Analysis of Various Histopathological Patterns in Pleomorphic Adenoma - An Institutional Case Study

Introduction: Pleomorphic adenoma, the most common benign mixed salivary gland tumor which accounts for 70-80%, is a biphasic tumor with dual origin from epithelial and myoepithelial elements. It shows a malignant transformation rate of 6%. Microscopically, pleomorphic adenoma characteristically displays vast morphologic diversity. This present study is done to analyze prevalence rates and histomorphological variations to better elucidate the pattern of occurrence, diagnosis and treat- ment plan of pleomorphic adenoma. Materials and Methods: This are a retrospective study which was conducted in a private institution, Chennai. Based on the proportion of parenchymal and stromal tumor components, the cases were classified into four subtypes as proposed by Foote and Frazell. Morphological patterns, cellular patterns, capsular alterations and Stromal components were analyzed and statisti- cally evaluated. Results: Our analysis showed an overall 0.87% of pleomorphic adenoma cases being reported were associated with minor salivary glands followed buccal mucosa and palate. All the reported cases showed Type II pattern with histological cellular pat- tern of spindle cells and ductal morphological pattern. Conclusion: We observed the great diversity of morphological aspects of the stroma in pleomorphic adenoma, in which many cases showed variation in morphology and cellularity associated with tumor location.

AN ANALYSIS OF CYTOLOGICAL SPECTRUM OF SALIVARY GLAND LESIONS IN A TERTIARY CARE HOSPITAL IN SOUTH INDIA

Introduction: Salivary gland tumors are uncommon neoplasm of head and neck tumors. FNAC can provide cytological categorization of salivary gland lesions for guiding the surgeons to make treatment decisions. This study is intended to analyze the cytological spectrum of salivary gland lesions in Aims and Objectives: correlation with age, gender, and site of the lesion. A total of 222 cases are included in this study spanning a period Methods: from January 2015 to December 2018. The clinical data pertaining to patients' age, sex and anatomical site were recorded. Cytological smears were reviewed. The clinical features, imaging ?ndings, cytopathology and histopathology ?ndings were analyzed. In this three year study period, out of 222 cases, 129 cases were non-neoplastic and 93 cases were Results: neoplastic. Commonest gland involved was parotid gland followed by submandibular gland. Malignant lesions accounted for about 17.2% of the neoplastic lesions. In our study, majority of lesions are non-neoplastic. Among them Conclusion: sialadenitis was the most commonly encountered lesion. Among benign neoplasm, pleomorphic adenoma was the most common neoplasm with slight female preponderance. Among malignant tumors, mucoepidermoid carcinoma was the most common malignant tumor

Histopathological study of salivary gland tumors: a tertiary care hospital-based study

Introduction: Salivary gland tumors (SGTs) are relatively uncommon and show a wide variety of morphological heterogeneities. The incidence of SGTs is said to be affected by geographical and racial factors. Histopathological diagnosis plays a major role in the diagnosis of these neoplasms. Objectives: This study investigated the incidence of SGTs and histopathological features of various SGTs, aimed at knowing the epidemiological pattern of these tumors and comparing the results of our study with other studies of SGTs in the literature. Materials and Methods: A retrospective study was conducted on SGTs at the Pathology Department of the Government Medical College and Hospital for a period of 5 years. Clinical and demographic data were documented and analyzed concerning histopathologic type. Results: A total of 86 SGTs were identified, 65.12% of which were classified as benign and 34.88% as malignant. Most tumors occurred in the parotid gland (59.14%). Pleomorphic adenoma (PA) was the most common tumor (56.98%), followed by mucoepidermoid carcinoma (MEC) (17.44%). The tumors occurred more often in men (51.16%) than in women (48.84%). Conclusion: SGTs exhibit broad morphological heterogeneity and these tumors are rare. The parotid gland is the most common location, and PA is the most frequent benign tumor. MEC followed by adenoid cystic carcinoma is the most common malignancy of the salivary gland.

