ABSTRACT
The alkaloid securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.). Spore germinations of all the tested fungi were inhibited. Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).
Subject(s)
Alternaria , Azepines , Brassica , Colletotrichum , Fungi , Germination , Helminthosporium , Heterocyclic Compounds, Bridged-Ring , Lactones , Musa , Phyllanthus , Piperidines , Plants , SporesABSTRACT
Crude extracts and active principles from medicinal plants have shown potential role in controlling plant diseases in glasshouses as well as in fields as one of the safest and ecofriendly methods. The effect of nor-securinine (an alkaloid) isolated from Phyllanthus amarus has been seen against spore germination of some fungi (Alternaria brassicae, A. solani, Curvularia pennisetti, Curvularia sp., Erysiphe pisi, Helminthosporium frumentacei) as well as pea powdery mildew (Erysiphe pisi) under glasshouse conditions. The sensitivity of fungi to nor-securinine varied considerably. Nor-securinine was effective against most of the fungi. H. frumentacei was more sensitive even at the lowest concentration (1,000 microg/ml). Likewise conidia of E. pisi were also inhibited in partially or completely appressorium formation. Pre-inoculation treatment showed greater efficacy than post-inoculation in inhibiting powdery mildew development on pea plants in a glasshouse. Maximum inhibition occurred at 2000 microg/ml
Subject(s)
Brassica , Complex Mixtures , Fungi , Germination , Helminthosporium , Pisum sativum , Phyllanthus , Plant Diseases , Plants, Medicinal , Spores , Spores, FungalABSTRACT
ObjectiveTo explore more effective and lower expensive approach for aplastic anemia(AA).Methods A prospective,randomized clinical trial was conducted to determine whether the outcome of AA patients treated with the combination of securinine plus anisodamini dripping in bone marrow(therapy group)was better than that with anisodamini alone dripping in vein(control group).Results The cure remission rate(83.3%) and the response rate(94.4%) of therapy group were significantly higher than that of control group(11%,55.6%, P
ABSTRACT
Aim To investigate the effect of the GABAA receptor on the analgesia,hypnosis and amnesia induced by GABA in mice.Methods GABA was intracerebroventricularly(icv) injected and securinine,a GABAA receptor antagonist,was intravenously(iv) injected after the GABA intracerebroventricularly injection.Then four techniques including hot-plate test,acetic acid-induced writhing test,awaken test as well as step-through test & step-down test were employed to evaluate the effects on the pain index in hot-plate test(HPPI),the writhing times,the sleeping time(ST) and the latency & the number of errors.Results GABA increased the HPPI and ST,but decreased writhing times and the latency & increased the number of errors in conscious mice;the above effects are dose-dependent.Securinine could antagonize the effects stimulated by GABA.Conclusion GABA lead to analgesia,hypnosis and amnesia.GABAA receptor might be the important target for these effects.
ABSTRACT
0.05). CONCLUSION GABA A receptor may not be the major target of enflurane, isoflurane and sevoflurane.