ABSTRACT
Objective: To establish the regeneration system of Saposhnikovia divaricate (Turcz.) Schisck. Methods: The effects of different explants, temperature, medium, and illumination was studied on the induction of S. divaricate. Response surface test design was used to optimize the hormone ratio of callus induction and proliferation. Results: The leaves of S. divaricate were used as the experimental materials, the optimal medium for the callus induction was MS + 6-BA 0.90 mg/L + 2,4-D 1.05 mg/L + KT 0.96 mg/L, with temperature of 25 ℃ and light of 12 h/d. The optimal medium for callus proliferation was MS + 6-BA 0.56 mg/L + 2,4-D 0.48 mg/L + KT 0.40 mg/L, and the subculture cycle was 35 d. The optimal medium for adventitious shoots was MS + NAA 0.6 mg/L + 6-BA 1.0 mg/L. The optimal medium for root growing was MS + NAA 0.4 mg/L + 6-BA 0.1 mg/L. Conclusion: S. divaricate callus was successfully induced and a stable and efficient plant regeneration system was established.
ABSTRACT
Saposhnikovia divaricate, a kind of traditional medicinal herbs, is widely used in China. The chemical composition of S. divaricate is very complicated, whose main active substances are ketone, coumarin, volatile oil and other ingredients. The pharmacological activities of S. divaricate are mainly concentrated in the antipyretic, analgesic, anti-inflammatory, and other aspects. In this paper, the literatures on the chemical composition and pharmacological effects of S. divaricate at home and abroad are summarized, and some suggestions are put forward for future research. The aim is to provide theoretical reference for the development and utilization and in-depth study of S. divaricate.