Traducción y validación de la Escala de Autoeficacia en Lactancia Materna BSES-SF en población colombiana / Translation and Validation of the Breastfeeding Self-Efficacy Scale bses-sfin a Colombian Population / Tradução e validação da Escala de Autoeficácia em Amamentação bses-sfna população colombiana

La autoeficacia en la lactancia materna es la confian-za de la madre en su capacidad para amamantar a su hijo(a), la cual se evalúa mediante el cuestionario Breastfeeding Self Efficacy Scale (bses). Diversos estu-dios han explorado las propiedades psicométricas del instrumento con resultados adecuados en diferentes países. Este estudio presenta el proceso de traducción y validación de la escala en su versión breve, de 14 ítems, en una muestra de mujeres colombianas. En total, se evaluaron 243 mujeres lactantes con hijos alrededor de los cuatro meses de edad. Se analizó la consistencia y estructura interna de la escala, así como su relación con otras variables. La escala evidenció adecuados indicios respecto a su consistencia interna (α = .91), se identificó una estructura factorial de dos dimensiones que explican un 48 % de la varianza, la relación con otras variables como la autoestima (ρ = .28) y el estrés percibido (ρ = .30) fueron débiles o moderadas, y las diferencias en las puntuaciones respecto a la historia obstétrica y las variables sociodemográficas fueron analizadas. Las evidencias psicométricas apoyan la utilización de la escala, tanto en el ámbito clínico como en el investigativo para evaluar la autoeficacia en mu-jeres lactantes colombianas

2Adriana Trujillo, Rubby Castro-Osorio, Natalia Maldonado-AvendañoAvances en Psicología Latinoamericana / Bogotá (Colombia) / Vol. 42(1) / pp. 1-20 / 2024 / ISSNe2145-4515obstétrica y las variables sociodemográficas fueron analizadas. Las evidencias psicométricas apoyan la utilización de la escala, tanto en el ámbito clínico como en el investigativo para evaluar la autoeficacia en mu-jeres lactantes colombianas.Palabras clave: autoeficacia; lactancia materna; bses-sf; psicometría.AbstractBreastfeeding self-efficacy is the mother's confidence in her ability to breastfeed her child and is assessed using the Breastfeeding Self-Efficacy Scale (bses). Several studies have explored the psychometric properties of the questionnaire with adequate results in different countries. The present study presents the translation and validation process of the 14-item brief version of the scale in a sample of Colombian women. In to-tal, 243 lactating women with children around four months of age were evaluated. The consistency and internal structure of the scale was analysed, as well as its relationship with other variables. The scale evidenced adequate internal consistency (α = .91), a factorial structure of two dimensions explaining 48 % of the variance was identified, the relationship with other variables such as self-esteem (ρ = .28) and perceived stress (ρ = .30) was weak or moderate, and the differences in scores regarding the obstetric history and sociodemographic variables were analyzed. The psychometric evidence supports the use of the scale in both clinical and research settings to assess self-efficacy in Colombian lactating women.

A autoeficácia na amamentação é a confiança da mãe em sua capacidade de amamentar seu filho e é avaliada por meio do questionário Breastfeeding Self Efficacy Scale(bses). Vários estudos exploraram as propriedades psi-cométricas do instrumento com resultados adequados em diferentes países. O presente estudo apresenta o processo de tradução e validação da escala em sua versão breve de 14 itens em uma amostra de mulheres colombianas. No total, foram avaliadas 243 lactantes com filhos em torno de quatro meses de idade. Foram analisadas a consistência e a estrutura interna da escala, bem como sua relação com outras variáveis. A escala apresentou indicações adequadas quanto à sua consistência interna (α = 0,91), foi identifi-cada uma estrutura fatorial bidimensional que explicou 48% da variância. A relação com outras variáveis como a autoestima (ρ = 0,28) e percepção ao estresse (ρ = 0,30) foi fraca ou moderada, e, foram analisadas diferenças nos escores referentes ao histórico obstétrico e às variáveis sociodemográficas. Evidências psicométricas apoiam o uso da escala tanto em ambientes clínicos como de pesquisa para avaliar a autoeficácia na amamentação em mulheres lactantes colombianas.

Impact du traitement par les antiviraux à action directe sur la qualité de vie des patients atteints d'hépatite C à Cotonou / Impact of treatment with direct-acting antivirals on quality of life in hepatitis C patients in Cotonou

Introduction: L'altération de la qualité de vie est l'une des conséquences de l'infection chronique au virus de l'hépatite C. L'objectif de cette étude était d'évaluer l'impact du traitement par les antiviraux à action directe (AAD) sur la qualité de vie des patients atteints d'hépatite C à Cotonou. Méthodes : Il s'agissait d'une étude transversale descriptive et analytique, avec un recueil de données à la fois rétrospectif et prospectif sur une période de 5 ans allant du 1er décembre 2015 au 1er septembre 2020. Etaient inclus tous les patients atteints d'hépatite C traités par les AAD dans la Clinique Universitaire d'hépato-gastroentérologie du Centre National Hospitalier et Universitaire Hubert Koutoukou Maga (CNHU-HKM) de Cotonou. L'efficacité était évaluée par la réponse virologique soutenue (RVS) à 12 semaines après la fin du traitement. Le questionnaire SF-36 était utilisé pour évaluer la qualité de vie des patients atteints d'hépatite C, avant et après traitement. Résultats : Pendant la période d'étude, 206 patients ont été colligés. Ils étaient constitués de 33,5% d'hommes et 66,5 % de femmes, soit une sex-ratio de 0,5. La moyenne d'âge des patients était de 62,0 ± 1,7 ans (extrêmes de 18 et 87 ans). La charge virale moyenne en UI/ml était de 3.507.336,9 ± 1.156.443,98 (extrêmes de 3422 et 75.674.348 UI/ml). Le score SF-36 était statistiquement plus élevé pour tous les items après le traitement par AAD (p˂0,05). Conclusion: Les antiviraux à action directe constitue un traitement qui améliore significativement la qualité de vie des patients atteints d'hépatite C à Cotonou.