Pleomorpic Adenoma of Infratemporal Fossa Co- Existing with Actinomycosis: A Rare Case Report with Review of Literature

Introduction: Salivary gland tumors account for about 3% of head and neck tumors and the majority are benign in nature. Among these, the most common pathological type is pleomorphic adenoma also called the mixed tumor. It is a kind of tumor containing glandular tissue, myxoid and cartilage-like tissue. As the structure of the tissue is diverse, it is called a “mixed tumor.” Actinomycosis is a chronic suppurative bacterial infection caused by Actinomyces israelii. In this case report, we are presenting a rare co-existence of pleomorphic adenoma and Actinomycosis in the infratemporal fossa. Case description: A 40-year-old female patient complains of pain over the upper left back tooth region for the past 6months. The patient had a past history of a small lesion in 28 region which resulted in constant irritation. The patient had consulted the dentist for the same and underwent extraction of 28 and 38 three years back, which was thought to be the cause of the swell- ing. Conclusion: The case highlights the importance of proper history taking, clinical examination, and histopathological examina- tion in arriving at a precise diagnosis of a lesion irrespective of size and clinical presentation.

Inverted ductal papilloma of the salivary gland

@#This is a case consult of slides stated to be from an excision of a buccal mucosa mass in a 58-year-old-man. The specimen was described as a 3 cm diameter roughly oval tan-gray tissue with a 2 x 1.5 cm mucosal ellipse on the surface that has a central ulcerated punctum. Cut section showed an underlying 1.7 cm diameter roughly oval well-circumscribed mass with a granular tan surface. Histological sections show a papillary lesion with an orifice on the mucosal surface and with epithelial nests invaginating into the underlying lamina propria in a non-infiltrative pattern. (Figure 1) The lesion is composed of papillary epithelial fronds with cleft-like spaces between the fronds. (Figure 2) The papillary fronds are lined by non-keratinizing basaloid stratified squamous cells with a superficial layer of columnar glandular cells along with mucous goblet cells interspersed among the squamous cells. (Figure 3) All the cellular components are devoid of cytologic atypia and mitoses. Based on these microscopic features we signed the case out as inverted ductal papilloma (IDP).

Histomorphological study of salivary gland neoplasms: fifteen year study

Background: The neoplasms of salivary glands are relatively uncommon and represent less than 2% of all human tumors. They are morphologically diverse, with marked heterogeneity among the different subtypes and even within the same tumor. The unpredictability in the long term outcome imposes a significant challenge in the clinical management.Methods: This is a descriptive study done in a tertiary care teaching hospital over a period of 15 years. Patient details were collected from medical case records. All specimens were evaluated for site, laterality, size, nature of the cut surface and intactness of the capsule. Two to 5 representative bits were taken from each specimen and subjected to routine fixation, processing and section cutting followed by Haematoxylin and Eosin staining.Results: A total of 138 salivary gland tumors were diagnosed from patients in the age group of 13 to 90 years with a mean age of 41.8 years. There were 53 (38.4%) males and 85 (61.6%) females with male to female ratio of 1:1.42. There were 115 (83.3%) benign tumors and 23 (16.7%) malignant tumors. Pleomorphic adenoma accounted for 70.3% of all salivary gland tumors followed by mucoepidermoid carcinoma (10.9%), Warthin tumor (8%), basal cell adenoma (2.9%), adenoid cystic carcinoma (2.2%), squamous cell carcinoma (2.2%), myoepithelioma (1.4%), oxyphillic adenoma (0.7%), acinic cell carcinoma (0.7%) and salivary duct carcinoma (0.7%).Conclusions: The salivary gland neoplasms are relatively uncommon head and neck tumors. Parotid gland is the commonest site of occurrence. Pleomorphic adenoma is the commonest benign and mucoepidermoid carcinoma the commonest malignant salivary gland tumor.

