Research progress of visual quality after SMILE / 国际眼科杂志(Guoji Yanke Zazhi)

Femtosecond laser small incision lenticule extraction(SMILE)is one of the most advanced corneal refractive operations at present. Different from other traditional corneal refractive operations, SMILE achieves minimally invasive and valveless operation. Therefore, the evaluation of postoperative visual quality of SMILE also has its own characteristics, at present, the latest research at home and abroad has confirmed that the naked eye vision can be significantly improved and the refractive status is stable after SMILE. However, there are different understandings of the objective visual quality indexes and their influencing factors, such as higher-order aberration, modulation transfer function cut off(MTF cut off)and objective scatter index(OSI)after SMILE. This paper reviews the postoperative visual quality and its influencing factors after SMILE to provide clinical help.

Meta-analysis of evaluation on objective visual quality after multifocal intraocular lens implantation / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To review the objective visual quality after multifocal intraocular lens implantation.<p>METHODS:The PubMed, The Cochrane Library, CNKI, Sinomed, Metstr, Wanfang Database were searched for Meta-analysis of the literature on the objective visual quality after multifocal intraocular lens implantation. The retrieval time limit is from the establishment of the database to December 31, 2019. The objective visual quality was evaluated based on the objective visual quality analysis system Ⅱ(OQAS Ⅱ), which included object scatter index(OSI), modulation transfer function cut off(MTF cut off), Strehl ratio(SR), predicted 100% contrast visual acuity(OV100%), predicted 20% contrast visual acuity(OV20%), and predicted 9% contrast visual acuity(OV9%).<p>RESULTS: A total of 8 articles met the inclusion criteria of this study, a total of 432 cases(567 eyes). Meta-analysis showed that there was significant difference between multifocal intraocular lens and monofocal control group in OSI \〖<i>MD</i>=0.66, 95% <i>CI</i>(0.42-0.90), <i>P</i><0.05\〗, but there was no significant difference in MTF cut off \〖<i>MD</i>=-1.94, 95% <i>CI </i>(-5.58-1.70), <i>P</i>=0.30\〗, SR\〖<i>MD</i>=0.00, 95%<i>CI </i>(-0.02-0.02), <i>P</i>=0.87\〗, OV100% \〖<i>MD</i>=-0.02, 95%<i>CI</i>(-0.14-0.11), <i>P</i>=0.76\〗, OV20% \〖<i>MD</i>=-0.03, 95% <i>CI</i>(-0.14-0.08), <i>P</i>=0.60\〗, OV9% \〖<i>MD</i>= -0.01, 95% <i>CI </i>(-0.09-0.07), <i>P</i>=0.82\〗.<p>CONCLUSION: The increase of objective scattering after multifocal intraocular lens implantation may lead to the decrease of visual quality, and the other parameters have no significant difference with the control group. However, no patient follow-up was reported in the included studies. Due to the changes and adaptability after operation, the long-term evaluation of objective visual quality of patients after multifocal intraocular lens implantation needs further research.

Evaluation of use of Volume Conductivity Scatter Parameters as Early Indicator of Sepsis in Elderly Patients: A Hospital Based Case Control Study

Introduction: Sepsis in elderly patients is a critical clinicalissue due to high rates of morbidity and mortality. The modernage cell counters precisely measure volume, size and internalstructure of the cell thereby assessing increase in volumeand cytoplasmic granularity of leukocytes as seen in casesof sepsis. The present study was done to evaluate the useof Volume Conductivity Scatter (VCS) parameters as earlyindicators of sepsis in elderly patients and to overcome theshortcomings of blood culture by using a more precise VCStechnology to assess morphologic changes in cell populationsin sepsis.Material and Method: The study included 51 patients abovethe age of 50 years which were divided into 2 groups Group1, patients with localised infection (n= 39) and Group 2 withsystemic infection (n=12). Group 3 included 30 controlswithout any signs of infection and with total leucocyte countand differential counts within normal limits. Coulter LH 750cellular analysis system was used.Result: Significant increase in both mean neutrophil volume(MNV) and mean monocyte volume (MMV) was reportedamong the patients with sepsis as compared to localizedinfections or control. With a cut off of 145 and 170 for MNVand MMV respectively, a sensitivity of 96% and 91.4% wasobtained in predicting sepsis.Conclusion: Both MNV and MMV have the potential to beused as early indicators of acute bacterial infections.

