Parámetros productivos del cultivo de cachama blanca Piaractus orinoquensis, en jaulas flotantes / Productive parameters of white cachama culture, Piaractus orinoquensis, in floating cages

RESUMEN La región de la Orinoquia, se caracteriza por poseer espejos de agua que se mantienen durante todo el año, aportando peces para consumo local. La pesca artesanal constituye la principal actividad económica de sustento de comunidades ribereñas, quienes están siendo amenazadas en su seguridad alimentaria, por la disminución del recurso íctico, debido a múltiples causas atribuidas a la actividad humana. Por lo anterior, sistemas de producción en jaulas flotantes, elaboradas con materiales de bajo costo y ubicadas en cursos de aguas naturales, constituyen una alternativa económica para estas comunidades. El objetivo fue evaluar el rendimiento productivo de la cachama blanca en jaulas flotantes, ubicadas en el río Manacacias, manejando tres densidades: 150, 200 y 250 peces/m3. Se sembraron alevinos en jaulas, con dimensiones de 10,2 m³ (3 x 2 x 1,7 m). Los peces fueron alimentados con alimento balanceado, durante 126 días. Al final del experimento, los individuos fueron cosechados. Se produjo una biomasa promedio por densidad de 47,42 kg/m3, en la densidad 150; 45,11 kg/m3, en 200 peces/m3 y 79,65 kg/m3, en la densidad 250. Su conversión alimenticia fue 1,1; 1,32 y 0,8, la sobrevivencia de 83,51, 78,98 y 93,72 %, respectivamente. Se concluye, que la cachama blanca puede ser cultivada en jaulas flotantes a altas densidades, obteniendo parámetros productivos óptimos y, adicionalmente, puede ser una fuente de ingresos significativa para las comunidades.

ABSTRACT The Orinoquia region is characterized by having water bodies that are maintained throughout the year, providing fish for local consumption. Artisanal fishing constitutes the main economic activity for the livelihood of riverine communities, which are being threatened in their food security by the decrease in the fish resource due to multiple causes attributed to human activity. Therefore, production systems in floating cages, made with low-cost materials and located in natural water courses, constitute an economic alternative for these communities. The objective of this work was to evaluate the productive performance of white cachama in floating cages located in the Manacacias river, managing three densities 150, 200 and 250 fish/m3. Fingerlings were sown in cages with dimensions of 10.2 m³ (3 x 2 x 1.7 m). The fish were fed with balanced feed for 126 days. At the end of the experiment the individuals were harvested in three cages for the three densities. An average biomass by density of 47.22 kg/m3 was produced at density 45.11 kg/m3 in 200 fish/m3 and 79.65 kg/m3 at density 250. Its feed conversion was 1.1, 1.32 and 0.8, the survival of 83.51, 78.98 and 93.72 % respectively. It is concluded that cachama blanca can be cultivated in floating cages at high densities, obtaining optimal productive parameters and additionally, it can be a significant source of income for the communities.

Renal myxoboliosis of Metynnis hypsauchen in the Brazilian Amazon: morphological and histopathological aspects

In their natural habitat, fish are constantly threatened by icthyoparasites, notably those from the Phylum Cnidaria, Hatschek, 1888, represented by species of the Myxozoa, responsible for infections in fish that cause complications to their health that can lead to death. Among these parasites, the genus Myxobolus Butschli, 1882 is responsible for the largest number of infections described in fishes from the Americas. This study describes the morphological and histopathological aspects of parasitism by Myxobolus sp. in specimens of Metynnis hypsauchen, obtained from the Capim river, in the municipality of Ipixuna do Pará, Pará, Brazil. During the months of August and March, 2018, 20 animals were captured, euthanized and autopsied. With the aid of a stereomicroscope an external and internal investigation was performed on the animals for the purpose of finding lesions or parasitic cysts, followed by confirmation of infection in Light Microscopy (ML). Cysts and Fragments from parasitized tissues were processed using techniques for histology and Scanning Electron Microscopy (SEM). For histology they were stained with Hematoxylin-Eosin (H-E) and Ziehl-Neelsen and for SEM Micrographs were captured, using equipment from the Museu Paraense Emílio Goeldi. The prevalence of parasitism was 60% (12/20) of the specimens, and the cysts were in the epithelium and lumen of the renal tubules, causing histopathological changes. The characteristics of the parasite spores are those associated with the genus Myxobolus, with an ellipsoid format, two polar capsules and a sporoplasm region. It was possible to confirm a high parasite load of Myxobolus, with compromised renal functions. This study is the first to describe Myxospore in Metynnis hypsauchen.

Population genetic structuring in pacu (Piaractus mesopotamicus) across the Paraná-Paraguay basin: evidence from microsatellites

The Paraná-Paraguay basin encompasses central western Brazil, northeastern Paraguay, eastern Bolivia and northern Argentina. The Pantanal is a flooded plain with marked dry and rainy seasons that, due to its soil characteristics and low declivity, has a great water holding capacity supporting abundant fish fauna. Piaractus mesopotamicus, or pacu, endemic of the Paraná-Paraguay basin, is a migratory species economically important in fisheries and ecologically as a potential seed disperser. In this paper we employ eight microsatellite loci to assess the population structure of 120 pacu sampled inside and outside the Pantanal of Mato Grosso. Our main objective was to test the null hypothesis of panmixia and to verify if there was a different structuring pattern between the Pantanal were there were no physical barriers to fish movement and the heavily impounded Paraná and Paranapanema rivers. All loci had moderate to high levels of polymorphism, the number of alleles varied from three to 18. The average observed heterozygosity varied from 0.068 to 0.911. After the Bonferroni correction three loci remained significant for deviations from Hardy-Weinberg, and for those the frequency of null alleles was estimated. F ST and R ST pairwise comparisons detected low divergence among sampling sites, and differentiation was significant only between Paranapanema and Cuiabá and Paranapanema and Taquari. No correlation between genetic distance and the natural logarithm of the geographic distance was detected. Results indicate that for conservation purposes and for restoration programs small genetic differences detected in the Cuiabá and Paranapanema rivers should be taken in consideration.(AU)

