ABSTRACT
Objective: To detect the expression of sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (SPOCK 1) gene in bladder cancer cells, and further to investigate the effects of down-regulation of SPOCK 1 gene expression on the proliferation, migration and invasion of bladder cancer cells. Methods: The expression level of SPOCK 1 gene in human ureteral epithelial immortalized cell line SV-HUC-1 and bladder cancer cell lines 5637 and T24 was detected by real-time fluorescent quantitative PCR (RFQ-PCR) and Western blotting, respectively. The siRNA fragments targeting SPCOK 1 gene were constructed and transfected into bladder cancer 5637 cells by liposome (as the experimental group); at the same time, the 5637 cells were transfected without anything as the blank control group and transfected with scramble siRNA as the negative control group. Then the expressions of SPOCK1 mRNA and protein in each group were detected by RFQ-PCR and Western blotting, respectively. The changes of proliferation, migration and invasion abilities of bladder cancer 5637 cells after SPOCK1 expression down-regulation were detected by CCK-8 method, Wound healing assay and Transwell chamber assay, respectively. Results: As compared with the ureteral epithelial immortalized cells, the expression levels of SPOCK1 mRNA and protein were higher in bladder cancer cells, especially in 5637 cells (both P < 0.05). After transfection with SPOCK1 siRNA, the expression of SPOCK 1 gene was downregulated (P < 0.05), the proliferation, migration and invasion of bladder cancer 5637 cells were significantly inhibited (all P < 0.05). Conclusion: SPOCK 1 gene is overexpressed in bladder cancer cells. The down-regulation of SPOCK 1 gene expression can reduce the proliferation, migration and invasion of bladder cancer 5637 cells.
ABSTRACT
Testicular proteoglycan 1 ( SPOCK1 ) , a kind of extracellular matrix glycoprotein, can inhibit the activity of cathepsin and promotes the combination of low affinity for calcium.SPOCK1 is essential to the de-velopment of mammals.It not only regulates cell,and the interactions between cells and matrix are also related to cell migration and proliferation.Currently,SPOCK1is confirmed that expression can promote the occurrence and development of tumor,suggesting it could be a new anti-tumor target.This article is to review SPOCK1 molecular structure,biological function and its relationship with tumor.