Evaluation of scatter radiation to the thyroid gland attributable to brain computed tomography scan in Port Harcourt, Nigeria

Background: Computed tomography (CT) is an axial imaging modality that uses X-ray. The study is to determine the amount of X-radiation that reaches the thyroid glands during brain CT.Methods: A prospective study was carried out in Rivers State University Teaching Hospital’s Radiology Department with 60 participants sent for brain CT scan. A 64 slice helical GE Optima CT machine was used while radiation dose reaching the thyroid gland was measured with themoluminiscent dosimeter (TLD) chips (TLD LiF-100). The TLD chip was placed on the anterior aspects of the neck at the level of 6th cervical vertebra (C6) and held in place with adhesive tapes. The TLD chip was later sent to the radiation dosimetric laboratory for reading. Collected data was analyzed using SPSS windows version 22.0 statistical software. A descriptive statistical tool was used to determine central tendencies. Pearson correlation and linear regression analysis models were also used to evaluate correlation between variables.Results: The mean (±SD) CTDI, DLP and brain Effective Dose were 37.265±13.098mGy, 662.451±230.782mGy-cm and 1.667±0.603mSv respectively. The Scatter X-Radiation reaching the thyroid gland and resultant Effective Dose were 5.26±3.13mSv and 0.26±0.16mSv respectively. A positive correlation between brain and thyroid gland effective doses yielded a Pearson’s correlation coefficient (r) of 0.892 within a confidence interval of 0.01 (p value of 0.01) showing significant correlation.Conclusions: The amount of radiation received by the thyroid gland during brain computed tomography scan is significant. Therefore, it is pertinent to protect the thyroid gland during the procedure.

A rare morphological and immunophenotypic presentation of adult B-cell acute lymphoblastic leukemia with blasts in the monocytic zone on CD45 side scatter

We present a case of B-acute lymphoblastic leukemiain an elderly patient who presented with severe weakness and pancytopenia. The patient was a 75 year old Female whose blasts had an unusual morphology in form of coarse azurophilic granules and cytoplasmic blebs and on flow cytometry the blasts were present in the bright CD45 zone with a high side scatter. Bone marrow aspirate sample was subjected to multicolour flow cytometry using Beckman Coulter Navios® which is an 8 colour flow cytometer.Flow cytometricanalysis of the bone marrow aspirate showed blasts in the monocytic zone with a precursor B cell immunophenotype. Complete blood counts showed pancytopenia with peripheral blood film not showingany blasts. Bone marrow aspirate smears showed 20% blasts with coarse azurophilic granules and cytoplasmic blebs.The position of the blasts in this case which were in monocytic zone giving them a bright expression of CD45 and a high side scatter on the CD45 side scatter. This is not the usual positionfor blasts in B-acute lymphoblastic leukemia as these blasts are less complex. A bright expression of CD45 by blasts in B-acute lymphoblastic leukemia is known to be associated with a poor prognosis but the clinical significance of blasts being bright CD45 with a high side scatter is a very rare occurrence and more number of cases with a similar presentation are required to determine a prognostic significance.