Fine needle aspiration cytology of minor salivary gland tumors: A retrospective 5-year study of 42 cases in a tertiary care hospital

Background: Minor salivary gland tumors (MSGTs) are less common than major salivary glands and involve only 15–20% of all salivary gland tumors. Most of the cases originate at intra- and peri-oral region. Minor salivary gland lesion cytology has been studied rarely in India. Aims: This present study was performed to evaluate the role of fine needle aspiration cytology (FNAC) in diagnosis of MSGTs and to explore the cases of cytohistological discrepancies in the study. Materials and Methods: This retrospective study was conducted over a 5-year period on 42 cases of MSGTs. In all the cases, cytology was correlated with histology and cytohistological discrepancies were searched. Sensitivity, specificity, and diagnostic efficacy were calculated using histopathology as gold standard. Results: We diagnosed 27 malignant (64.28%) and 15 benign (35.71%) MSGTs in cytological evaluation. We found two false negative and one false positive case in cytology. Sensitivity, specificity, and diagnostic accuracy of the study were 92.59%, 93.33%, and 92.85%, respectively. Conclusion: FNAC is a minimally invasive and cost-effective procedure with high accuracy (92.85%) in the assessment of MSGTs and helps in the management of benign and malignant tumors.

Salivary Gland Tumors - A Three Year Retrospective Study

Neoplastic lesions of salivary gland are uncommon and shows varied morphology. It comprises 5-6 % of all tumors of head and neck. Salivary neoplasms are more likely to occur in relatively old age (6th decade). Benign salivary gland tumors usually occur relatively earlier (4th-5th decade) than malignant tumors. Tumors of salivary glands usually present as mass in otherwise normal gland. Uncommon presenting complaints such as facial paralysis in presence of neoplastic growth usually suggest malignant tumor invading facial nerve. Our aim is to study the epidemiology, clinical presentation and distribution of various salivary glands in relation to their histopathology. Methods: This was a retrospective cross-sectional study conducted in the department of pathology of a tertiary care medical centre situated in an urban area. All the salivary gland tumor specimens which were received in the department over these years were studied by analyzing records from the department. Distribution of benign and malignant salivary gland tumors, their morphology and prognostic histological parameters were studied. Statistical analysis was done using SSPS 17.0 software, with P value being less than 0.05 as statistically significant. Results: Out of 80 studied cases there were 32 males and 48 females with a M:F ratio of 1:1.5. The mean age in male and female patients was found to be 47.41 ± 15.18 and 46.72 ± 13.68 respectively. The difference in mean age of males and females was found to be statistically insignificant (P>0.05). parotid gland was most commonly involved site (68.75%) followed by submandibular gland (23.75%) and minor salivary glands (5%). Amongst benign pathologies pleomorphic adenoma was found to be the most common lesion encountered(31.25%) followed by warthin’s tumor (11.25%) and myoepithelioma (5%). The most common malignant tumor was mucoepidermoid carcinoma which was seen in 12 (17.50%) patients. Conclusion: Amongst all salivary glands, parotid gland was found to be most commonly affected. Most common benign and malignant tumors were found to be pleomorphic adenoma and mucoepidermoid carcinoma respectively.

Clinicopathological Study Of Salivary Gland Tumors: A Three Years Prospective Observational Study

Background: The salivary glands are one of the few tissues in the body that are subjected to diverse and heterogeneous range of tumors and tumor like conditions. The relative infrequency of these tumors makes their diagnosis and management quite complicated. Fine needle aspiration cytology (FNAC) is a useful diagnostic procedure which has a recognized role in the evaluation of salivary gland lesions. A pre-operative diagnosis about nature of lesion, whether benign or malignant, will help in making decision about proper management of patient. Aim and Objective: Aim of this study was to know the epidemiology of salivary gland tumors in our region and to evaluate sensitivity, specificity and diagnostic accuracy of fine needle aspiration cytology taking histopathology as the gold standard. Material and Method: It was 3yrs prospective observational study conducted from oct 2015 to oct 2018 in department of pathology KMC/MGM Hospital. Total 59 cases were studied with particular reference to age, sex, site, cytologic details & histological types as per WHO classification. FNAC & histopathological examination was done in all cases came to pathology laboratory . Correlation between cytological & histopathological diagnoses was assessed. Diagnostic accuracy of FNAC was evaluated by comparing cytological & histopathological diagnoses. Results and Discussion : Parotid gland was the most common site of involvement (70% cases). Maximum number of patients was in age range of 41-50 years & male to female ratio was 0.8:1. Most common benign & malignant tumors were pleomorphic adenoma (69.89% cases) & mucoepidermoid carcinoma (8.60% cases) respectively. On cytology, 54 cases while on histopathology 57 cases were diagnosed as neoplastic. Cytological diagnoses correlated with histopathological diagnoses in 94% cases. Sensitivity, specificity & diagnostic accuracy of FNAC were found to be 96.87%, 100% & 96% respectively. Conclusion: FNAC in salivary gland masses is fairly reliable for correct preoperative diagnosis. Multiple sampling and special attention to cytologic features should help to minimize errors

