ABSTRACT
Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL-1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.
Subject(s)
Humans , Adolescent , Ascomycota/metabolism , Rumex/metabolism , Rumex/microbiology , Endophytes/metabolism , Phytochemicals/metabolism , Ascomycota/isolation & purification , Ascomycota/growth & development , Time Factors , Coculture Techniques , Rumex/growth & development , Seedlings/growth & development , Seedlings/metabolism , Seedlings/microbiology , Endophytes/isolation & purification , Endophytes/growth & developmentABSTRACT
Objective To study the chemical constituents from the root of Rumex gmelini.MethodsThe compounds were isolated and purified by chromatography,polyamide column chromatography,and preparation HPLC etc.Their structures were elucidated by physicochemical and spectroscopic evidences.Results Ten compounds were identified as:nepodin (Ⅰ),emodin (Ⅱ),citreorosein (Ⅲ),chrysophanol 8-O-?-(6'-acetyl) glucopyranoside (Ⅳ),chrysophanol 8-O-?-D-glucopyranoside (Ⅴ),resveratrol (Ⅵ),9,9'-dianthranone-2,2'-dimethyl-5,5'-bis (?-D-glucopyranose)-9,9',10,10'-tetrahydro-4,4'-dihydroxy-10,10'-dioxo (trivial name:rumoside A) (Ⅶ),emodin-8-O-?-D-glucopyranoside (Ⅷ),resveratrol-3-O-?-D-glucoside (Ⅸ),and rutin (Ⅹ).Conclusion Compound Ⅶ is a new compound,named rumoside A.Compounds Ⅴ,Ⅷ,and Ⅹ are separated from R.gmelini for the first time.Compounds Ⅲ and Ⅳ are the compounds which have been found in the plants of Rumex L.for the first time.
ABSTRACT
Objective To study the effect of three fungi of Helminthosporium on the seven kinds of bioactive constituents in root of seedling Rumex gmelini. Methods Let the R. gmelini infect the fungi of Helminthosporium by dealing the leave surface of R. gmelini with fungal spore suspension. The content of seven kinds of bioactive constituents was determined by HPLC and the results were analyzed by variance analysis. Results During the treatment time, little effect of three fungi of Helminthosporium on content of polydatin, chrysophanol 1-glucoside, and physcion, but the obvious effect on resveratrol, musizin, emodin, and chrysophanol was found. Conclusion The effects of H. turcicum 001 on yield and content of resveratrol are significant in early-middle stage (0—40 d), but treatment time over 40 d is not suitable. The results of this study could provide the new idea and theoretical basis for the exploiting of natural medicines and the planting of Chinese herbal medicine.
ABSTRACT
Objective To compare the HPLC fingerprint of Rumex gmelini from different habitats by RP-HPLC (DAD). Methods In this paper, 12 different samples were studied. Separation was performed on an Planetsil C_ 18 column, with mobile phase consisting of methanol and 0.1% phosphoric acid-water and with gradient elution at the flow rate of 1.0 mL/min. The UV detection wavelength was 254 nm, column temperature was 35 ℃, and the analysis time was 50 min. Results The results showed that this method has a good repeatability and the ratio of common peaksarea of different samples had some difference. Conclusion This method can be used to establish the chromatographic fingerprint of R. gmelini with high specificity.