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AIM: To observe protective effects of sanguis draxonis flavones on myocardial ischemia in rats and dogs. METHODS: Acute myocardial ischemia in rats was produced by iv pituitrin and ECG indexes were observed. Myocardial ischemia was induced by ligating coronary artery in anaesthetized dogs, and then EEC, CK,LDH, and LD in serum were determined respectively. RESULTS: J point and T wave in rats changed evidently after iv pituitrin, which was reversed by sanguis draxonis flavones (360, 180 mg?kg~ -1). In coronary artery ligation model, infraction range, △N-ST, △?-ST and some serum indexes (such as CK, LDH and LD) was decreased after ig sanguis draxonis flavones (120, 60, 30 mg?kg~ -1). CONCLUSION: Acute myocardial ischemia is protected effectively by sanguis draxonis flavones.
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Object To study the active constituents of Sanguis Draxonis made form Dracaena cochinchinensis (Lour ) S C Chen in China * Methods Various column chromatographies with Sephadex L 20 gel, MCI gel and silica gel were employed for the isolation and purification The structures of compounds were elucidated by spectral analysis Results Twelve compounds were isolated from the commercial product available on the market By means of spectral data, they were identified as 26 O ? D glucopyranosyl furostan 5,25 (27) diene 1?,3?,22?,26 tetrahydroxy 1 O ? L arabinopyranoside (Ⅰ); 3, 4 dihydroxy allylbenzene 4 O ? D glucopyranoside (Ⅱ); 7 hydroxy 3 (p hydroxyphenyl) chroman (Ⅲ); 7, 4′ dihydroxy 3′ methoxyflavan (Ⅳ); 3, 4 dihydroxyallylbenzene (Ⅴ); resveratrol (Ⅵ); 7, 4′ dihydroxy flavanone (Ⅶ); di (p hydroxyphenyl) methane (Ⅷ); acanthoside B (Ⅸ),p hydroxybenzoic acid (Ⅹ); hydroquinone (Ⅺ) and protocatechualdehyde ( ⅩⅡ ) Conclusion Compound Ⅰ and Ⅱ are new natural glycosides, and Ⅴ and Ⅵ are isolated from D cochinchinensis for the first time
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Objective: To establish HPLC method for the determination of loureirin A and loureirin B in Sanguis Draxonis Capsules.Methods: HPLC system included C 18 reverse phase column and acetonitrile 1% acetic acid (34∶66) as mobile phase, detection at 280nm and external standard method.Results:The standard curves of loureirin A was linear in the concentration range of 98～490ng, r =0.9996.The average recovery was 97.45%, RSD was 1.82%.The standard curves of loureirin B was linear in the concentration range of 43.2～259.2, r = 0.9993 .The average recovery was 96.92%, RSD was 1.57%.The contents of loureirin A and loureirin B in Sanguis Draxonis from heating free technology were higher than traditional technology.Conclusion: This heating free technology is a new technique that is a creation with higher extract rate,this method is worth populariging.