Clinical efficacy of selenium-enriched garlic on patients with oligoasthenospermia / 中国医师杂志

Objective To investigate the clinical efficacy of selenium-enriched garlic on patients with oligoasthenospermia.Methods From October 2012 to August 2013,160 patients with oligoasthenospermia were randomly divided into two groups.Treatment measurements for 80 patients in the control group included oral treatment of Shengjing capsules 1.6 g/time Tid,Vit-E soft capsules 100 mg/time Tid,and took Vit-B 0.2 g/time Tid,for three months.Treatment measurements for 80 patients in the treatment group included oral treatment of selenium-enriched garlic in addition to all the above,for three months.We observed the changes of the semen quality indexes (including semen volume,sperm density,motility,survival rate,etc.) of two groups before and after treatment.Results Sperm quality was improved after treatment in both groups.All indices in the treatment group were significantly higher than those in the control group (P ＜ 0.05).Conclusions For patients with male infertility caused by oligoasthenospermia,The use of combination of selenium-enriched garlic and Shengjing capsules can improve the quality of sperm.

Preeclampsia en pacientes infértiles llevadas a inseminación homóloga y heteróloga en el Centro de Biomedicina Reproductiva del valle - Fecundar, Cali, Colombia / Preeclampsia in infertile patients subjected to homologous and heterologous insemination in the centro de Biomedicina Reproductiva del Valle - Fecundar, Cali, Colombia

Objetivo: determinar la incidencia y el riesgo de preeclampsia en pacientes infértiles a las que se les realizó inseminación con semen de donante o de su pareja. Materiales y métodos: cohortes históricas de pacientes a las cuales se les realizó inseminación intrauterina (IIU) homóloga o heteróloga y que lograron embarazo mayor de 20 semanas de gestación, entre enero del 2000 al 31 de diciembre del 2009 en el Centro de Biomedicina Reproductiva del Valle - Fecundar. Se excluyeron pacientes con trastornos endocrinos y metabólicos (Diabetes Mellitus, enfermedades del colágeno) o enfermedades crónicas (hipertensión arterial, trombofilias). El tamaño de la muestra fue de 155 pacientes expuestas a inseminación heteróloga y 310 a inseminación homóloga. Se evaluó la presencia preeclampsia y el resultado perinatal, así como el tipo de inseminación, edad, raza, nivel educativo, índice de masa corporal (IMC en kg/m²), indicación de la inseminación, número de ciclos previos y estrato socioeconómico. Se estimó la incidencia acumulada y se compararon las dos cohortes por medio del RR con su respectivo intervalo de confianza. Se estratificó por edad. Resultados: 428 mujeres cumplieron los criterios de selección. La incidencia de preeclampsia se presentó en un 6% (24/428), un 4% (11/264) en las IIU homólogas y 7,93% (13/164) de las IIU heterólogas. Se encontró que no hay diferencias en el riesgo de preeclampsia de acuerdo con el tipo de inseminación y al ajustar por edad RR: 1.18 (IC 95%: 0,54-2,58). Conclusiones: en la IIU heteróloga se encontró una incidencia mayor de preeclampsia. Sin embargo, no hay asociación con el desarrollo de preeclampsia.

Objective: Determining preeclampsia incidence and risk in infertile patients who have been inseminated with semen from a donor or from their partner. Materials and methods: This study dealt with historical cohorts of patients who had been subjected to homologous or heterologous intrauterine insemination in the Fecundar infertility centre and who had managed to become pregnant for more than 20 weeks gestation between January 2000 and 31st December 2009. Patients suffering endocrine and metabolic disorders (diabetes mellitus, collagen diseases) or chronic diseases (arterial hypertension, thrombophilia) were excluded. Sample size was 155 patients regarding heterologous insemination and 310 for homologous insemination.The presence of preeclampsia and perinatal result were evaluated and type of insemination, age, ethnicity, educational level, body mass index (BMI in Kg/m²), indication of insemination, number of prior cycles and socioeconomic strata were also measured. Accumulated incidence was estimated and two cohorts were compared by RR with respective confidence intervals. Females were stratified by age. Results: 428 females fulfilled the selection criteria. There was 6% (24/428) preeclampsia incidence, 4% (11/264) homologous IUI and 7.93% (13/164) heterologous IUI. No differences were found regarding the risk of preeclampsia according to the type of insemination and after being adjusted for age (1.18 RR; 0.54-2.58 95%CI). Conclusions: Greater preeclampsia incidence was found in heterologous IUI; however, there was no association with the development of preeclampsia.

Análise espermática de perdizes (Rhynchotus rufescens) criadas em cativeiro e suplementadas com selênio / Semen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with selenium

Considerando a importância comercial da perdiz (Rhynchotus rufescens), implantaram-se algumas biotecnologias reprodutivas. Trinta animais, provenientes da FCAV-UNESP/Jaboticabal (2007-2008), foram aleatoriamente distribuídos em grupos: controle (sem selênio orgânico) e tratamento (com 0,2 a 0,8 mg de selênio em 1000 kg de ração ). Coletou-se sêmen por excitação manual, que foi aliquotado em pools com 150 μL. Mensuraram-se volume, motilidade, vigor, número de espermatozoides, concentração e morfologia espermáticas. Diluíram-se 20 μl de sêmen em 300 μl de solução fisiológica para testes complementares (Integridade das membranas acrossomal e plasmática). Contaram-se 200 células, por teste, e classificaram-nas: 1) Acrossomo Íntegro: cor lilás e Não-Íntegro: róseo; 2) Células Vivas (não coradas) e Mortas (coradas). Os dados foram analisados pelo SAS, System for Windows. Os resultados dos pools com e sem selênio foram: as variáveis volume, motilidade, vigor, número de espermatozoides, concentração, integridade acrossomal e integridade da membrana plasmática não apresentaram resultados significantes, porém encontrou-se uma menor porcentagem de espermatozoides com peça intermediária fortemente dobrada, nos animais tratados com selênio em relação aos não tratados (1,33 ± 0,53 vs. 3,78 ± 0,69, respectivamente; p = 0.0107). De fato, sabe-se que o selênio tem papel importante na estrutura dos espermatozoides20. A deficiência de selênio está associada com danos na arquitetura da peça intermediária do espermatozóide.

Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV – UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 μL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 μL was diluted in 300 μL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.