ABSTRACT
Aim To study the effect of total saponins of Schizocapsa plantaginea Hance ( SFSP) on hepatic fibrosis in rats induced by CC14 and to investigate the molecular mechanisms of these effects. Methods 5 SD rats were randomly divided into five groups: Normal control group, model group, colchicine (Col) as positive control group, low-dose SFSP group (SFSP-L), and high-dose SFSP group ( SFSP-H ). After four weeks of continuous administration, serum AST, ALT and hydroxyproline (Hyp) were measured by biochemical detection method. The four indicators of liver fibrosis ( CIV, PC HJ , LN, HA) in serum were measured by enzyme linked immunosorbent assay. The location of liver fibrous tissues was observed by Masson staining. Liver sections were stained with HE, and observed for pathologic changes. Liver ultrastructural changes were observed using a transmission electron microscope. The expressions of mRNA of α-smooth muscle actin(α-SMA) , transforming growth factor pi (TGF-β1) , Smad4 and Smad7 in liver were detected by qPCR. Results In the liver fibrosis model group, the contents of AST, ALT and CIV, PCI, LN, HA, Hyp reflecting liver injury and fibrosis significantly increased ( P < 0. 05 ) , while in SFSP-H group and Col group the contents of the above indicators were significantly reduced, and the pathological results of liver tissues were consistent with it. SFSP significantly inhibited the expression of TGF-β1, Smad4 and α-SMA mR-NA ( P < 0. 01 ) , and increased the expression of Smad7 as well (P < 0. 01). Conclusion SFSP has an antifibrogenic effect through down-regulating the TGF-(β1/Smad signaling pathway in rats.
ABSTRACT
Object\ To provide a basis for the further development, proper utilization and formulation of a standard for the medicinal materials Methods\ By macro and microscopic studies in combination with physicochemical identification The possible therapeutic value of other parts of the plant was briefly discussed Results\ Four obvious distinctive features were observed: ① Stem tuber showed a curved kidney like shaped ② Parenchymatous cells were polygonal shaped and densely packed together with limited intercellular spaces ③ Rich in compound starch grains, and ④ Crystalline calcium oxalate Clusters can be seen. The tubers had an absorbance peak in UV specturm at ? 241 nm , but its adventitious root was devoid of such absorbance peak Conclusion\ The distinctive features are clear and can be mastered easily The adventitious roots must be discarded when the tubers were used as medicine