ABSTRACT
ABSTRACT Celosia argentea L., Amaranthaceae, is widely used as traditional medicine with a long history in China. It is a unique source of Semen Celosiae whose contributions include purging the hepatic pathogenic fire, improving eyesight, and treating other eye diseases. Over 79 compounds from this plant were isolated and identified, mainly including saponins, peptides, phenols, fatty acids, and amino acids, of which saponins have been considered as the characteristic and active constituents of Celosia argentea. Experimental evidences manifested that Celosia argentea, with its active compounds, possesses wide-reaching biological activities such as hepatoprotection, tumor treatment, anti-diarrhea, anti-diabetes, anti-oxidant, anti-hypertension, and for treatment of a number of eye diseases. The objective of the study was to provide an overview of the ethno-pharmacology, chemical constituents, pharmacology, and related clinical applications of Celosia argentea, and to reveal their therapeutic potentials, and secure an evidence base for further research works on Celosia argentea.
ABSTRACT
Objective: To determine the contents of celosin A and celosin B in Semen celosiae. Methods: HPLC was carried out with an Agilent Alliance, Model 1100, equipped with Elite ODS C18 column (4.6mm X 250mm, 5 μm) and evaporated light scattering detector (ELSD). The mobile phase (acetonitrile-0.1% glacial acetic acid) was eluted in gradient mode. Results: Celosin A and the peak area showed a good linearship within the range of 2. 5-30.0 μg, with the recovery rate being 99.89% (n=6) and RSD being 1.85%. Celonsin B and the peak area showed a good linearship within the range of 1.25-15.0 μg, with the recovery rate being 98.98% (n=6) and the RSD being 1.78%. Contents of celosin A and celosin B in 8 Semen celosiae samples on medical markets were obviously different from each other, ranging from (0.051 2±0.001 6)%, to (0.114 3 ± 0.000 8)% and (0.010 9±0.001 9)% to (0.081 5±0.000 9)%. Conclusion: We have established an HPLC-ELSD method for determining the effective contents of Semen celosiae; the method is accurate and simple.