ABSTRACT
Objective:To develop an HPLC method for the simultaneous determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins. Methods:The column was Agilent-C18 (250 mm × 4. 6 mm,5μm) with the temperature of 25℃, the mobile phase was methanol-water-glacial acetic acid-triethylamine (90 ∶10 ∶0. 03∶0. 06) at the flow rate of 1. 0 ml·min-1 ,and the detection wavelength was 210 nm. Results: The calibration curves were linear within the range of 7. 50-75. 00 μg · ml-1 for oleanic acid(r=0. 999 7)and 5. 00-50. 00 μg · ml-1 for ursolic acid(r=0. 999 7). The average recovery of oleanic acid and ursolic acid was 100. 15%(RSD=1. 14%,n=6)and 99. 40%(RSD=1. 67%,n=6), respectively. Conclusion: The method is simple and accurate, which is suitable for the determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins.
ABSTRACT
Objective: To study the chemical constituents from the herb of Salvia plebeian. Methods: The constituents were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and preparative TLC. The structures of the isolated compounds were elucidated by spectroscopic analysis and physicochemical properties. Results: Eleven compounds were isolated and purified from the ethanol extract of S. plebeia. They were identified as 5,6-dihydroxy-7,4'-dimethoxyflavone (1), 5,6,3'-trihydroxy-7,4'-dimethoxyflavone (2), nepetin (3), homoplantaginin (4), apigenin (5), cirsiliol (6), 6-methoxynaringenin (7), emodin (8), maslinic acid (9), isorosmanol (10), and (-)-epiloliolide (11). Conclusion: Among them, compound 11 is isolated from the plants of Salvia L. firstly, and compounds 1, 2, 6, 9, and 10 are isolated from S. plebeian for the first time.