Diagnostic value of electrocardiogram scatter plot for arrhythmia / 中国基层医药

Objective To explore the diagnostic value of electrocardiogram (ECG) in common arrhythmias, and to provide an effective reference for clinical diagnosis of arrhythmias.Methods The clinical data of 100 arrhythmia patients in the Department of Cardiology of Rugao Peopleˊs Hospital from July 2016 to June 2018 were analyzed.All the patients were examined by dynamic ECG.The t－RR scatter plot was used to diagnose arrhythmia ,and the diagnosis results were compared between the dynamic ECG and ECG scatter plot.The efficacy of ECG scatter plot in the diagnosis of arrhythmia was analyzed.Results By ECG scatter plot , 22 cases of premature beat ,18 cases of ventricular premature beat,14 cases of atrial fibrillation,15 cases of atrial flutter,8 cases of atrial fibrillation with ventricular early,7 cases of atrial fibrillation with indoor differential transmission ,5 cases of atrial fibrillation combined with atrial flutter,89 cases of correct diagnosis ,and the total coincidence rate was 89.00%.The B line slope of the supraventric－ular premature beat point map was (0.45 ±0.13),which was the largest in each type of arrhythmia ,and the minimum ventricular premature beat was (0.08 ±0.03).The difference in the slope of the supraventricular premature beat and the ventricular premature beat B line was statistically significant (F＝5.031,P＜0.05).The atrial fibrillation,atrial flutter,atrial fibrillation and ventricular early ,atrial fibrillation accompanied with indoor had no statistically significant differences in the slope of B line (all P＞0.05).The sensitivity,specificity and accuracy of ECG in the diagnosis of arrhythmia were 82.05%,90.51%,92.57%, respectively, and the area under the curve was 0.833 (95% CI:0.271－0.942).Conclusion ECG scatter plot has a high application value in the diagnosis of arrhythmias ,and can realize the accurate diagnosis of complex arrhythmias.

Comparison of visual quality following different methods of YAG laser posterior capsulotomy for posterior capsular opacification / 中华实验眼科杂志

Objective To evaluate and compare the subjective and objective visual quality of YAG laser posterior capsulotomy.Methods A series of case observation study was designed.Twenty-eight patients (38 eyes)underwent Nd:YAG laser posterior capsulotomy in the Eye Hospital of Wenzhou Medical University were retrospectively collected from 2015 to 2016.Patients were divided into circular capsulotomy group (20 patients 23 eyes) and cruciate capsulotomy group (12 patients 15 eyes) based on the capsulotomy technique (round incision of posterior capsule or cross incision of posterior capsule).The uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) were examined before and 2 weeks after operation,the intraocular pressure was also measured before operation,1 hour after operation and 2 weeks after operation.The Strehl ratio (SR),modulation transfer function (MTF) cut-off,objective scatter index (OSI),OQAS values (OVs) were analyzed by Two-channel visual quality analysis system OQAS Ⅱ at 1 h and 2 weeks after operation.The changes of intraocular scattering and visual quality at 1 h and 2 weeks after operation with different posterior capsulotomy methods were compared and analyzed.Results At 2 weeks after operation,the UCVA was 0.18±0.15 and 0.16±0.14,and the BCVA was 0.10±0.10 and 0.10±0.11,respectively in the circular capsulotomy group and cruciate capsulotomy group,which was higher than those before operation (all at P＜0.05),but there was no significant difference in UCVA and BCVA between the two groups (all at P＞0.05).There was no significant difference in intraocular pressure between the two groups at 1 hour and 2 weeks after operation (both at P＞0.05).In the circular capsulotomy group,the incidence of black shadow drift was 39.1％ and 30.4％ respectively at 1 hour and 2 weeks after operation,which was higher than that in the cruciate capsulotomy group (6.7％ and 6.7 ％),with no significant differences between the two groups (P =0.065,0.177).No significant difference were found in the incidence of black shadow drift before eyes between the two groups (P＞0.05),and there was no significant differences in the OQAS parameters OSI,MTF,SR,OV100％,OV20％ and OV9％ between the two groups (all at P＞0.05).Conclusions After Nd:YAG laser capsulotomy,the incidence of black shadow drift in the cruciate capsulotomy group is lower than that in the circular capsulotomy group.There is no significant difference in the visual acuity,intraocular pressure,intraocular scattering and visual quality parameters between circular posterior capsulotomy and cross posterior capsulotomy.