Palatal Pleomorphic Adenoma in a Pediatric Patient: Case Report / Adenoma Pleomorfo Palatino en un Paciente Pediátrico: Reporte de Caso

Pleomorphic adenoma is the most common benign neoplasm of salivary glands. Their common location is in parotid gland, however, a lower percentage of these tumors might occur in minor glands. The epidemiology of this tumor shows that adults are the most affected, with rare occurrence in children or adolescents. We present the case report of pleomorphic adenoma located on the palate of a 10 year old. Excisional biopsy of the lesion followed by histopathologic examination of the biopsy specimen revealed ductal structures surrounded by plasmacytoid mioepithelial cells within a myxoid stroma, the final diagnosis corresponded to Pleomorphic Adenoma. Early detection and excision of this lesion in children are important to minimize potential recurrences or malignant transformation.

El adenoma pleomorfo es la neoplasia benigna más común de las glándulas salivales. Su localización común está en glándula parótida, sin embargo, un bajo porcentaje de estos tumores puede ocurrir en glándulas menores. La epidemiología de este tumor muestra que los adultos son los más afectados, con rara ocurrencia en niños o adolescentes. Presentamos el caso de un adenoma pleomorfo localizado en el paladar de un niño de 10 años. La biopsia excisional de la lesión seguida de examen histopatológico de la muestra de biopsia reveló estructuras ductales rodeadas por células mioepiteliales plasmocitóides dentro de un estroma mixoide, siendo el diagnóstico final adenoma pleomorfo. La detección temprana y la excisión de esta lesión en los niños es importante para minimizar las recidivas potenciales o la transformación maligna.

Study of salivary gland neoplasms in pediatric age group in a tertiary care centre for head and neck diseases

Introduction: Salivary gland tumors are very rare in children and adolescents. We reported here 11 cases of paediatric salivary gland tumors in a study conducted from July 2015 to June 2017 at Government ENT Hospital, Koti, Hyderabad, Telangana, a tertiary care center. Objective: The aim of this study was to analyze clinical and histopathological features of salivary gland tumours in paediatric age group (children and adolescents). Materials and methods: A total of 11 cases of salivary gland tumors encountered in paediatric age group (0-18 years) were studied and analyzed for age, gender, site predilection and for histopathological types. Results: Parotid and submandibular glands were the main site of tumor occurrence (8/11 in parotid gland, 2/11 in submandibular gland and 1/11 arising in minor salivary glands of hard palate). 4 cases were benign tumors (pleomorphic adenoma) and 7 were malignant tumors (constituted by 6 cases of low grade mucoepidermoid carcinomas and a single case of adenoid cystic carcinoma). Majority of the cases were above 10 years of age with an average of 12.3 years with female preponderence. Conclusion: Present study showed that salivary gland tumors in paediatric age group are very rare and with majority of cases being Mucoepidermoid carcinoma with female preponderance seen in parotid gland. Other glands involved were submandibular salivary glands and minor salivary glands of hard palate. Pleomorphic adenoma was the most common benign tumor and Mucoepidermoid carcinoma was the most common malignant tumor, all of low grade type. One case of adenoid cystic carcinoma was noted in a 15 year old female.

Clinicopathological study of salivary gland tumors: An observation in tertiary hospital of central India.