Background: Impaired quality of life is one of the consequences of chronic infection with the hepatitis C virus. The aim of this study was to assess the impact of treatment with direct-acting antivirals (DAAs) on quality of life in patients with hepatitis C in Cotonou. Methods: This was a descriptive and analytical cross-sectional study, with both retrospective and prospective data collection over a 5-year period from 1 December 2015 to 1 September 2020. All patients with hepatitis C treated with DAAs at the University Hepato-Gastroenterology Clinic of the National and University Hospital Hubert Koutoukou Maga (CNHU-HKM) in Cotonou were included. Efficacy was assessed by the sustained virological response (SVR) at 12 weeks after the end of treatment. The SF-36 questionnaire, the reference measurement instrument, was used to assess the quality of life of hepatitis C patients before and after treatment. Results: A total of 206 patients were enrolled during the study period. They comprised 33.5% men and 66.5% women, giving a sex ratio of 0.5. The mean age of the patients was 62.0 ± 1.7 years, (extremes 18 and 87 years). The mean viral load in IU/ml was 3,507,336.9 ± 1,156,443.98 (extremes 3,422 and 75,674,348 IU/ml). The SF-36 score was statistically significantly higher for all items after DAA treatment (p˂0.05). Conclusion: Direct-acting antivirals are a treatment that significantly improves the quality of life of hepatitis C patients in Cotonou.

The effectiveness of home-based physical therapy on glycemic control and quality of life in adults with prediabetes

Background: Prediabetes is the preceding stage of diabetes which puts an individual to induce complications same as that of diabetes; hence, it should be treated to prevent its progression to diabetes and other consequences. However, there is very less literature about impact of home-based physical therapy on glycemic control and quality of life in Indian prediabetic population. Aims and Objectives: The aims of this study were to evaluate whether the home-based physical therapy shows effect on glycemic control and individual’s quality of life after a 3-month intervention. Materials and Methods: The study was conducted on 55 individuals who were diagnosed with prediabetes (36 males and 19 females) on basis of HbA1c level ranging from 5.7% to 6.4%. A 12-week exercise protocol was made which includes warm up exercises, main exercise program (aerobic and strengthening training), and cool down exercises along with dietary changes. SF-36 and HbA1c level is taken at baseline and at the end of 12 weeks for quality of life and glycemic control measures in participants, respectively. Results: The difference in HbA1c levels before and after treatment was found to be statistically significant. The HbA1c levels after the 3-month intervention showed significant reduction (P < 0.008) along with significant difference in all domain of SF-36 except in domain 3 (role limitation due to emotional problem) and domain 6 (social functioning). Conclusion: The home-based physical therapy program is effective in glycemic control and quality of life in adults with prediabetes.

Vitamin D is associated with a low quality of life: a primary care study

Introduction: Some studies have described impairment in quality of life of vitamin-deficient subjects. However, little is known about this association in primary care. This study aimed to evaluate the association between vitamin D deficiency and quality of life in postmenopausal women attending primary care in the municipality of Santa Maria ­ Brazil. Methods: A cross-sectional study was carried out with postmenopausal women over 55 years of age, accompanied in primary care, from March to August 2014. These women were randomly selected among the participants of a cohort study in the municipality of Santa Maria ­ Brazil. Data were collected through a standardized questionnaire, quality of life was assessed using the Short Form-36 Health Survey (SF-36), and 25-hydroxyvitamin D were measured using the ALPCO® ELISA method. Results: Of the total of 78 studied women, 11.54% had vitamin D deficiency. Women with vitamin D deficiency had a poorer quality of life assessed by SF-36. In the regression analysis, both vitamin D deficiency and falls were independently associated with a lower physical component of the SF-36. Conclusion: Vitamin D deficiency is associated with poorer quality of life in the studied postmenopausal women.

Preparation and Performance of Three-Dimensional Silk FibroinScaffolds with Different Matrix Stiffness / 医用生物力学

Objective After hydrogen bonding between collagen ( COL) and silk fibroin ( SF ) at different concentrations, a composite scaffold with adjustable stiffness was prepared by combining with gel system, and its physical and chemical properties were characterized. Methods SF with different qualities was dissolved in sodium alginate (SA) solution, then COL solution at different concentration and calcium carbonate ( CaCO3 ) powder were added. The hydrogels of SC1, SC2, and SC3 groups were obtained by taking out the mixed solution and adding some gluconic acid lactone ( GDL) powder, and different SF scaffolds were obtained after freeze drying. Results The SF scaffolds with adjustable stiffness were successfully prepared. The compression moduli of SC1, SC2, and SC3 groups were (17. 31±2. 73), (24. 12±1. 81), (32. 54±1. 81) kPa, respectively. The innerstructure of the scaffolds was observed. From SC1 group to SC3 group, pores of the scaffolds were smaller and fewer, and hydrophilicity of the materials become better and better. Conclusions Three-dimensional ( 3D) porous scaffolds with different matrix stiffness can be prepared by changing the concentration of SF and COL solution. The concentration of SF and COL is proportional to the compression modulus, water absorption, water retention and swelling rate of SF scaffolds, while inversely proportional to porosity. The findings of this study are expected to provide theoretical guidance for construction of scaffolds with appropriate matrix stiffness for inducing osteogenic differentiation of mesenchymal stem cells