Diagnostic value of electrocardiogram scatter plot for arrhythmia / 中国基层医药

Objective@#To explore the diagnostic value of electrocardiogram (ECG) in common arrhythmias, and to provide an effective reference for clinical diagnosis of arrhythmias.@*Methods@#The clinical data of 100 arrhythmia patients in the Department of Cardiology of Rugao People's Hospital from July 2016 to June 2018 were analyzed.All the patients were examined by dynamic ECG.The t-RR scatter plot was used to diagnose arrhythmia, and the diagnosis results were compared between the dynamic ECG and ECG scatter plot.The efficacy of ECG scatter plot in the diagnosis of arrhythmia was analyzed.@*Results@#By ECG scatter plot, 22 cases of premature beat, 18 cases of ventricular premature beat, 14 cases of atrial fibrillation, 15 cases of atrial flutter, 8 cases of atrial fibrillation with ventricular early, 7 cases of atrial fibrillation with indoor differential transmission, 5 cases of atrial fibrillation combined with atrial flutter, 89 cases of correct diagnosis, and the total coincidence rate was 89.00%.The B line slope of the supraventricular premature beat point map was (0.45±0.13), which was the largest in each type of arrhythmia, and the minimum ventricular premature beat was (0.08±0.03). The difference in the slope of the supraventricular premature beat and the ventricular premature beat B line was statistically significant (F=5.031, P<0.05). The atrial fibrillation, atrial flutter, atrial fibrillation and ventricular early, atrial fibrillation accompanied with indoor had no statistically significant differences in the slope of B line (all P>0.05). The sensitivity, specificity and accuracy of ECG in the diagnosis of arrhythmia were 82.05%, 90.51%, 92.57%, respectively, and the area under the curve was 0.833 (95% CI: 0.271-0.942).@*Conclusion@#ECG scatter plot has a high application value in the diagnosis of arrhythmias, and can realize the accurate diagnosis of complex arrhythmias.

Significance of hematology analyzer scatter chart and alarm information in the diagnosis of hematological malignancies / 白血病·淋巴瘤

Objective To study the role of hematology analyzer in the preliminary diagnosis of hematological malignancies. Methods The blood routine results of 121 patients with initial hematological malignancies in the Second Hospital of Shanxi Medical University from October 2016 to February 2017 were analyzed by differential blood count (DIFF) scatter chart and alarm information of hematology analyzer SYSMEX XE-2100. Results Among 121 patients with hematological malignancies, 96 cases (79.3 %) had abnormality of DIFF scatter chart. The DIFF scatter chart of acute lymphoblastic leukemia, chronic lymphocytic leukemia, acute promyelocytic leukemia, and chronic myeloid leukemia had their own distinct characteristics, but the features of other types were not obvious. According to blood smear, blood cells analysis alarmed that the coincidence rates of primitive cells, immature granulocytes, nucleated erythroblasts and atypical lymphocytes were 87.7 % (50/57), 76.3 % (42/55), 33.3 % (19/57), 43.1 % (31/72) respectively. Conclusion The DIFF scatter chart of hematology analyzer and alarm information play very important roles in the diagnosis of hematological malignancies.

The Potential Role of Grid-Like Software in Bedside Chest Radiography in Improving Image Quality and Dose Reduction: An Observer Preference Study

OBJECTIVE: To compare the observer preference of image quality and radiation dose between non-grid, grid-like, and grid images. MATERIALS AND METHODS: Each of the 38 patients underwent bedside chest radiography with and without a grid. A grid-like image was generated from a non-grid image using SimGrid software (Samsung Electronics Co. Ltd.) employing deep-learning-based scatter correction technology. Two readers recorded the preference for 10 anatomic landmarks and the overall appearance on a five-point scale for a pair of non-grid and grid-like images, and a pair of grid-like and grid images, respectively, which were randomly presented. The dose area product (DAP) was also recorded. Wilcoxon's rank sum test was used to assess the significance of preference. RESULTS: Both readers preferred grid-like images to non-grid images significantly (p < 0.001); with a significant difference in terms of the preference for grid images to grid-like images (p = 0.317, 0.034, respectively). In terms of anatomic landmarks, both readers preferred grid-like images to non-grid images (p < 0.05). No significant differences existed between grid-like and grid images except for the preference for grid images in proximal airways by two readers, and in retrocardiac lung and thoracic spine by one reader. The median DAP were 1.48 (range, 1.37–2.17) dGy*cm2 in grid images and 1.22 (range, 1.11–1.78) dGy*cm2 in grid-like images with a significant difference (p < 0.001). CONCLUSION: The SimGrid software significantly improved the image quality of non-grid images to a level comparable to that of grid images with a relatively lower level of radiation exposure.

