotary magnetic field can increase BAP and decrease DPD in a short time,and increase bone mineral content. These effects were similar in both female and male rats.