Mapping the Constitution in Chinese Medicine Questionnaire to SF-6Dv1 / 中医杂志

ObjectiveTo construct and evaluate the transformation model of the Constitution in Chinese Medicine Questionnaire （CCMQ） to SF-6Dv1 health utility and broaden the applications of CCMQ. MethodsThe data of CCMQ and SF-6Dv1 were collected from 595 participants at baseline， 3 months and 6 months after the comprehensive intervention suitable for the corresponding traditional Chinese medicine （TCM） constitution. The estimation and validation datasets were constructed， and four statistical algorithms including the ordinary least squares （OLS）， MM robust regression （MM）， censored least absolute deviations （CLAD） and the Tobit model were used to create alternative models. The mean absolute error （MAE）， root mean square error （RMSE） and intraclass correlation coefficient （ICC） were used to evaluate the prediction performance of the model. ResultsThe constitution scores of all TCM constitutions by CCMQ was significantly correlated with the SF-6Dv1 health utility value measured at three timepoints； the health utility value of the SF-6Dv1 was positively correlated with gentleness type （r=0.596， r=0.578， r=0.606， all P＜0.05） and negatively correlated with eight unbalanced constitutions （r=-0.586~-0.301， all P＜0.05）. The MM established based on the subscale scores of CCMQ was the optimal mapping model， and the MAE， RMSE， and ICC values were 0.0741， 0.0930 and 0.766， respectively. Gentleness type， qi-deficiency type， phlegm-wetness type， qi-constraint type， and age were the primary factors included in the model. The measured and predicted value of SF-6Dv1 had a moderate positive correlation （r=0.673， r=0.617， P＜0.05） and a good consistency as shown by the Bland-Altman plot. ConclusionBy using MM， the CCMQ can be transformed into SF-6Dv1 health utility value for health economics analysis.

Growth and development status of children with severe β-thalassemia and its correlation with serum HGB and SF levels in Bazhong area / 公共卫生与预防医学

Objective To analyze the growth and development status of children with severe β-thalassemia major (β-TM) in Bazhong area and its correlation with hemoglobin concentration (HGB) and serum ferritin (SF) level, and to provide a theoretical basis for the diagnosis and treatment of children with β-TM in Bazhong area. Methods A total of 292 children with β-TM admitted to Bazhong Central Hospital from January 2019 to December 2020 were selected. The Z-score method was used to evaluate the growth and development of the children. According to the growth and development of the children, they were divided into the normal group (normal growth and development, n=163) and delayed group (growth and development delay, n=129). Another 60 healthy children were selected as the control group. The levels of HGB, serum SF, free triiodothyronine (FT3), free tetraiodothyroxine (FT4), and thyroid stimulating hormone (TSH) were compared among the three groups of children, and clinical data such as age, sex and age of onset were collected from the case system. Univariate analysis and logistic regression were used to analyze the independent risk factors of growth and development delay in β-TM children. Pearson correlation was used to analyze the correlation between growth retardation and HGB and serum SF levels in β-TM children. Results The serum SF and TSH levels in the delayed group were significantly higher than those in the normal group and the control group, while the levels of HGB and serum FT4 were significantly lower than those in the normal group and control group (P2000 ng/mL were risk factors for growth retardation in β-TM children (P2000 ng/mL, the height, weight, and HGB and serum SF levels should be monitored regularly.

Prognosis and correlation analysis of serum SAA, PCT and SF levels in children with severe mycoplasma pneumonia / 公共卫生与预防医学

Objective To analyze the prognosis of children with severe mycoplasma pneumonia (MPP) and its correlation with serum SAA, PCT and SF levels, so as to provide a basis for evaluating the prognosis of children with MPP. Methods A total of 273 children with MPP admitted to our hospital from January 2020 to December 2020 were divided into mild MPP children (n=187) and severe MPP children (n=86) according to the severity of their disease. According to the prognosis, children with severe MPP were divided into survival group (n=65) and death group (n=21). Serum SAA, PCT and SF levels were determined. Pearson correlation analysis was used to analyze the correlation between serum SAA, PCT and SF levels and APACHE ⅱ score. ROC curve was used to analyze the predictive value of serum SAA, PCT and SF levels for poor prognosis of children with severe MPP. Results The levels of serum SAA, PCT and SF and APACHE II score in children with severe MPP were significantly higher than those in children with mild MPP (P<0.05). Serum SAA, PCT and SF levels and APACHE II score in death group were significantly higher than those in survival group (P<0.05). Pearson correlation analysis showed that APACHE II score was positively correlated with serum SAA, PCT and SF levels (r =0.474,0.519,0.446,P<0.05). The AUC, sensitivity and specificity of combined ROC curve analysis to predict the prognosis of severe MPP were 0.871, 85.9% and 93.6% respectively, which were higher than those of SAA, PCT and SF alone. Conclusion SAA, PCT and SF are closely related to the prognosis of severe MPP, and can be used as potential markers to predict poor prognosis of severe MPP children.