Background: The salivary glands are one of the few tissues in the body that are subjected to diverse and heterogeneous range of tumors and tumor like conditions. The relative infrequency of these tumors makes their diagnosis and management quite complicated. Fine needle aspiration cytology (FNAC) is a useful diagnostic procedure which has a recognized role in the evaluation of salivary gland lesions. A pre-operative diagnosis about nature of lesion, whether benign or malignant, will help in making decision about proper management of patient. Objective: Aim of this study was to know the epidemiology of salivary gland tumors in our region and to evaluate sensitivity, specificity and diagnostic accuracy of fine needle aspiration cytology taking histopathology as the gold standard. Method: It was prospective observational study conducted in department of pathology in our institute. Total 100 cases were studied with particular reference to age, sex, site, cytologic details & histological types as per WHO classification. FNAC & histopathological examination was done in all cases. Correlation between cytological & histopathological diagnoses was assessed. Diagnostic accuracy of FNAC was evaluated by comparing cytological & histopathological diagnoses. Results: Parotid gland was the most common site of involvement (70% cases). Maximum number of patients was in age range of 41-50 years & male to female ratio was 0.8:1. Most common benign & malignant tumors were pleomorphic adenoma (69.89% cases) & mucoepidermoid carcinoma (8.60% cases) respectively. On cytology, 93 cases while on histopathology 96 cases were diagnosed as neoplastic. Cytological diagnoses correlated with histopathological diagnoses in 94% cases. Sensitivity, specificity & diagnostic accuracy of FNAC were found to be 96.87%, 100% & 96% respectively. Conclusion: FNAC in salivary gland masses is fairly reliable for correct preoperative diagnosis. Multiple sampling and special attention to cytologic features should help to minimize errors.

MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2)

Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors. Objective The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue. Material and Methods Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used. Results Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive. Conclusions Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts. .

Oncocytoma of parotid gland: A rare case report.

Oncocytomas are benign salivary gland tumors composed of oncocytes, cuboidal to columnar epithelial cells with abundant eosinophilic cytoplasm secondary to the accumulation of excessive number of mitochondria. In 1894, the German pathologist Hurthle first described these granular cells in normal canine thyroid glands, while the term "Oncocyte" was coined by Hamperl in 1931. Fine Needle Aspiration Cytology (FNAC) has increasingly been used as a primary screening tool for salivary gland lesions with high levels of sensitivity and specificity. However, caution should be exercised when interpreting aspirates with predominant oncocytic population. Final diagnosis is assisted by CT and/or magnetic resonance imagine (MRI) of the neck and histopathologic examination.

Expression of maspin in benign and malignant salivary gland tumors: An immunohistochemical study.

Context: Maspin is a novel serine protease inhibitor (serpin) with multifaceted tumor‑suppressive activities. It was originally identified in normal human breast myoepithelial cells and shows variable expression in different types of cancer cells. Maspin displays anti‑metastatic properties in mammary and prostate cancer. Its expression is maintained during ovarian, lung and pancreatic carcinogenesis, indicating that Maspin regulated metastatic potential is tissue specific. Thus, it is possible that Maspin participates in salivary gland tumor biology as well. In this study, expression pattern of maspin in benign and malignant salivary gland tumors is analyzed, to understand the biological behavior of salivary gland tumors with respect to maspin expression. Aims and Objectives: The aim of this study was to demonstrate, record, and correlate the expression pattern of maspin in benign and malignant salivary gland tumors. Settings and Design: A retrospective study of maspin expression in 30 diagnosed cases of benign and malignant salivary gland tumors retrieved from archives of our department. Materials and Methods: Anti‑maspin antibody and horseradish peroxidase detection system. Statistical Analysis: Descriptive statistical analysis and Chi‑square/Fisher Exact test. Results: Intense expression with P < 0.001 is associated with benign tumors, nuclear staining with P < 0.001 is significantly associated with benign tumors and cytoplasmic staining with P = 0.020 is associated with malignant tumors. Conclusion: Intensity of expression is more in benign tumors when compared with malignant tumors. The benign tumors showed both nuclear and cytoplasmic expression. Some malignant tumors did express maspin, but mainly in the cytoplasm.

