ABSTRACT
To investigate the effects of Baoganning on injury of liver cells induced by hydrogen peroxide (H 2O 2) in rats.Rat models of injury of liver cells were induced by H 2O 2 at final concentration of 2 mmol/L. Six kinds of suspension of isolated rat liver cells were prepared according to the addition of serum. They were group A ( blank control), group B (H 2O 2 model), group C (normal control), group D (vitamin E), group E (low dosage of Baoganning) and group F (high dosage of Baoganning). Superoxide dismutase (SOD) activity, nitric oxide (NO) and malonyl dialdehyde (MDA) contents and glutamic pyruvic transaminase (ALT) level were detected.SOD activity and NO and MDA contents were increased and ALT level was decreased in Baoganning groups.[Conclusion]Baoganning exerts a protective effect on injury of liver cells induced by H 2O 2 in rats.
ABSTRACT
To study the protective effect of Shengmai Injection on adriamycin-induced cytotoxity of diaphragm in rabbits. The rabbits were randomly allocated to three groups. The control group were treated with NS 2 ml?kg -1 for five days, adriamycin+NS group with NS 2ml?kg -1 for five days and then administered adriamycin 10 mg?kg -l and adriamycin + Shengmai Injection group with Shengmi Injection 2 ml?kg -l for five days and then administered adriamycin 10 mg?kg -1 . Twenty-four hours later, animals in the three groups were anaesthetized. Then the transdiaphragmatic pressure (Pdi) and diaphragm evoked potential(DEP) were measured, SOD activity and MDA content were tested and the ultrastructure of diaphragm cells was observed under electron microscope. Adriamycin could lower the Pdi and amplitude of DEP (P
ABSTRACT
[Objective] To evaluate the therapeutic effect of Buxin Huoluo Capsule (BHC) for coronary heart disease (CHD) and to explore its anti-lipid-peroxidation mechanism. [Methods] One hundred and seventy-five cases of CHD were treated with BHC and 121 cases with isosorbide dinitrate (ID) . Effects of BHC on angina pectoris, electrocardiograph, superoxide dismutase (SOD) activity and lipid peroxides (LPO) level were observed. [Results] In BHC group, the total effective rate in relieving angina pectoris was 88.0 % and that in improving electrocardiogram was 80.0 % , the difference being not significant as compared with ID group. As for the reduction of frequency of angina pectoris and the decrease of dosage of nitroglycerin, BHC were superior to ID. Furthermore, BHC decreased LPO level and increased SOD activity, the difference being significant (P
ABSTRACT
This study observed the relationship between active oxygen radicals and early lung injury after smoke inhalation injury in awake goats (N = I5) with chronic lung lymph fistular. We measured superoxide dismutase (SOD) to demonstrate the active oxygen radicals; Lung lymph flow (QL), lung lymph/plasma total protein and albumin ratio (L/P) , lung lymph total protein clearance (CL) , lung transvascu-lar protein flux, extravascuiar lung water (EVLW) , as well as PaO2 as parameters indicating lung injury.The result demonstrated that during the earlier postinjury period the injuried animals were suffering from hypoxemia, matabolic acidosis and respiratory alkalosis. EVLW and QL increased sharply during the first hour. Interstitial and alveolar edema were seen under microscope. 'These results indicated that the animals develop lung edema and acute respiratory failure. Changs of QL and EVLW were similar to L/P, CL and lung transvascular protein flux; the correlation between L/P and EVLW was significant. These data showed that the pulmonary edema was mainly a permeability one. Changes of active oxygen radicals of lung lymph fluid and arterial blood were earlier than that of venous blood; and active oxygen radicals of arterial blood was slightly higher than that of venous blood. The active oxygen radicals in pulmonary tissue and fluid of lung lavage and the lung lymph SOD clearance increased immidiatly in postinjury. These data suggested that active oxygen radicals were increased and produced in lungs. The active oxygen radicals changed with EVLW, QL, L/P and lung transvascular protein fluxi the correlation between EVLW and active oxygen radicals in pulmonary tissue, fluid of lung lavage, lung lymph SOD clearance and lung transvascular protein flux were all significant. These observations showed that early after smoke inhalation injury active oxygen radicals played a possible role in the development and progression of lung injury and pulmonary edema.