A bacia Paraná-Paraguai compreende o oeste do Brasil, nordeste do Paraguai, leste da Bolívia e o norte da Argentina. O Pantanal do Mato Grosso é uma planície inundada com estações de chuva e seca bem definidas, as características do solo e baixa declividade favorecem a retenção de água proporcionando abrigo para uma abundante ictiofauna. O Piaractus mesopotamicus, ou pacu, endêmico da bacia do Paraná-Paraguai, é uma espécie migratória com importância econômica na pesca e ecológica como potencial dispersor de sementes. Neste estudo utilizamos oito loci de microssatélites para verificar a estrutura populacional de 120 pacus coletados dentro e fora do Pantanal do Mato Grosso. Nosso principal objetivo foi testar a hipótese de panmixia e verificar se haviam diferentes padrões de estruturação entre o Pantanal onde não existem barreiras físicas ao movimento migratório desses peixes em relação aos rios Paraná e Paranapanema com suas inúmeras barragens. Todos os loci apresentaram níveis de polimorfismo de moderado a alto e o número de alelos variou de três a 18. A heterozigosidade média observada variou de 0,068 a 0,911. Depois da correção usando o método de Bonferroni três loci permaneceram estatisticamente significantes para desvios de Hardy-Weinberg, para estes a frequência de alelos nulos foi calculada. Comparações par a par de F ST e R ST detectaram baixa divergência genética entre os locais de coleta e as diferenças foram significantes apenas entre amostras do Paranapanema e Cuiabá e Paranapanema e Taquari. Não foi detectada correlação entre a diversidade genética e o logaritmo natural da distância geográfica. Os resultados indicam que as pequenas diferenças genéticas encontradas nos rios Cuiabá e Paranapanema devem ser levadas em consideração quando se tratar de programas de conservação desta espécie.(AU)

Ocorrência de dois citotipos em Serrasalmus spilopleura Kner, 1858 (characiformes, serrasalmidae) da região de confluência dos Rios Negro e Solimões, Amazonas, Brasil

The karyotype of Serrasalmus spilopleura from the Central Amazon is reported here for the first time. All analized individuals showed 60 chromosomes, as observed in previously published karyotypes of this species from the Paraná-Paraguai basin. However, two different cytotypes were found in sites near the confluence of the Negro and Solimões rivers, where the specimens were collected. Such karyotypic differences were not related to sex differentiation. Moreover, the two amazon cytotypes are different from the three cytotypes described in animals from Paraná-Paraguai basin. The different karyotypes detected so far, in both simpatry and allopatry conditions, suggest that S. spilopleura may be either a cytogenetically heterogeneous species or a complex of sibling species.

Descreve-se pela primeira vez o cariótipo de exemplares de S. spilopleura da Amazônia Central. Todos os indivíduos analisados apresentaram número diplóide igual a 60 cromossomos, em conformidade com os dados da espécie obtidos em indivíduos da bacia do Paraná-Paraguai. No entanto, dois citótipos foram encontrados em simpatria na região de confluência dos rios Negro e Solimões, os quais não estão relacionados a heteromorfismo sexual. Além disso, os dois citótipos da Amazônia diferem dos três anteriormente descritos em exemplares do Paraná-Paraguai. A presença de distintos citótipos encontrados em simpatria e alopatria para S. spilopleura nos leva a sugerir que, ou essa espécie apresenta uma ampla heterogeneidade cariotípica ou existe um complexo de espécies cuja morfologia é muito semelhante.

Ergasilus yumaricussp. n. (COPEPODA: ERGASILIDAE) DAS BRÂNQUIAS DE Pygocentrus nattereri (KNER, 1860), Serrasalmus rhombeus (LINNAEUS, 1819) E Pristobrycon eigenmanni (NORMAN, 1929) (CHARACIFORMES : SERRASALMIDAE) DA AMAZÔNIA BRASILEIRA

Ergasilus yumarikussp. n. (Copepoda, Poecilostomatoida, Ergasilidae) is proposed. The specimens were collected from the gill filaments of the freshwater fishes Pygocentrus nattererì, Serrasalmus rhombeusand Pristobrycon eigenmannifrom Guaporé River near Pimenteiras, Guaporé and Mamoré Rivers, near Surpresa, Jiparaná River near Jiparaná and Pacaás Novos River near Guajará-Mirim, Rondônia state, Brazil. The new species has a falciform pectinate seta on the first exopod that indicates relationship to six previously described Amazonian species. This species differs from others in the shape of body, ornamentation of the legs, antennas and urosomites.

Ergasilus yumaricussp. n. (Copepoda, Poecilostomatoida, Ergasilidae) é proposta. Os espécimens foram coletados dos filamentos branquiais de Pygocentrus nattereri, Serrasalmus rhombeuse Pristobrycon eigenmannido Rio Guaporé próximo à Pimenteiras, rios Guaporé e Mamoré próximo à Surpresa, rio Jiparaná próximo à Jiparaná e Pacaás Novos próximo à Guajará-Mirim, Estado de Rondônia, Brasil. A nova espécie tem uma seta forte, pectinada e falciforme no primeiro exopodito, indicando uma relação com outras seis espécies amazônicas. Esta espécie difere das outras na forma do corpo, ornamentações das pernas, antenas e urossomitos.