ABSTRACT
OBJECTIVE: To synthesize cis-stilbenoids derivatives and investigate their anti-inflammatory activities in vitro. METHODS: Sciryagarol I(1) from the tuber of Scirpus yagara, was used as a primary material. The synthetic derivatives were obtained via methyltion, acetylation, and demethylation. MTT assay was used to detect the effects of compound 1, its synthetic derivatives 3-5 and sciryagarol II(2) on RAW264.7 cells viability. And the inhibitory activities of compounds 1-5 against TNF-α and IL-6 production in LPS- and Pam3csk4-activated RAW264.7 cells were evaluated by ELISA assay. RESULTS: The compounds 3-5 were synthesized, and their structures were characterized by 1H-NMR and HRESI-MS spectra. ELISA assay showed that compounds 1, 2 and 5 significantly inhibited the production of TNF-α and IL-6 from LPS- and Pam3csk4-stimulated RAW264.7 cells. CONCLUSION: Three novel compounds 3-5 are synthesized. The number of phenolic hydroxyl in the structure of cis-stilbenoids may be related to their anti-inflammatory activities.
ABSTRACT
OBJECTIVE: To study the chemical constituents of Scirpus yagara Ohwi. METHODS: Column chromatography techniques were applied to isolate the constituents. The chemical structures of the constituents were elucidated on the basis of physicochemical properties and spectral data. RESULTS: Forteen compounds were isolated and identified as sanleng diphenyllactone (1), β-sitosterol (2), ferulic acid(3), succinic acid(4), azelaic acid(5), docosanoic acid (6), 6, 7, 10-trihydroxy-8-octadecenoic acid(7), 4, 4-dime-thyl-1, 7-pimelic acid(8), vanillic acid(9), 3, 5-dihydroxy-4-methoxy-benzoic acid(10), p-hydroxybenzaldehyde(II), 3, 4-dihydroxy-benzoic acid(12), 2, 7-dihydroxy xanthone(13) and daucosterol(14). CONCLUSION: Compounds 1-10 and 12-14 are obtained from this plant for the first time, and compounds 1 and 13 were obtained from the genus Scirpus for the first time.