Quality of life in patients after minimally invasive coronary artery bypass grafting surgery versus off-pump coronary artery bypass grafting surgery: A propensity score matching study / 中国胸心血管外科临床杂志

@#Objective    To compare and analyze the postoperative quality of life in patients after minimally invasive coronary artery bypass grafting (MICABG) and conventional median thoracotomy off-pump coronary artery bypass grafting surgery (OPCABG). Methods    From November 2015 to January 2018, 94 patients who underwent MICABG in the Peking University Third Hospital were included in the MICABG group. During the same period 441 patients who received OPCABG were included in the OPCABG group. The patients were matched by using propensity score matching method with a ratio of 1∶1. The quality of life was compared between two groups at 1 month, 6 months and 12 months after the surgery using SF-36 scale. Results    A total of 82 patients were matched for each group. In the MICABG group, there were 66 males and 16 females with a mean age of 62.6±8.2 years. In the OPCABG group, there were 67 males and 15 females with a mean age of 63.2±13.2 years. One month after the operation, the physical health assessment (PCS) and mental health assessment (MCS) of the MICABG group were higher than those of the OPCABG group (50.3±10.6 points vs. 46.1±10.3 points, P=0.011; 59.5±9.3 points vs. 54.2±11.0 points, P=0.002). Scores of these following five dimensions: general health, physical functioning (PF), role-physical, social functioning (SF), role-emotion in the MICABG group were higher than those in the OPCABG group, while the score of body pain was inferior to that in the OPCABG group, and the differences were statistically significant (P<0.05). Six months after the surgery, the PCS and MCS of the two groups were not statistically different (80.0±13.1 points vs. 77.8±12.4 points, P=0.271; 81.6±13.5 points vs. 80.4±11.2 points, P=0.537). However, the scores of PF and SF in the MICABG group were still higher than those in the OPCABG group (P<0.05). Twelve months after the surgery, there was no statistical difference in the score of each dimension between the two groups (P>0.05). Conclusion    The improvement of quality of life within 6 months after MICABG is better than that of OPCABG, and it is similar between the two groups at 12 months after the surgery, indicating that MICABG has a certain effect of improving the short-term quality of life after the surgery, and the long-term quality of life is comparable to conventional surgery.

Laboratory and clinical characteristics of patients with different SF3B1 genotypes in myelodysplastic syndromes / 中华检验医学杂志

Objective:To analyze the distribution of different SF3B1 genotypes in patients with myelodysplastic syndromes (MDS) and its prognostic value.Methods:Totally, 377MDS patients who were initially diagnosed in the First Affiliated Hospital of Nanjing Medical University from January 2014 to January 2022 were included in the retrospective analysis.The patients were divided into three different groups according to mutation stcote of SF3B1, including 317 patients with SF3B1 wild type (SF3B1 WT) (214 males and 103 females, 63(49, 71) years old),39 patients with SF3B1 K700E mutation(SF3B1 K700E(17 males and 22 females, 65(52, 73)years old)) and 21 patients with SF3B1 non-K700E mutation(SF3B1 non-K700E)(13 males and 8 females, 67(63, 73) years old). MDS-related 20 gene mutations were detected using targeted sequencing technology; Survival curves were constructed by the Kaplan-Meier method; Cox proportional hazards model was established to evaluate different factors at diagnosis on survival by univariate and multivariate analyses.. Results:Compared with SF3B1 non-K700E patients, SF3B1 K700E patients had a higher median absolute neutrophil count ( P=0.002) and were likely to be in the low/int-1 International Prognostic Scoring System (IPSS) categories ( P=0.023). A 20-gene targeted sequencing analysis showed that, compared with SF3B1 WT patients, SF3B1 K700E patients were associated with lower frequency of ASXL1 and U2AF1 mutations ( P=0.018 and P=0.003); while compared with SF3B1 non-K700E patients, the frequency of ASXL1 mutation was significantly lower in SF3B1 K700E cases ( P=0.029). Patients with SF3B1 K700E had better overall survival (OS) in comparison with SF3B1 WT and SF3B1 non-K700E in MDS patients ( P<0.001 and P=0.045, respectively). In comparison with SF3B1 WT patients, SF3B1 MUT patients had more favorable OS and progression-free survival (PFS) in MDS without excess blasts ( P<0.001 and P<0.001, respectively), but no significant difference was found in MDS with excess blasts ( P>0.05). Compared with SF3B1 WT patients, SF3B1 K700E patients had superior OS and PFS in the int-1 IPSS category ( P=0.010 and P=0.013, respectively). By multivariable analysis, the presence of SF3B1 K700Ewas an independent predictor of superior OS ( HR=0.461,95% CI 0.262-0.811, P=0.007). Conclusion:SF3B1 K700E and SF3B1 non-K700E patients had significantly improved OS in comparison with SF3B1 WT MDS patients. Furthermore, SF3B1 K700E patients were associated with a better OS compared with SF3B1 non-K700E MDS patients. SF3B1 mutation could not overcome the poor prognostic effect of excess blasts, which highlights the importance of the SF3B1 mutation subtype in risk assessment of MDS without excess blasts.

Comparison of the application of WOMAC, SF-36 scales and the "Assessment for Therapeutic Efficacy on Kashin-Beck Disease" (WS/T 79-2011) standard in the evaluation of efficacy in patients with Kashin-Beck disease / 中华地方病学杂志

