Constituents from the Branches of Sambucus sieboldiana var. pendula with the Properties of Collagen Synthesis Activation.

For the purpose of developing anti-wrinkle cosmetic ingredients, the extracts from branches of a woody plant Sambucus sieboldiana var. pendula were examined on collagen synthesis activities using fibroblast HDFn cells. As a result, the S. sieboldiana ethanol extract (SSE) proved to activate the production of type I procollagen in a dose-dependent manner without showing cell toxicity. Phytochemical study was conducted to isolate the active constituents in the extracts by solvent fractionation followed by chromatographic purifications. From this procedure, two known compounds, kaempferol 3-O-sophoroside (1) and daucosterol (2), were identified by spectroscopic studies. From the isolates, the flavonoid glycoside 1 was verified to induce the synthesis of the type I procollagen dose-dependently. These results suggested that S. sieboldiana extract containing the flavonoid 1 could be useful as an active ingredient in wrinkle-care cosmetics.

Effect of Sambucus sieboldiana Extract on the Cell Growth and Extracellular Matrix Formation in Osteoblast Cells

Sambucus sieboldiana (SS) is a member of the family Caprifoliaceae and has been recommended as a functional material because of its several bioactivities. Although numerous literatures are available on the pharmacological and biological activities, the biological activity of SS in bone regeneration process has not yet been well-defined. Therefore, in this study, the effect of SS was investigated in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. The treatment of SS did not significantly affect the cell proliferation in MC3T3-E1 cells. SS significantly accelerated the mineralization and significantly increased the expression of alkaline phosphatase (ALP) and osteocalcin (OC) mRNAs, compared to the control, in the differentiation of MC3T3-E1 cells. SS significantly accelerated the decrease of osteonectin (ON) mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. These results suggest that the SS facilitate the osteoblast differentiation and mineralization in MC3T3-E1 osteoblastic cells. Therefore, there may be potential properties for development and clinical application of bone regeneration materials.