Deficiency of rpoS is the major factor leading to attenuation of Salmonella enterica serovar Choleraesuis vaccine strain C500 / 中国人兽共患病学报

Salmonella enterica serovar Choleraesuis strain C500 is a live attenuated vaccine that has been widely used in Chi‐na for over 50 years to prevent piglet paratyphoid .However ,as C500 is obtained by chemical methods ,the genetic background of this strain remained unclear .In this study ,we compared the genomic differences between the virulent reference strain C 78‐2 and C500 by suppression subtractive hybridization combined with the mirror orientation selection method (MOS‐SSH ) .Six genes (asr ,ydgF ,ydgD ,ydgE ,rpoS ,and ptsG) were lost in C500 strain .Using real‐time PCR analysis ,we demonstrated that the genes regulated by rpoS ,a vital transcriptional regulator playing an important role in Salmonella infection ,were downregulated in C500 .Additionally ,the virulence of the rpoS mutant strain C78‐2ΔrpoS was 100 000 times lower than the parental strain in BALB/c mice .So loss of rpoS gene is the major factor leading to the attenuation of C500 strain .

One Case of Thoracic Empyema due to Salmonella choleraesuis / 감염과화학요법

Salmonella are motile, gram-negative, non-spore-forming members of the family Enterobacteriaceae. Among nontyphoid Salmonella serotypes, Salmonella choleraesuis shows a high predilection to cause systemic infections in humans. Thoracic infection is a rare complication of Salmonella infection. So far, most of reported cases of empyema caused by Salmonella spp. have involved immunocompromised patients. Herein, as we had experienced one case of thoracic empyema due to Salmonella choleraesuis related thymoma, we report it with review of literature.

One Case of Thoracic Empyema due to Salmonella choleraesuis / 감염과화학요법

Salmonella are motile, gram-negative, non-spore-forming members of the family Enterobacteriaceae. Among nontyphoid Salmonella serotypes, Salmonella choleraesuis shows a high predilection to cause systemic infections in humans. Thoracic infection is a rare complication of Salmonella infection. So far, most of reported cases of empyema caused by Salmonella spp. have involved immunocompromised patients. Herein, as we had experienced one case of thoracic empyema due to Salmonella choleraesuis related thymoma, we report it with review of literature.

Septicemia por salmonella no tiphy / Salmonella non tiphy septicemia

Se trata de un paciente con tos de 5 meses de evolución, fiebre de 3 meses, que ha visto otros servicios de salud para solucionar su problema. Con investigaciones negativas para tuberculosis, un cultivo de esputo con klebsiella Pneumoniae por lo que recibió ciprofloxacina durante 5 días. Viene al hospital Vozandes Quito, aún con fiebre, malestar genera y odinofagia. En el examen físico un eritema leve en orofaringe y un ganglio cervical, el resto normal. Es hospitalizado como fiebre de origen desconocido y se inicia investigaciones básicas que inicialmente destacan alteración en reactantes de fase aguda y hepatopatía. Con exámenes más invasivos se dermina por un mielocultivo la presencia de salmonella cholerasuis. Luego de 4 días de 2 gramos diarios de ceftriaxona intravenosa cede la fiebre. A los 7 días de antibióticoterapia sistémica, sale con el alta y toma ciprofloxacina 750 mg dos veces al día por 10 días más. Presentamos dos revisiones bibliográficas sobre fiebre de origen a determinar y salmonellosis por choleraesuis. En el Ecuador se han reportado pocos casos de infección por salmonella cholerasuis, en un estudio realizado en 1989 y 1993 entre todas las especies de salmonelas el 36% eran de serotipo Cholerasuis, Se ha aislado de tejidos de pollo, a diferencia de Asia donde es frecuente que habite en cerdos. Se transmite a través agua y alimentos contaminados. Se caracteriza por dos tipos de presentación clínica: Síndrome e bacteriemia sostenida con fiebre e infecciones focales extraintestinales. Todavía no hemos encontrado resistencia antibiótica, como se ha reportado en otros países.

This is a patient with a cough of 5 months of evolution, fever of 3 months, who has seen other health services to solve his problem. With negative investigations for tuberculosis, a sputum culture with klebsiella Pneumoniae so he received ciprofloxacin for 5 days. He comes to the Vozandes Quito hospital, even with fever, general discomfort and odynophagia. On physical examination, a slight erythema in the oropharynx and a cervical ganglion, the rest normal. He is hospitalized as a fever of unknown origin and basic investigations are started that initially highlight alteration in acute phase reactants and liver disease. With more invasive examinations, the presence of salmonella cholerasuis is revealed by a myeloculture. After 4 days of 2 grams daily of intravenous ceftriaxone fever subsides. After 7 days of systemic antibiotic therapy, she is discharged and takes ciprofloxacin 750 mg twice a day for 10 more days. We present two bibliographic reviews on fever of origin to be determined and choleraesuis salmonellosis. In Ecuador, few cases of salmonella cholerasuis infection have been reported. In a study carried out in 1989 and 1993, 36% of all salmonella species were of the Cholerasuis serotype. They have been isolated from chicken tissues, unlike Asia, where it is frequently inhabits pigs. It is transmitted through contaminated food and water. It is characterized by two types of clinical presentation: syndrome and sustained bacteremia with fever and focal extra-intestinal infections. We have not yet found antibiotic resistance, as has been reported in other countries.

Salmonella choleraesuis bacteremia associated with The Acquired Immunodeficiency Syndrome (AIDS).

Multiple opportunistic infections are characteristic of the acquired immunodeficiency syndrome (AIDS). An emerging problem with salmonella infection among patients with AIDS is increasing. We report here 3 patients with AIDS who presented with fever and were found to be bacteremia due to serotypes of Salmonella choleraesuis. As this serotype rarely occurs in normal hosts in Thialand, finding the infection in patients with AIDS indicate that these patients are at high risk. It suggests that AIDS patients with prolonged febrile illness should be investigated for salmonella infection.