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Spathopsis rubens is freshwater bivalves distributed in the Nile River and its main canals all over Egypt.Four populations of S. rubens on Al-Mahmoudia irrigation canal at Damnhour, Egypt were collected andinvestigated for morphometric characters and genetic diversity by using inter simple sequence repeats (ISSR).The results declared that both the length and height measurements of the collected samples from the differentlocations showed slight significant differences; however, no significant differences between the total weightand the width of the samples have found. High degree of correlation between temperature and total weight,height and length was reported in samples collected from the third location. Genomic DNA from the selectedsamples of each population was extracted and amplified using 10 ISSR primers. The primers (M2, M3, M8,M12, M17, F2, F4, and F9) showed 100% polymorphism. Unweighted pair group method with arithmetic meandendrogram characterized the samples of S. rubens into two main definite clusters. The cluster of genotypes2 and 3 recorded the highest similarity and distance indices at a distance of 0.60859, while genotypes 1 and 3recorded the lowest similarity and distance indices at a distance of 0.1716.
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The study is planned to find the antimicrobial activity of the extract of Jania rubens and to isolate the bioactive compound against MRSA and VRSA. Jania rubens collected from Mandapam (Pudumadam) Coastal water, East coast of India and extracted with ethanol. Antibacterial activity of J. rubens was tested against gram positive, gram negative bacteria and drug resistant bacteria). The antibacterial activities were expressed as zone of inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) Identification of compounds from crude extract of J. rubens carried by column chromatography, thin layer chromatography and NMR analysis. Finally J. rubens could serve as useful source of new antibacterial agent.
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El estudio de las pinturas barrocas, especialmente las de origen flamenco, permiten evidenciar en algunas de sus figuras signos físicos sugestivos de enfermedad. Peter Paul Rubens constituye uno de los máximos representantes de esta corriente artística. Se cree que Rubens padeció artritis reumatoidea (AR) y que retrató el desarrollo y progresión de su propia enfermedad en varias obras de arte. Este artículo relata brevemente la vida de Rubens y describe algunos de sus trabajos centrados en la figura humana con alteraciones articulares que podrían corresponder a una AR
The study of baroque paintings, especially those of Flemish origin, makes it possible to show in some of their figures physical signs suggestive of disease. Peter Paul Rubens constitutes one of the maximum representatives of this artistic current. It is believed that Rubens suffered from rheumatoid arthritis (RA) and that he portrayed the development and progression of his own disease in several works of art. This article briefly recounts Rubens' life and describes some of his works focused on the human figure with joint alterations that could correspond to an AR
Subject(s)
Paintings , Art , Arthritis, RheumatoidABSTRACT
Objective To explore the in vivo anticancer, anti-angiogenesis and immunomodulatory efficacies of the bioactive polysaccharide isolated from cold aqueous extract of Jania rubens (JCEM) and Pterocladia capillacea (PCEM) as well as hot aqueous extract of Enteromorpha intestinalis (EHEM) against hepatocellular carcinoma rat model (HCC) and to study their chemical composition. Methods The sugars and amino acids composition of the bioactive polysaccharides of JCEM, PCEM and EHEM were determined using gas liquid chromatography and amino acid analyzer, respectively. These polysaccharide extracts (20 mg/kg b.wt. for 5 weeks) were assessed on hepatocarcinogenesis in rats and α-fetoprotein (AFP), carcinoembryonic antigen (CEA), glypican-3 (GPC-3), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) and Ig G levels were evaluated. Results The GLC analysis of JCEM, PCEM and EHEM polysaccharide revealed the presence of 10, 9 and 10 sugars, in addition the amino acid analyzer enable identification of 16, 15 and 15 amino acids, respectively. These polysaccharide extracts of JCEM, PCEM and EHEM produced significant decrease in serum AFP, CEA, GPC-3, HGF and VEGF compared with untreated HCC group. JCEM, PCEM and EHEM had an immunostimulatory responses by increasing the IgG levels as compared by naïve value (1.23, 1.53 and 1.17 folds), respectively. The bioactive polysaccharides in HCC induced rats improved the humoral immune response. The photomicrographs of liver tissue sections of the groups of HCC treated with polysaccharide extracts of Jania rubens and Enteromorpha intestinalis showed intact histological structure. Moreover, fractions HE1, HE4, HE7 obtained from polysaccharide of EHEM showed moderate cytotoxic activity against HepG2 in vitro with IC
