ABSTRACT
Objective To investigate the effects of polysaccharide from Sargassum fusiforme on blood-lipid and antioxidation ability in hyperlipemia rats.Methods The hyperlipemia rats as the experimental animal were established by feeding with fat-rich forage,meanwhile the rats were ig SFPS at doses of 0.4、0.8、1.6g?kg-1for 4wk.The levels of blood-lipid and the indices of antioxidation ability which include the total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),lipid peroxidation(LPO),malonaldehyde(MDA),superoxide dismutase(SOD) and glutathion peroxidase(GSH-Px) were determined.Results and Conclusion SFPS could significantly reduce the contents of TC,TG,LDL-C,LPO and MDA.In the mean time,SFPS increased the content of HDL-C and enhanced the activities of SOD and GSH-Px.
ABSTRACT
Object To study the inhibitory effect of Sargassum fusiforme (Harv.) Setch. polysaccharide (SFPS) on six different kinds of tissue tumor by antitumor experiments in vitro, and to observe tissue speciality of tumor treatment and reveal antitumor effect mechanism. Methods MTT and colony forming methods were adopted to study the antitumor activity of SFPS in vitro. Influences of SFPS on cell cycle and apoptosis were observed. LCM was adopted to measure [Ca 2+ ]i of the cells marked by Fluo 3/AM probe. Results SFPS had content antitumor activities to SGC 7901 and COLO 205. It prevented the transforming of SCG 7901 from G 0/G 1 period to S period. And it increased the apoptosis index (APO%). Applying SFPS, [Ca 2+ ]i increased first and then decreased. Further, after giving CaCl 2, [Ca 2+ ]i increased again. Conclusion By inducing the apoptosis of tumor cells, SFPS shows content antitumor activities to SGC 7901 and COLO 205. SFPS could increase the [Ca 2+ ]i in SGC 7901. And the Ca 2+ are from cellular storage.