Distribution characteristics and clinical significance of neutrophil VCS parameters in patients with zinc oxide toxicosis / 临床检验杂志

Objective To observe the clinical value of neutrophil VCS parameters in the patients with zinc oxide toxicosis.Methods The peripheral blood samples from 120 patients with zinc oxide toxicosis,50 patients with bacteria infection and 60 healthy subjects were tested using Coulter LH 750 hematology analyzer.VCS parameters,including mean neutrophil volume (MNV),neutrophil distribution width (NDW),mean neutrophil conductivity (MNC) and mean neutrophil scattering value (MNS),were examined before and after treatment.Results MNV in patients with zinc oxide toxicosis increased significantly compared with those of healthy group and bacterial-infectious group.On the contrary,MNC decreased significantly in the patients with zinc oxide toxicosis compared with that of healthy group and bacterial infection patients.There was significant statistical difference between the two groups (P ＜ 0.01).When the cut-off value is less than 144.70,the sensitivity and specificity of differential diagnosis are 90.83％,98.33％,respectively,higher than those of WBC count and other VCS parameters.The values of MNC in WBC greater than or equal to 10.0 × 109/L group and WBC less than 10.0 × 109/L group were (140.05 ±4.26) and (138.87 ±5.87) respectively.There was no significant difference between the two groups (P ＞ 0.05).The values returned to normal range after treatment for 3 days.Conclusion The MNC of neutrophil may reflect the morphologic change of neutrophils in zinc oxide toxicosis and restore its normal value after treatment,so it can be used as a marker for diagnosis and monitoring of disease.

Resistant phenotype and correlation analysis of ESBL-producing enterobacteriaceae between Cefotetan and Ceftazidime / 国际检验医学杂志

Objective To explore the clinical application of antibiotics Ceftazidime(CAZ) and Cefotetan(CTT)by analysis susceptibility and scatter of the CAZ adn CTT against Escherichia coli(ECO) and Klebsiella pneumoniae(KPN).Methods The drug sensitivity analysis of 1 311 strains of ECO and 898 strains of KPN isolated from 2012 to 2015 and the relationship between CAZ and CTT was analyzed by using the Whonet 5.6 software.Results The resistance rate of ESBL+ KPN to CAZ was 41.2％ and the rate to CTT was 14.1％,the difference was statistically significant(P<0.05).The resistance rate of ESBL+ ECO to CAZ was 34.6％ and the rate to CTT was 1.1％,the difference was statistically significant(P<0.05).The average value of MIC of CAZ was highest in group of ESBL+KPN,it was 6.39 μg/mL.And it was lowest in group of ESBL-KPN,it was 1.37 μg/mL.The average value of MIC of CTT was highest in group of ESBL+KPN,it was 6.8 μg/mL.And the lowest was in group of ESBL-KPN.The range of MIC of CAZ was 1-64 μg/mL,and the range of CTT was 4-64 μg/mL in all groups.The cross sensitivity of CAZ and CTT was more than 90.0％.The cross resistance was less than 5.0％.The cross sensitivity of CAZ and CTT was less than 70.0％ in ESBL+ group.And the cross resistance was up to 13.4％.Conclusion The cross resistance and cross sensitivity of the two antibiotics is very important in guiding clinical antibiotic selection or replacement.

Side scatter versus CD45 flow cytometric plot can distinguish acute leukaemia subtypes.

Background & objectives: Flow cytometry is an important tool to diagnose acute leukaemia. Attempts are being made to find the minimal number of antibodies for correctly diagnosing acute leukaemia subtypes. The present study was designed to evaluate the analysis of side scatter (SSC) versus CD45 flow dot plot to distinguish acute myeloid leukaemia (AML) from acute lymphoblastic leukaemia (ALL), with minimal immunological markers. Methods: One hundred consecutive cases of acute leukaemia were evaluated for blast cluster on SSC versus CD45 plots. The parameters studied included visual shape, CD45 and side scatter expression, continuity with residual granulocytes/lymphocytes/monocytes and ratio of maximum width to maximum height (w/h). The final diagnosis of ALL and AML and their subtypes was made by morphology, cytochemistry and immunophenotyping. Two sample Wilcoxon rank-sum (Mann Whitney) test and Kruskal-Wallis equality-of-populations rank tests were applied to elucidate the significance of the above ratios of blast cluster for diagnosis of ALL, AML and their subtypes. Receiver operating characteristic (ROC) curves were generated and the optimal cut-offs of the w/h ratio to distinguish between ALL and AML determined. Results: Of the 100 cases, 57 of ALL and 43 cases of AML were diagnosed. The median w/h ratio of blast population was 3.8 for ALL and 1 for AML (P<0.001). ROC had area under curve of 0.9772.The optimal cut-off of the w/h ratio for distinction of ALL from AML was found to be 1.6. Interpretation & conclusions: Our findings suggest that if w/h ratio on SSC versus CD45 plot is less than 1.6, AML may be considered, and if it is more than 1.6, ALL may be diagnosed. Using morphometric analysis of the blast cluster on SSC versus CD45, it was possible to distinguish between ALL and AML, and their subtypes.