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OBJECTIVE@#To explore the in vivo anticancer, anti-angiogenesis and immunomodulatory efficacies of the bioactive polysaccharide isolated from cold aqueous extract of Jania rubens (JCEM) and Pterocladia capillacea (PCEM) as well as hot aqueous extract of Enteromorpha intestinalis (EHEM) against hepatocellular carcinoma rat model (HCC) and to study their chemical composition.@*METHODS@#The sugars and amino acids composition of the bioactive polysaccharides of JCEM, PCEM and EHEM were determined using gas liquid chromatography and amino acid analyzer, respectively. These polysaccharide extracts (20 mg/kg b.wt. for 5 weeks) were assessed on hepatocarcinogenesis in rats and α-fetoprotein (AFP), carcinoembryonic antigen (CEA), glypican-3 (GPC-3), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) and Ig G levels were evaluated.@*RESULTS@#The GLC analysis of JCEM, PCEM and EHEM polysaccharide revealed the presence of 10, 9 and 10 sugars, in addition the amino acid analyzer enable identification of 16, 15 and 15 amino acids, respectively. These polysaccharide extracts of JCEM, PCEM and EHEM produced significant decrease in serum AFP, CEA, GPC-3, HGF and VEGF compared with untreated HCC group. JCEM, PCEM and EHEM had an immunostimulatory responses by increasing the IgG levels as compared by naïve value (1.23, 1.53 and 1.17 folds), respectively. The bioactive polysaccharides in HCC induced rats improved the humoral immune response. The photomicrographs of liver tissue sections of the groups of HCC treated with polysaccharide extracts of Jania rubens and Enteromorpha intestinalis showed intact histological structure. Moreover, fractions HE1, HE4, HE7 obtained from polysaccharide of EHEM showed moderate cytotoxic activity against HepG2 in vitro with IC 73.1, 42.6, 76.2 μg/mL. However, fractions of PCEM and JCEM show no or weak cytotoxicity against HepG2 in vitro where the cytotoxic activity of their crude polysaccharide extract proved synergetic effect.@*CONCLUSIONS@#The pronounced antitumor activity of sulfated polysaccharide-protein complexes of JCEM and EHEM is due to direct cytotoxic activity, anti-hepatocarcinogenesis, and anti-angiogenesis. In addition, JCEM, PCEM and EHEM had an immunostimulatory response and improved the humoral immune response in HCC induced rats.
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O presente artigo aborda a importância das obras de Antonin Artaud e de Rubens Correa para que Nise da Silveira pudesse articular duas proposições no campo da saúde mental: uma sobre a organização dos serviços de saúde mental e outra acerca da possibilidade de uma transformação cultural. A primeira proposição diz respeito ao corte epistemológico em relação ao modelo médico, possibilitado pela introdução do conceito de inumeráveis estados do ser, de Antonin Artaud. A segunda proposição se dá a partir das apresentações teatrais desenvolvidas pelo ator Rubens Correa, encenando temas relativos à loucura.
The following article deals with the importance of Antonin Artaud's and Rubens Correa's works so that Nise da Silveira could undertake a double modification in the field of mental health: a change in the organization of mental health services and a cultural transformation. The first change takes place through an epistemological cut in relation to a medical pattern, made possible by the introduction of the concept of countless states of (human) being, by Antonin Artaud. The second change takes place from the theatrical presentations developed by Rubens Correa, showing themes related to madness.
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Tendo como tema o assunto abordado no primeiro artigo publicado pela Revista da AMRIGS, comenta-se a evolução havida na terapêutica clínica de uma situação bastante comum na prática do internista e do cardiologista e presta-se homenagem aos cinqüenta anos desta publicação e ao insigne professor Rubens Maciel (1913-2004) (AU)
Focusing on the theme discussed in the first article published by the AMRIGS journal, this is an account of the progress made in the clinical therapeutics of a very common situation in the practice of internists and cardiologists, as well as a tribute both to the 50 year anniversary of this journal and to the honorable Professor Rubens Maciel (1913 2004) (AU)