Objective:To compare the application effect among Western Ontario and McMaster University Osteoarthritis Index (WOMAC) scale, Medical Outcomes Study 36-item Short Form (SF-36) scale and "Assessment for Therapeutic Efficacy on Kashin-Beck Disease" (WS/T 79-2011) standard in the evaluation of therapeutic effect of patients with Kashin-Beck disease, which could provide basis for the treatment evaluation of patients with Kashin-Beck disease.Methods:A total of 213 patients with Kashin-Beck disease in Gansu Province were investigated. WOMAC scale, SF-36 scale and standard of WS/T 79-2011 were used to analyze the quality of life of patients before and after treatment. The reliability, construct validity, content validity, discriminant validity of WOMAC and SF-36 scales were compared. Correlation between WOMAC, SF-36 scales and standard of WS/T 79-2011 were evaluated.Results:Both WOMAC and SF-36 scales had good construct validity and content validity (construct validity showed WOMAC and SF-36 scales contained 1 and 2 common factors, respectively; content validity showed WOMAC and SF-36 scales contained 3 and 8 common factors, respectively). The reliability and discriminant validity of WOMAC scale were better than those of SF-36 seale (reliability showed WOMAC reliability coefficient ≥0.934, the reliability coefficient of SF-36 scale was ranged from 0.386 to 0.999. Discriminant validity showed there were differences in 3 dimensions of the WOMAC scale before and after treatment, while there were differences in 6 out of 8 dimensions of the SF-36 scale). The correlation coefficients between WOMAC scale and standard of WS/T 79-2011 ranged from 0.175 to 0.437, the correlation coefficients between SF-36 scale and standard of WS/T 79-2011 ranged from - 0.434 to - 0.099 ( P < 0.05). Conclusion:The reliability, discriminant validity and correlation with the standard of WS/T 79-2011 of WOMAC scale are better than those of SF-36 scale in efficacy evaluation of patients with Kashin-Beck disease.

A Preliminary Content Validity Study of the Malay Version of READI-SF (Kajian Awal Kesahan Kandungan Versi Bahasa Melayu bagi READI-SF) / Jurnal Sains Kesihatan Malaysia

@#Readiness for treatment reflects the individual’s motivation to seek help and preparedness to engage in treatment activities. The READI-SF aimed to assess parental readiness to engage in treatment. The present study aimed to translate the English version of READI-SF into the Malay language and subsequently assess its content validity using the content validity ratio (CVR), content validity index (CVI) and modified kappa analyses. Four independent translators carried out the translation procedures. Eight clinical professionals were involved as content experts. Of 17 items, six items needed to be revised due to low CVR values (< 0.75). Based on unfavourable individual CVI (I-CVI) results (< 0.78), two items (for item relevancy) and six items (for item clarity) had to be modified. The overall CVI of the questionnaire (S-CVI) was found to be good (i.e., 0.91 for item relevancy and 0.83 for item clarity). Nine items needed to be revised, and the modifications were carried out accordingly. At the end of the study, a valid Malay-translated READI-SF was produced. It has good potential to serve as a clinical tool to assess the readiness of Malay-speaking parents to engage in treatment. However, prior to its intended clinical application, further research is warranted to assess other aspects of validity (e.g., face validity, construct validity, and criterion-related validity), as well as READI-SF reliability.

Secretory expression of human thyroid stimulating hormone receptor A subunit in sf9 insect cell system / 西安交通大学学报(医学版)

【Objective】 To construct the secretory expression system of insect cells to express the secretory TSHR A subunit protein in the ovarian cells of Spotoma oryzae (sf9). 【Methods】 A recombinant plasmid containing the target protein was constructed, and then the positive bacmid was screened out by the blue and white spots experiment. The verified bacmid was transfected into SF9 insect cells to obtain recombinant baculovirus. The virus was amplified, and the titer level was detected by virus plaque assay. Finally, Western blotting was used to identify the expression of the recombinant protein and optimize the expression conditions. 【Results】 During the construction of the protein expression system, PCR identification and sequencing results confirmed the correctness of the sequences of the recombinant plasmid and the recombinant bacmid. After the transfection of the bacmid, the signs of virus budding were observed in sf9 cells. The virus was collected and amplified. The titer of P1 generation virus was 2×107 pfu/m according to the plaque assay. The recombinant protein was identified by Western blotting and confirmed to be exogenous into the culture medium. The optimal condition for virus infection and protein expression was 72 h after the infection when the multiplicity of infection (MOI) was 1. 【Conclusion】 We constructed an insect cell expression system secreting TSHR 22-289 (55 ku), and the protein could be successfully glycolyzed. This system provides a preliminary basis for the construction and production of its industrial platform and also provides a useful tool for studies on TSHR protein and prevention of GO in the future.

Mejoría del dolor, la función y la calidad de vida en dolor lumbar subagudo: ensayo clínico controlado con ejercicio vs. AINES / Improvement of pain, function and quality of life in subacute low back pain: controlled clinical trial with exercise vs. NSAIDs

Introducción. El dolor lumbar es una de las causas más frecuentes de consulta y discapacidad en pacientes, y según su evolución temporal se puede clasificar como agudo, subagudo y crónico. Objetivo. Estimar en pacientes con Dolor Lumbar Subagudo (DLS), la eficacia de un programa de ejercicio comparado con antiinflamatorios no esteroideos (AINES). Métodos. Se realizó un ensayo clínico controlado aleatorio, con enmascaramiento simple en 90 pacientes y DLS con o sin radiculopatía, 46 pacientes fueron asignados a un programa de ejercicio físico y 44 a tratamiento con AINES. El desenlace primario fue la mejoría del dolor y los secundarios mejoría en la función, calidad de vida, ausentismo laboral y depresión con seguimiento a 1, 3 y 6 meses. Resultados. Al mes, no se registró diferencias en el dolor entre los grupos de 8,16 (IC 95 % -2,19 a 18,51), sin embargo, en el grupo de ejercicios hubo una mejoría de 47,3 (SD: 19,8) a 28,8 (SD: 20,5), p <0,001, y en el grupo de AINES de 45,2 (SD: 22,6) a 34,9 (SD: 25,0), p = 0,018. Otras muestras de mejoría se observaron en la función medida por el Índice de Discapacidad de Oswestry (ODI), la cual mejoró al mes en el grupo de ejercicio (p<0,001), mientras,la función física también mejoró al mes en el grupo de ejercicio (p= 0,038). Otra mejoría se observó en el dolor, función y calidad de vida que se mantuvo a los 3 y 6 meses en ambos grupos. Finalmente, La recurrencia fue mayor en el grupo de AINES: 25,5 % vs. 7,1 % (p= 0,04) al mes; 25,5 % vs. 7,1 % (p= 0,04) y 20,5 % vs. 5 % (p= 0,04), a los 3 y 6 meses. Conclusión. El ejercicio supervisado fue más efectivo que los AINES para disminuir la discapacidad y las recurrencias y mejorar la función física en pacientes con DLS.