Volume, conductivity, and scatter parameters as diagnostic aid to bacterial sepsis: A tertiary care experience.

Introduction and Materials and Methods: Early diagnosis of sepsis is extremely important to reduce high mortality and morbidity. In this study, clinical usefulness of the volume, conductivity and scatter parameters (mean channels of cell volume, conductivity, and light scatter) in neutrophils was analyzed for predicting acute bacterial infection, which are obtained by the Coulter LH 750 Hematology Analyzer (Beckman Coulter, Fullerton, CA, USA) during automated differential counts. Results: Peripheral blood samples from 162 patients with positive blood cultures for bacteria and 40 healthy controls were studied. We observed a significant increase in the mean channel of neutrophil volume (MNV) from septic patients compared with control subjects (156 ± 13.5 vs. 143 ± 4.8; P < 001). Discussion and Conclusion: An elevation of the MNV was associated with a higher white blood cell count and percentage of neutrophils and was present even in patients who did not have leukocytosis or neutrophilia. With a cut-off of 149 for the MNV, a specificity of 91.4% and sensitivity of 88.7% were achieved. As a quantitative, objective, and more sensitive parameter, we propose that the MNV has a potential to be an additional indicator for acute bacterial infection.

Nest site selection by individual leatherback turtles (Dermochelys coriacea, Testudines: Dermochelyidae) in Tortuguero, Caribbean coast of Costa Rica / Selección del sitio de anidación de individuos de tortuga baula (Dermochelys coriacea, Testudines: Dermochelyidae) en Tortuguero, costa Caribe de Costa Rica

Nest site selection for individual leatherback sea turtles, Dermochelys coriacea, is a matter of dispute. Some authors suggest that a female will tend to randomly scatter her nests to optimize clutch survival at a highly dynamic beach, while others suggest that some site fidelity exists. It is also possible that both strategies exist, depending on the characteristics of each nesting beach, with stable beaches leading to repeating nest site selections and unstable beaches leading to nest scattering. To determine the strategy of the Tortuguero population of D. coriacea, female site preference and repetition were determined by studying whether females repeat their nest zone choices between successive attempts and whether this leads to a correlation in hatching and emergence success of subsequent nests. Nesting data from 1997 to 2008 were used. Perpendicular to the coastline, open sand was preferred in general, regardless of initial choice. This shows a tendency to scatter nests and is consistent with the fact that all vertical zones had a high variability in hatching and emergence success. It is also consistent with nest success not being easily predictable, as shown by the lack of correlation in success of subsequent nesting attempts. Along the coastline, turtles showed a preference for the middle part of the studied section of beach, both at a population level and as a tendency to repeat their initial choice. Interestingly, this zone has the most artificial lights, which leads to slightly lower nest success (though not significantly so) and hatchling disorientation. This finding merits further study for a possibly maladaptive trait and shows the need for increased control of artificial nesting on this beach.

