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Five compounds were isolated from the ethyl acetate fraction of Semen Persicae by using various chromatographic methods, including ODS, Sephadex LH-20, HPLC and semipreparative HPLC. Their structures were identified by 1D-NMR, 2D-NMR, HR-ESI-MS, UV, IR, circular dichroism (CD) and ECD calculation techniques: (2R,3R)-5,7,4′-trihydroxy-3′-methoxy-3-formylflavan-3-ol-5-O-β-D-glucopyranoside (1), (7R,8S)-dihydrodehydrodiconiferyl 6″-benzoyl alcohol-9-O-β-D-glucopyranoside (2), (7R,8S)-dihydrodehydrodiconiferyl alcohol-9-β-O-D-glucopyranosid (3), 2-methoxy-4-(2-propenyl)-phenyl-O-β-D-glucopyranoside (4), 2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-propane-1,3-diol (5). Compound 1 and 2 are new compounds, and compounds 3-5 were obtained from Prunus davidiana (Carr.) Franch. for the first time.
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Objective To study the effect of compatibility of Flos Carthami and Semen Persicae in different ratios on the contents of hydroxysafflor yellow A(HSYA) in their water decoction.Methods Reverse-phase HPLC was performed on a Luna C18 column(4.6 mm?150 mm,5?m) at 30℃.The mobile phase consisted of methanol-0.12 mmol?L-1 phosphate solution(28∶72,V/V) at the flow rate of 1.0 mL? min-1.The detector was operated at wavelength of 403nm.Results A good linearity of HSYA was obtained in the range of 4.0～160.0?g?mL-1(Y=25 396X+40 247,r=0.999 7),the average recovery was 94.76% and the RSD(n=6) was 2.93%.The amount of HSYA increased with the ratio of Flos Carthami,but had no correlation with the ratio of Semen Persicae.Conclusion The HPLC method for determination of HSYA is simple,fast and accurate.It can be used for the quality control of Flos Carthami and its compound preparations.Semen Persicae has no effect on the extraction of HSYA.
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Objective To explore the effects of alcoholic extracts of traditional Chinese medicines on the post-translational processing and trafficking of tyrosinase.Methods Human YUGEN8 amelanotic melanoma cells were grown in vitro;the cells were incubated with one of the seven traditional Chinese medicines,including Rhizoma Chuanxiong and psoralen.Protein analysis with Western blot,enzymolysis with endoglycosidase H (Endo H),and subcellular localization with laser confocal microscopy were per- formed.The expression,maturity and export from endoplasmic reticulum (ER) of tyrosinase in the treated cells were compared with those in the untreated controls.Results Compared with controls,an approximate- ly 80-kDa,Endo H-resistant tyrosinase doublet,which represented mature glycoform of tyrosinase,was in- creased in melanocytes treated with Semen Cuscutae,and in those treated with Semen Persicae.Within those cells,tyrosinase was distributed outside ER resident protein calnexin.Conclusion Both Semen Cus- cutae and Semen Persicae could induce tyrosinase maturation,stability and export from ER to distal site.
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AIM: To study the effect of peach seed (semen Persicae) on gene expression of mouse with sarcoma 180 by means of DNA microarray. METHODS: DNA microarrays were made by spotting PCR products of 4096 mice cDNA noto a specially treated glass slides. The probes were prepared by labeling experimental and control group tumor tissue mRNA with Cy3 dUTP and Cy5 dUTP separately through reverse transcription. The arrays were then hybridized against the cDNA probe mixture and the fluorescent signals were scanned. The data obtained were analyzed from two repeated experiments. RESULTS: 64 genes were obtained by computer processing. CONCLUSION: These genes identified through this approach are considered as potential candidates for PSPA anti tumor effect.
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The amygdalin content in Runing Granules has been determined on the column RP-C_(15), using acetonitrile:water(1:6)as mobile phase and detection wavelength at 240nm.This method showed a high sensitivity,simple operation,good reproducibility and reliable result.It provide a certain base for the quality control of Chinesemateria medica and traditional Chi- nese patent medicine with amygdalin.
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AIM: To discuss the GC analysis of naphtha of Semen Persicae,Flos carthami and its couplet medicine(Semen Persicae plus Flos carthami). METHODS: To determine the relative content of naphtha by vapour distill,Gas Chromatography-Mass Spectrometry and Chemometric Resolution Method and GC unitary area method. RESULTS: The obtained naphtha of Flos carthami consisted of 54 constitutes such as dodecanoic acid and n-Hexadecanoic acid.The obtained naphtha of Semen Persicas comprised 6 constitutes such as benzaldehyde.And the obtained naphtha of Semen Persicae plus Flos carthami possessed 40 coustitutes,such as dodecanoic acid,n-Hexadecanoic acid and benzaldehyde. CONCLUSION: The constitutes Semen Persicae plus Flos carthami are basical the linear addition of constitutes Semen Persicae and Flos carthami but 21 kinds of constitutes in the absence of both Semen Persicae and Flos carthami.