Introduction. Low back pain is one of the most frequent causes of consultation and disability in patients, and according to its temporal evolution it can be classified as acute, subacute and chronic. Objective. To estimate the efficacy of an exercise program compared to non-steroidal anti-inflammatory drugs (NSAIDs) in patients with subacute low back pain (LBP). Methods. A randomized, single-masked, controlled clinical trial was conducted in 90 patients and DLS with or without radiculopathy, 46 patients were assigned to a physical exercise program and 44 to NSAID treatment. The primary outcome was improvement in pain and the secondary outcomes were improvement in function, quality of life, work absenteeism and depression with follow-up at 1, 3 and 6 months. Results. At 1 month, there was no difference in pain between groups of 8.16 (95 % CI -2.19 to 18.51), however, in the exercise group there was an improvement from 47.3 (SD: 19.8) to 28.8 (SD: 20.5), p <0.001, and in the NSAID group from 45.2 (SD: 22.6) to 34.9 (SD: 25.0), p = 0.018. Other signs of improvement were seen in function as measured by the Oswestry Disability Index (ODI), which improved at 1 month in the exercise group (p<0.001), while physical function also improved at 1 month in the exercise group (p= 0.038). Another improvement was observed in pain, function and quality of life which was maintained at 3 and 6 months in both groups. Finally, recurrence was higher in the NSAID group: 25.5 % vs. 7.1 % (p= 0.04) at 1 month; 25.5 % (p= 0.04) at 1 month; 25.5 % (p= 0.038) in the exercise group (p= 0.038) at 1 month.

Catquest-9SF questionnaire shows greater visual functioning in bilateral cataract populations: A prospective study

Purpose: Visual functioning evaluated by the Catquest?9SF questionnaire has shown to be a valid measure for assessing a patient抯 prioritization for cataract surgery. This study adapted Catquest?9SF for visual function outcomes post uni?lateral cataract surgery or bi?lateral cataract surgery. Methods: Visual functioning was assessed before and after uni?lateral or bi?lateral cataract surgery using the Catquest?9SF questionnaire. Patients were enrolled to this study prior to their cataract surgery between March 29 and April 30, 2021 at Shellharbour Hospital, Australia. Catquest?9SF questionnaires were completed prior to and 3 months post surgery. Resulting data were assessed for fit to a Rasch model using WINSTEPS software (version 4.2.0). Catquest?9SF data analysis of Chi?square, Wilcoxon sum test, and Fischer抯 test were performed in R (version 4.1.0). P value <.05 was considered statistically significant. Results: Sixty?one patients (mean age = 73.2 years, 62% female) were included for analysis. Catquest?9SF response thresholds, adequate precision (person separation index = 2.58, person reliability = 0.87, Cronbach抯 alpha = 0.74), uni?dimensionality, and no misfits (infit range 0.65�33; outfit range 0.64�31) were recorded. The mean of item calibration for patients was ?0.22 post?operatively. There was significant (P <.05) improvement (16.3%) in visual functions across all nine Catquest 9?SF items. There was a significant mean visual function difference between patients with uni?lateral (10.1%) and bi?lateral cataract surgery (22.3%) pre?operatively and post?operatively. Conclusion: The Catquest?9SF questionnaire showed excellent psychometric properties and can assess visual functioning in an Australian population. There was a significant improvement in patient visual function post cataract surgery and higher functioning with bi?lateral cataract surgery

Impacto de un programa de ejercicio físico domiciliario de intensidad leve-moderada sobre calidad de vida, fuerza, resistencia aeróbica, equilibrio y flexibilidad en pacientes adultos mexicanos trasplantados de riñón

Contexto en México, la enfermedad renal crónica presenta un aumento exponencial y el trasplante renal se ha colocado como la mejor opción de tratamiento. A pesar de ello, los receptores de trasplante presentan una menor capacidad funcional y calidad de vida comparándolos con individuos sanos, lo cual se asocia a un estilo de vida sedentario, sumado con los efectos musculoesqueléticos de los inmunosupresores, sin embargo, la investigación es limitada. Objetivo evaluar el impacto en calidad de vida, fuerza, resistencia aeróbica, equilibrio y flexibilidad de un programa domiciliario de ejercicio físico de intensidad leve-moderada, en pacientes adultos mexicanos trasplantados de riñón. Metodología participaron nueve pacientes (29 ± 5,2 años), a los cuales se les evaluó la fuerza de agarre manual mediante un dinamómetro; la flexibilidad mediante la prueba de "distancia dedos planta" (sit-and-reach test por su nombre en inglés); su agilidad-equilibrio y fuerza en miembro inferior mediante la prueba de "pararse-sentarse", y "levántate y anda" (sit-to-stand y get-up-and-go por sus nombres en inglés) respectivamente; su actitud cardiorrespiratoria mediante el test de marcha por seis minutos, y su calidad de vida mediante el cuestionario de salud SF36. Resultados se mostraron mejoras estadísticamente significativas en dinamometría manual (P 0,005), "distancia dedos planta" (P 0,016), sentarse y pararse (P 0,0011) y test de marcha por seis minutos (P 0,012). En el cuestionario SF36, hubo mejoría en la función física (P 0,03) y salud general (P 0,01), pero no hubo cambios en los análisis de laboratorio y la tasa de filtración glomerular. Conclusiones un programa domiciliario de ejercicio combinado de intensidad leve-moderada parece tener un efecto positivo sobre la capacidad funcional y la calidad de vida de los receptores de trasplante renal, sin embargo, es necesaria una mayor investigación para evaluar la adherencia al programa.