La selección de sitio de anidación por parte de individuos de la tortuga baula, Dermochelys coriacea, es tema de discusión. Algunos autores sugieren que una hembra dispersa sus nidos al azar para maximizar su éxito en las inestables playas que elige para anidar, mientras que otros sugieren que existe cierta fidelidad al sitio de anidación. También es posible que ambas estrategias existan, dependiendo de las características de la playa donde anidan, así playas estables llevarían a las hembras a repetir la selección de sitio mientras que las playas inestables las llevarían a dispersar sus nidos. Para determinar la estrategia que usan las hembras de D. coriacea de Tortuguero, se estudió la preferencia de sitio de las hembras así como su repetición en este sitio, desde 1997 hasta 2008. Para la selección de sitio vertical (perpendicular a la costa), las hembras en general prefirieron anidar en arena abierta, independientemente de su escogencia inicial. Esto muestra una tendencia a dispersar los nidos y es consistente con la alta variación en los éxitos de eclosión en todas las zonas, así como la falta de relación entre los éxitos de eclosión de nidos subsecuentes, lo cual muestra que el éxito de los nidos no es predecible. Para la selección horizontal (a lo largo de la costa) se prefirió la parte media de la sección estudiada de la playa, tanto a nivel poblacional como individual (repetición de selección de sitio). Interesantemente, esta zona tiene la mayor cantidad de luces artificiales lo cual lleva a éxitos de eclosión ligeramente más bajos (aunque no significativamente) y a la desorientación de neonatos. Este resultado amerita estudios futuros para determinar si se trata de una tendencia maladaptativa en la población y muestra la importancia de controlar la cantidad de luz artificial en esta playa como medida de conservación.

Study on the changes of peripheral blood lymphocyte scatter diagram in patients newly diagnosed with hepatitis B / 国际检验医学杂志

Objective To study the clinical significance of leukocyte parameters measured by using automatic blood cell analyzer SysmexXE‐2100inpatientsnewlydiagnosedwithhepatitisB.Methods 96patientsnewlydiagnosedwithhepatitisB(observation group) and 100 people who underwent healthy examination during the same period(control group) were recruited in the study .Rel‐ative indicators were measured for the people mentioned above ,such as the DNA of Hepatitis B virus(HBV‐DNA) ,Hepatitis B sur‐face antigen(HBsAg) ,aspartate aminotransferase(AST ) ,alanine aminotransferase(ALT ) ,lymphocytes parameters Lymph‐Y and Lymph‐X ,neutrophils parameters Neut‐X and Neut‐Y .The test results were recorded and statistically analyzed by using software SPSS19 .0 .Results The differences of Lymph‐X ,Neut‐Y and Neut‐X were not statistically significant compared between observa‐tion group and control group(P>0 .05) ,while the differences of HBV‐DNA ,HBsAg ,AST ,ALT and Lymph‐Y were statistically significant compared between the two groups(P<0 .05) .In addition to that ,the parameter Lymph‐Y was positirely correlated with HBV‐DNA(r=0 .160 ,P=0 .026) and HBsAg(r=0 .149 ,P=0 .037) .Conclusion The peripheral blood lymphocytes parameter Lymph‐Y of patients newly diagnosed with hepatitis B is higher than healthy people ,which makes it possible to become an indicator for differential diagnosis .

The comparison of reticulocyte parameters on the XN-1000 and LH780 hematology analyzer / 国际检验医学杂志

Objective To analyze the comparability and correlation of reticulocyte parameters on the Beckman‐Coulter LH780 (LH780)and Sysmex XN‐1000(XN‐1000)hematology analyzers .Methods 80 blood samples were measured by the two instru‐ments ,the RBC count ,percentage of Ret(Ret% ) ,Ret value(Ret#)and immature reticulocyte fraction(IRF) were analyzed ,and the correlation of Ret% ,Ret# and IRF between two instruments were also analysed .The correlation between percentage of high laser reticulocyte(HLR% ) of LH780 and percentage of middle fluorescent reticulogyte(MFR% )+percentage of high fluorescent reticu‐locyte(HFR% ) of XN‐1000 were compared using two calculation methods of each instrument ,and the application value of HLR%were analysed .Results No significant differences were founded in RBC count ,Ret% ,and Ret# between the two instruments(P>0 .05) ,while there was statistical difference in IRF between the the two instruments (P<0 .05) .The relative deviation coincidence rate of RBC count was 97 .5% ,the correlation coefficent (r) of Ret% ,Ret# and IRF were 0 .912 ,0 .895 and 0 .666 respectively . There were statistical differences and correlations between HLR% and MFR% + HFR% when using calculation method of XN‐1000 and LH780 respectively(r were 0 .666 and 0 .767 respectively ,P<0 .05) .Conclusion The RBC count could meet the matc‐hing requirement ,and the Ret% and Ret # may be highly correlated on the two instruments .While the reference range of IRF should be established in each instrument .

