ABSTRACT
Objective To investigate the effects of two biovars of ureaplasma urealyticum on leukocyte concentration and sperm motion parameters.Methods Sperm motion parameters were analyzed with computer-aided sperm analysis (CASA).Leukocytic number of semen was measured with benziding-perxidase staining.Culturing and real-time polymerase chain reaction (RT-PCR) was used to identify biovars of U.urealyticum in the semen.Results Sperm motion parameters including mean curvilinear velocity (VCL), mean straight-line velocity (VSL), mean average path (VAP) , and mean beat Cross frequency (BCF) showed significant difference between infertile and normal group (P ＜0.05).Biovar Ⅱ infected men displayed significant increase in leukocyte counts (P ＜ 0.001).When compared to uninfected group,the sperm motion parameters showed significant decreases of VCL, VSL, VAP, mean percentage lineality (LIN), and swing(WOB) in biovar Ⅱ (P ＜ 0.05).Conclusions Biovar Ⅱ has closer relationship with leukocyte elevation and sperm motion impairment.