ABSTRACT
Objective To establish a QTRAP-UFLC-MS/MS method for the determination of 15 kinds of amino acids and 10 kinds of nucleosides in the seeds of Annona squamosa from different producing areas, toanalyze and investigate the difference of the 25 components in samples from five habitats, and provide scientific basis for its comprehensive utilization. Methods The analysis was carried out on a Waters XBridge Amide column (100 mm × 2.1 mm, 3.5 μm) with elution by mobile phase of 0.2% formic acid in water (A)-0.2% formic acid in acetonitrile (B) at a flow rate of 0.6 mL/min. The column temperature was maintained at 30 ℃. The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of the seeds from A. squamosa in five habitats was carried out by PCA and TOPSIS analysis. Results All 15 kinds of amino acids and 10 kinds of nucleosides showed good linearity (r2 > 0.994 7). The RSDs of the precision, repeatability, and stability tests were less than 3.87%. The average recovery rates were in the range from 94.90% to 98.78%, RSDs were in the range from 0.84% to 3.01%. There are differences in the composition and content of amino acids and nucleosides in the seeds of A. squamosa from different habitats, among which Hainan produced the highest comprehensive quality. Conclusion The method is simple, accurate, and reliable, which can provide the reliable basis for quality evaluation of the seeds from A. squamosa, and samples from different habitats can be optimized according to the target component.
ABSTRACT
Objective To study the chemical composition from the seeds of Annona squamosa. Methods The components of annonaceous acetogenins from the seeds of A. squamosa were isolated and purified by extracting and chromatography separation. The structures of the compounds were identified by their physicochemical properties, UV, NMR, and mass spectrometry data. Results Seven annonaceous acetogenins were isolated from the ethyl acetate extract of the seeds from A.squamosa. They are 3-[7-[5- [1,4-dihydroxy-4-[5-(1-hydroxytridecyl)-2-furanyl] butyl] tetrahydro-2-furanyl]-2-hydroxyheptyl]-5-methyl-2(5H)-furanone (1), 3-[9-[5-(1-hydroxy-4-heptadeceneyl) tetrahydro-2-furanyl] hydroxynonyl]-5-methyl-2 (5H)-furanone (2), bullatalicin (3), squamostatin A (4), squamostatin D (5), bullatacin (6), and 10-hydroxyasimicin (7). Conclusion Compounds 1 and 2 are two new compounds, named as trilobalicin I (1) and D20-solamin B (2), respectively.