ABSTRACT
The present study evaluated the efficacy of the mineralizing action of Casearia sylvestris ethanolic extract on bovine dentin blocks in its pure form and in dental paste, through scanning electron microscopy. The dentin blocks were immersed in artificial saliva and incubat ed at 37°C for 7 days. Subsequently, six groups were treated with different test substances and analysed qualitatively and quantitatively at 30 and 60 days. The tests used were Kruskal - Wallis and Dunn's. Shapiro - Wilk and ANOVA. The qualitative analysis at 30 days showed a difference between the groups treated with ethanolic extract and toothpaste. Quantitatively, at 30 days, treatment with ethanolic extract of Casearia showed a greater number of open dentinal tubules. At 60 days, the difference persisted on ly for the blocks treated with toothpaste. The results obtained indicated that there is a positive relationship between the use of Casearia sylvestris and obliteration of dentinal tubules
El presente estudio evaluó la eficacia de la acción mineralizante del extracto etanólico de Casearia sylvestris sobre bloques de dentina bovina en su forma pura y en pasta dental, mediante microscopía electrónica de barrido. Los bloques de dentina se sumergieron en saliva artificial y se incubaron a 37°C durante 7 días. Posteriormente, se trataron seis grupos con diferentes sustancias de ensayo y se analizaron cualitativa y cuantitativamente a los 30 y 60 días. Las p ruebas utilizadas fueron Kruskal - Wallis y Dunn's. Shapiro - Wilk y ANOVA. El análisis cualitativo a los 30 días mostró una diferencia entre los grupos tratados con extracto etanólico y pasta dentífrica. Cuantitativamente, a los 30 días, el tratamiento con ex tracto etanólico de Casearia mostró un mayor número de túbulos dentinarios abiertos. A los 60 días, la diferencia persistió sólo para los bloques tratados con pasta dentífrica. Los resultados obtenidos indicaron que existe una relación positiva entre el us o de Casearia sylvestris y la obliteración de los túbulos dentinarios
Subject(s)
Animals , Cattle , Casearia/chemistry , Dentin Desensitizing Agents/therapeutic use , Plant Extracts/analysis , Dentifrices/therapeutic useABSTRACT
Determination of the mineral composition is important for further study and standardization of the dried branchesof the species from the Salix (willow) family genus and extracts from the plant material. The study aimed todetermine the qualitative and quantitative content of macro- and microelements in some representatives of theSalix spp. growing in Ukraine in comparison with soils and extracts of plant material. The elemental compositionof the branches of Salix cinerea L., S. incana Schrank, S. cаprea L., S. sachalinensis F. Schmidt, S. acutifolia L.,S. fragilis L., S. caspica Pall., S. rosmarinifolia L., and S. myrsinifolia Salisb was determined using atomicemission spectrometry with photographic registration for the first time. The method used allowed us to determinethe presence of 5 macro- (К, Na, Ca, Р, Mg) and 14 microelements (Fe, Si, Мn, Al, Sr, Zn, Ni, Mo, Cu, Co, Pb,Cd, As, Hg), and the content of 14 elements were quantified in the dried branches of the Salix spp. plants. Theability to accumulate and a relatively high content of micro- and macroelements, as well as low content of toxicmicroelements (Co, Pb, Cd, As, Hg) in willow branches, allow considering them as a promising but safe sourceof biologically available microelements. There were typically not correlations between concentrations of microand macroelements in plant material and soils. The maximum levels of macroelements and microelements werefound in Salix species collected in the Kharkiv region, and in the Zakarpatye region, respectively. The elementalcomposition in the Salix sachalinensis F. Schmidt was quite different if compared with all others.