Background in Mexico, chronic kidney disease presents an exponential increase, kidney transplantation has become the best treatment option. Despite this, transplant recipients have lower functional capacity and quality of life compared to healthy individuals, which is associated with a sedentary lifestyle, added to the musculoskeletal effects of immunosuppressants. However, the research is limited. Purpose to evaluate the impact of a home program of strength exercises, aerobic endurance, balance and flexibility of mild-moderate intensity in transplant patients. Methodology 9 patients (29.5, 2 years) participated, manual grip strength was evaluated by means of a dynamometer, flexibility through the sit and reach test, agility-balance and strength in the lower limb by means of the "sit to stand" and the "get up and go" respectively, functional capacity through the 6-minute walk test, and quality of life through the SF36 health questionnaire. Results Statistically significant improvements were shown in manual dynamometry (P 0.005), sit and reach test (P 0.016), Sit to stand test (P0.0011), 6-minute walk test (P 0.012). In the SF36 questionnaire, there was improvement in physical function (P0.03) and general health (P0.01), there were no changes in laboratory tests and glomerular filtration rate. Conclusion a home program of combined mild-moderate intensity exercise seems to have a positive effect on the functional capacity and quality of life of kidney transplant recipients, however, more research is needed to evaluate adherence to the program.

Trastornos mentales y calidad de vida relacionada con la salud en profesionales de Unidades de Cuidados Intensivos / Mental disorders and health-related quality of life in Intensive Care Units professionals

Resumen Los profesionales de la salud de las Unidades de Cuidados Intensivos (UCI) enfrentan situaciones de sufrimiento humano, competitividad y demanda, que podrían perjudicar su calidad de vida y su salud mental. El objetivo del estudio fue describir los trastornos mentales comunes y la calidad de vida relacionada con la salud en profesionales de la salud de la UCI en Salvador, Brasil. Este estudio transversal con 195 profesionales utilizó un cuestionario sociodemográfico y laboral, el Self-Reporting Questionnaire (SRQ-20) de trastornos mentales comunes y el 36-Item Short Form Health Survey (SF-36v2) de calidad de vida relacionada con la salud. El 29.7 % de los profesionales presentaron trastornos mentales comunes, especialmente entre profesionales de enfermería (RP = 2.28; IC 1.19-4.39; p = .007). La calidad de vida relacionada con la salud para todos los profesionales estuvo disminuida, principalmente en función social (44.25 ± 10.15) y rol emocional (45.86 ± 10.58). El SRQ-20 correlacionó fuertemente con los dominios dolor corporal (r = -.502), salud general (r = -.526), vitalidad (r = -.656), función social (r = -.608), salud mental (r = -.631) y el componente de salud mental (r = -.638) del SF-36v2 (p < .01). Los profesionales con trastornos mentales comunes mostraron una calidad de vida relacionada con la salud más deteriorada, esencialmente en dominios del componente de salud mental, y refirieron también dolor corporal. Es necesario discutir e implementar estrategias de evaluación, prevención y promoción de la salud mental entre los profesionales de las UCI para que sean consideradas dentro de las políticas de salud laboral.

Comprometimento da qualidade de vida em pacientes com acalásia chagásica e fatores clínicos associados / Impaired quality of life in chagas acalasia patients and associated clinical factors

ABSTRACT Background: No study has focused on Health-Related Quality of Life (HRQoL) for Chagas Achalasia patients. Objective: To compare HRQoL between Chagas Achalasia patients and the general population; and to correlate HRQoL with clinical factors that can affect it. Methods: Sixty Chagas Achalasia patients and 50 controls were evaluated. All patients underwent esophageal manometry for the diagnosis of achalasia and esophagogram to determine the grade of megaesophagus. Three questionnaires were used: 1) clinical: the following data were collected: demographic, medical history, body mass index, occurrence of six esophageal symptoms (Esophageal Symptom Score: number of symptoms reported by patients), duration of dysphagia; 2) socio-economic-cultural status evaluation: patients and controls answered seven questions about their socio-economic-cultural conditions; 3) HRQoL: the validated Brazilian-Portuguese version of the Short-form Health Survey (SF-36) questionnaire (license QM020039) was used. It measures health in eight domains: 3a) four physical: physical functioning, role limitations relating to physical health, bodily pain, and general health perception; 3b) four mental: vitality, social functioning, role limitations relating to emotional health, and mental health. These domains can be summarized into Physical and Mental Summary scores. We analyzed correlations between SF-36 Physical/Mental Summary Component scores and the following clinical factors: Esophageal Symptom Score, duration of dysphagia, body mass index, grades of megaesophagus (defined by the esophagogram) and presence/absence of megacolon (defined by opaque enema). Results: Patients and controls had similar age, gender, medical history, and socio-economic-cultural lifestyles (P>0.05). All patients had dysphagia and megaesophagus. SF-36 scores were significantly lower in Chagas Achalasia patients than controls for all eight domains (physicals: P<0.002; mentals: P<0.0027). The Physical and Mental Summary Component scores were also lower in Chagas Achalasia patients than controls (P<0.0062). For patients, the Physical Summary score was negatively correlated to Esophageal Symptom Score (P=0.0011) and positively correlated to body mass index (P=0.02). No other correlations were found. Conclusion: Chagas Achalasia patients have an impaired HRQoL in all physical and mental domains. Patients reporting more symptoms had worse physical domains. Patients with higher body mass index had better physical domains.

