ABSTRACT
Objective To evaluate the association of the SYK gene promoter-803A T polymorphism and breast cancer sus ceptibility during Han descent female population in Beijing area.Methods In this case control study,genotype of SYK (A＞T at-803,rs290987) was determined by polymerase chain reaction combined with DNA direct sequencing in blood samples of 57 breast cancer cases and 60 age matched bealthy controls.They were all female.Genotype and allele frequency distribu tions of 803A T were determined and analyzed.ELISA test was performed to detect the serum level of SYK in the two groups.Results The frequencies of the T allele and TA+TT genotypes of the 803A＞T were found to be significantly higher in the breast cancer patients in contrast to the healthy individuals of the control group(x2 =5.348,P=0.021).Pa tients with the T allele (TA+TT) had a 2.87-fold risk of developing breast cancer compared with those with the A allele (AA) (OR=2.87;95％ CI=1.27～6.49).The test group had significantly decreased level of SYK in serum compared to normal controls (F 33.278,P＜0.01).Conclusion This study revealed that the genotype TA and TT of the SYK gene-803 loci of Han female population in the Beijing area are the risk factors for the breast cancer.
ABSTRACT
Objective To detect methylation of spleen tyrosine kinase (SYK)gene promoter region in nasopharyngeal carcino-ma,and to explore relationship between carcinogenesis of nasopharyngeal carcinoma and methylation of SYK gene promoter region.Methods A total of 52 patients with nasopharyngeal carcinoma and 26 patients with chronic rhinitis from Baoan People’s Hospital of Shenzhen Hospital of Peking University were enrolled in this study between February 2012 and August 2014.All cases were diagnosed by pathological examination.Methylation specific-polymerase chain reaction assay was per-formed to detect methylation status of SYK gene promoter region,the rate of methylation was compared for patients with nasopharyngeal carcinoma and patients with chronic rhinitis.Results The methylation of promoter region of SYK gene was detected for 11 biopsy samples among 52 nasopharyngeal carcinoma patients,the methylation frequency was 21.2% in naso-pharyngeal carcinoma biopsy samples,while methylation was not found in 26 chronic rhinitis biopsy samples.Conclusion Low methylation of promoter region of SYK gene was found in nasopharyngeal carcinoma.It suggests that methylation of SYK gene promoter region may contribute to carcinogenesis of nasopharyngeal carcinoma.