How Effective Are Radiation Reducing Gloves in C-arm Fluoroscopy-guided Pain Interventions?

BACKGROUND: The physician's hands are close to the X-ray field in C-arm fluoroscopy-guided pain interventions. We prospectively investigated the radiation attenuation of Proguard RR-2 gloves. METHODS: In 100 cases, the effective doses (EDs) of two dosimeters without a radiation-reducing glove were collected. EDs from the two dosimeters-one dosimeter wrapped with a glove and the other dosimeter without a glove- were also measured at the side of the table (Group 1, 140 cases) and at a location 20 cm away from the side of the table (Group 2, 120 cases). Mean differences such as age, height, weight, radiation absorbed dose (RAD), exposure time, ED, and ratio of EDs were analyzed. RESULTS: In the EDs of two dosimeters without gloves, there were no significant differences (39.0 +/- 36.3 microSv vs. 38.8 +/- 36.4 microSv) (P = 0.578). The RAD (192.0 +/- 182.0 radcm2) in Group 2 was higher than that (132.3 +/- 103.5 radcm2) in Group 1 (P = 0.002). The ED (33.3 +/- 30.9 microSv) of the dosimeter without a glove in Group 1 was higher than that (12.3 +/- 8.8 microSv) in Group 2 (P < 0.001). The ED (24.4 +/- 22.4 microSv) of the dosimeter wrapped with a glove in Group 1 was higher than that (9.2 +/- 6.8 microSv) in Group 2 (P < 0.001). No significant differences were noted in the ratio of EDs (73.5 +/- 6.7% vs. 74.2 +/- 9.3%, P = 0.469) between Group 1 and Group 2. CONCLUSIONS: Proguard RR-2 gloves have a radiation attenuation effect of 25.8-26.5%. The radiation attenuation is not significantly different by intensity of scatter radiation or the different RADs of C-arm fluoroscopy.

The Effects of Overnight Orthokeratology Lens Wear on Ocular Scatter

PURPOSE: To evaluate the change of visual quality after wearing orthokeratology lenses overnight. METHODS: This study included 13 patients (24 eyes) who wore orthokeratology lenses for the first time. Visual quality was assessed with the optical quality analysis system (OQAS) using the double-pass technique. The value of modulation transfer function (MTF) cutoff, Strehl ratio, and objective scatter index (OSI) were measured at baseline and 4 weeks after wearing the lenses. RESULTS: The mean spherical equivalent decreased from -2.70 +/- 1.03 diopters (D) to 0.03 +/- 0.36 D after 4 weeks of wearing orthokeratology lenses and uncorrected visual acuity improved from log MAR 0.88 +/- 0.17 to log MAR 0.03 +/- 0.04. The MTF cutoff decreased from 38.20 +/- 11.01 to 34.79 +/- 10.30 and the Strehl ratio decreased from 0.24 +/- 0.07 to 0.21 +/- 0.07 but without statistical significance. The OSI significantly increased from 0.38 +/- 0.19 to 0.72 +/- 0.40 after wearing orthokeratology lenses. CONCLUSIONS: Orthokeratology lenses were effective for correction of myopia and astigmatism. The OSI increased after wearing the lenses, but MFT cutoff and Strehl ratios were not affected.

Simultaneous occurrence of chronic myeloid leukemia and chronic lymphocytic leukemia: Report of an unusual case.

The coexistence of chronic myeloid leukaemia(CML) and chronic lymphocytic leukemia(CLL) has been reported occasionally in literature, with only seven cases of simultaneous occurrence of these two diseases. We present here a case of 57 yr male patient where a complete blood count and differential done using volume conductivity scatter (VCS) technology suggested a diagnosis of CML with CLL. It was further confi rmed by immunophneotyping and cytogenetic analysis. The patient was started on tyrosine kinase inhibitor, 400 mg once daily. Four months after the treatment, patient is doing fi ne with a count of 22 × 109/L and 64% lymphocytes.