RESUMO Contexto: Não encontramos na literatura estudos sobre a qualidade de vida em pacientes com acalásia chagásica especificamente. Objetivo: Comparar a qualidade de vida de pacientes com acalásia chagásica e a da população em geral. Também, correlacionar a qualidade de vida nestes pacientes com fatores clínicos que possam afetá-la. Métodos: Estudamos 60 pacientes com acalásia chagásica e 50 controles. Todos os pacientes foram submetidos à manometria esofágica para diagnóstico de acalásia e esofagograma técnica padrão para determinar o grau do megaesôfago. Usamos 3 questionários: 1) clínico: foram coletados os seguintes dados: demográficos, história clínica, índice de massa corporal, presença de seis sintomas esofágicos (definimos Escore de Sintomas Esofágicos como o número de sintomas relatados pelos pacientes), duração da disfagia; 2) avaliação sócio-econômico-cultural: sete questões sobre as condições sócio-econômico-culturais foram perguntadas para pacientes e controles; 3) qualidade de vida: foi avaliada pelo questionário SF-36, versão validada para o português-Brasil (licença QM020039). Este é um questionário genérico que mede a qualidade de vida em oito domínios: 3a) quatro físicos: capacidade funcional, aspectos físicos, dor corporal, estado geral de saúde; 3b) quatro mentais: vitalidade, aspectos sociais, aspectos emocionais, saúde mental. Estes oito domínios podem ser compilados em dois escores: Sumário dos Escores Físicos e Sumário dos Escores Mentais. Na análise de fatores clínicos que pudessem afetar a qualidade de vida dos pacientes, avaliamos: escores de sintomas esofágicos, duração da disfagia, índice de massa corporal, graus de megaesôfago e presença/ausência de megacólon. Resultados: Os dois grupos (pacientes e controles) apresentaram semelhantes idade, gênero, história médica e condições socioeconômico-culturais (P>0,05). Todos os pacientes tinham disfagia e megaesôfago. Com relação à qualidade de vida, pacientes com acalásia chagásica apresentaram valores significativamente menores do que os controles em todos os domínios do questionário SF-36 (domínios físicos: P<0,002; domínios mentais: P<0,0027). Os Sumários dos Escores Físicos e Mentais também foram significativamente menores em pacientes do que nos controles (P<0.0062). A análise dos fatores clínicos que poderiam afetar a qualidade de vida nos pacientes mostrou que o Sumário dos Escores Físicos se correlaciona negativamente com o Escores Dos Sintomas Esofágicos (P=0,0011) e positivamente com o índice de massa corporal (P=0,02). Não observamos qualquer outra correlação. Conclusão: Pacientes com Acalásia Chagásica têm pior qualidade de vida que a população em geral, em todos os domínios físicos e mentais. Pacientes que relataram mais sintomas apresentaram pior qualidade de vida nos domínios físicos. Pacientes com valores maiores de índice de massa corporal apresentaram melhor qualidade de vida nos domínios físicos.

Ayurvedic Management of Moderate Depressive Disorder- A Case Report

Depressive disorders are highly prevalent mental disorders characterized by sadness, loss of interest or pleasure, feelings of guilt or low self-esteem, disturbed sleep or appetite, feelings of tiredness and poor concentration. About 300 million people are estimated to suffer from depression at a global level and in India the figures approximate to about 45.7 million. As per WHO, depression will be the leading cause of burden of disease by 2030 and the prevalence, incidence, and the morbidity risk of depression appears to be more in females than males. A 42-year-old lady presented with primary concerns of increased thoughts, decreased sleep, discomfort in stomach, abdominal distension, increased fatigue, decreased appetite and increased tension. Detailed interview with her husband and parents revealed that she had increased thoughts, slowness in performing activities, inability to do day to day works, reduced memory and concentration, increased anger towards her husband, mother and daughter, reluctance to have food and reduced social mingling. Based on the observations made in the level of affective and behavioural domains, diagnosis of depressive disorder - current episode moderate was eventually done as per the diagnostic criteria mentioned in International Classification of Disease 10. In Ayurveda, the condition was diagnosed as Kaphaja Unmada based on the typical symptoms. The treatment protocol including Snehapana, Vamana, Virechana and Vasthi was administered. The mental status of the patient was also addressed during the treatment period and Satvavajaya measures including individual and family counseling methods were administered. There was significant improvement in the Becks Depression Inventory score and Q – LES – Q – SF questionnaire after the intervention.

Quality of Life of Elderly People Living in Different Types of Long-term Care Facilities

Abstract Population aging is a worldwide occurrence that has become urgent in developing countries. Quality of life can be measured to identify functional capacity and determine the degree of impact on quality of life exerted on an individual. This study aimed to measure the quality of life of elderly people who have been living in different long-term care facilities for the elderly. Therefore, this research was developed with a qualitative and descriptive approach. Data were obtained from medical records and interviews, and were analyzed in R language interpreter software on the Ubuntu Linux operating system. Seventy-nine elderly people participated in the study. Eleven lived in Home Marista, and 68 lived in Home Jesus Maria José. A socioeconomic questionnaire was used to collect data on the socioeconomic characteristics of the participants. The Mini-Mental State Exam was used to measure degree of cognition. The Short Form-36 Questionnaire was used to measure quality of life. The present study suggests that the results found can clarify the individual vulnerability of the elderly in the age group studied regardless of the type of residence. Despite the discrepancy found regarding the care provided at the long-term care facilities, the scores, obtained with the quality of life assessment, did not show significant differences.