Punción con aguja fina en tumores de glándulas salivales / Fine needle aspiration in salivary gland tumors

Los estudios por imagen permiten diagnosticar y categorizar un tumor de glándula salival (GS). La punción con aguja fina (PAAF) es una herramienta diagnóstica. El ultrasonido (US) permite visualizar el tumor y guiar la aguja de PAAF. Objetivos: Describir la técnica de PAAF. Revisión retrospectiva de los resultados de PAAF bajo US en tumor de GS. Se analizaron 39 pacientes enviados a PAAF en 5 años. Resultados: 31 lesiones parotídeas y 8 submaxilares. 87 por ciento se presentaron como nódulo hipoecogénico y 69 por ciento con refuerzo posterior. Resultado cito-histológico de 36 PAAF realizadas: 29 lesiones benignas, 6 lesiones malignas, 1 punción frustra. No hubo complicaciones. 21 pacientes operados. Concordancia histológica 85 por ciento . No hubo falsos positivos para cáncer. En conclusión, PAAF bajo US es una herramienta diagnóstica en los tumores de GS.

Imaging studies allow the diagnosis and classification of a salivary gland (SG) tumor. Fine needle aspiration (FNA) is a diagnostic tool. Ultrasound (US) allows the visualization of the tumor and guidance of the needle in FNA. Objectives: To describe the FNA technique. A retrospective review of the results of ultrasound guided FNA in a SG tumor. 39 patients referred for FNA in 5 years, were analyzed. Results: 31 parotid and 8 submaxillary lesions. 87% presented as hypoechoic nodule and 69% with posterior acoustic enhancement. Cyto-histological results of 36 FNA performed: 29 benign lesions, 6 malignant lesions, one failed puncture. There were no complications. 21 operated patients. Histological concordance was 85%. There were no false positives for cancer. In conclusion, FNA using ultrasound is a diagnostic tool in GS tumors.

A histopathologic study of salivary gland tumors in Kano, northern Nigeria.

Significant differences in the global distribution of salivary gland tumors have been reported, but no formal study has been carried out here in Kano, the largest city in northern Nigeria. We therefore undertook this eight-year retrospective study of all histologically diagnosed salivary neoplasms at the histopathology laboratory of our referral teaching hospital in Kano. Seventy-eight salivary gland tumors were diagnosed during the eight-year study period accounting for 0.4% of all neoplasms. Benign tumors were more prevalent, comprising 56.4%, while malignancies were 43.6%. Pleomorphic adenoma and mucoepidermoid carcinoma were the commonest histological types constituting 48.7% and 23.1% respectively, while the most frequent sites were parotid, submandibular and minor salivary glands accounting for 49%, 26% and 24% respectively. As in most studies of black populations there were no adenolymphomas. Age distribution was bimodal with a benign peak in the third decade and a malignant peak in the sixth. Our findings were broadly similar to most other African reports but somewhat at variance with Western literature. Improved hospital attendance with more comprehensive reporting would yield more representative data.

Expression of the genes of vascular endothelial growth factor and von willebrand factor in salivary gland tumors

Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors

Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.

As células mioepiteliais apresentam um imunofenótipo complexo, variando a expressão de suas proteínas na dependência do seu estágio de diferenciação normal ou neoplásico. Com o objetivo de avaliar comparativamente marcadores imuno-histoquímicos para estas células, um painel de anticorpos composto pela vimentina, calponina e HHF-35 foi aplicado em 28 tumores de glândulas salivares. Os resultados demonstraram que a vimentina e a calponina foram percentualmente mais sensíveis que o HHF-35; entretanto, a calponina e o HHF-35 apresentaram padrão de distribuição focal diferentemente da distribuição difusa da vimentina. As células predominantemente marcadas, por todos os anticorpos utilizados, foram as não luminais presentes nas estruturas ductiformes e tubulares, vistas no adenoma pleomórfico e no carcinoma adenóide cístico, bem como as células dos cordões e ninhos dos adenocarcinomas polimorfo de baixo grau e periferia de lençóis e ninhos dos mioepiteliomas. Em conclusão, sugere-se que se faça associação da calponina com vimentina para identificação de células mioepiteliais em neoplasias de glândula salivar.