ABSTRACT
A review is presented on different analytical techniques used for qualitative and quantitative analysis of serratiopeptidase, a proteolytic enzyme, which has recently gained importance as an anti-inflammatory agent. Efforts have been made to collate all the relevant references to the extent possible. The review discusses the advantages and disadvantages of the cited analytical techniques, which will help to give insights into the methods used for estimation of serratiopeptidase as such, from clinical isolates and from its dosage forms. The review highlights the basic as well as advanced techniques performed for estimating serratiopeptidase. The techniques illustrated here have been demonstrated to be useful for qualitative and quantitative determination of serratiopeptidase and may find application in analyzing other related proteases.
ABSTRACT
Enzymes control all metabolic processes in human system from simple digestion of food to highly complex immune response. Physiological reactions occuring in healthy individuals are disturbed when enzymes are deficient or absent. Enzymes are administered for normalizing biological function in certain pathologies. Initially, crude proteolytic enzymes were used for the treatment of gastrointestinal disorders. Recent advances have enabled enzyme therapy as a promising tool in the treatment of cardiovascular, oncological and hereditary diseases. Now, a spectrum of other diseases are also covered under enzyme therapy. But, the available information on the use of enzymes as therapeutic agents for different diseases is scanty. This review details the enzymes which have been used to treat various diseases/disorders.
ABSTRACT
In recent years, albumin nanoparticles have been widely studied for delivery of various active pharmaceuticals with enhanced accumulation at the site of inflammation. Albumin is a versatile carrier to prepare nanoparticles and nanospheres due to its easy availability in pure form, biodegradability non-toxic and non-immunogenic nature. The mechanism of action of Serratiopeptidase appears to be hydrolysis of histamine, bradykinin and serotonin. Serratiopeptidase also has a proteolytic and fibrinolytic effect. Protein i.e. bovine serum albumin was used to entrap serratiopeptidase enzyme. Protease activity of the enzyme was checked and method was validated to access the active enzyme concentration during formulation. Solvent desolvation method was used for the preparation of BSA nanoparticles. Effect of buffer pH was checked on the enzyme activity. Chloroform was selected and used as solvent for nanoparticle preparation. Effect of various variables such as concentration of BSA, agitation rate, glutarldehyde concentration, time of crosslinking etc. on the formulation was studied. Formed nanoparticles were characterized for drug content, in-vitro release, entrapment efficiency, particle size and size distribution. The formed serratiopeptidase loaded albumin nanoparticles may be used for the treatment of arthritis.
ABSTRACT
Introduction: Surgical extraction of lower 3 rd molar is the most frequent intervention in oral surgery. This procedure is often associated with significant post operative swelling that may have both biological and social implications. Various studies have been done using different anti inflammatory drugs to study their impact on inflammation. Material and Methods: This study was conducted on 110 patients who had undergone surgical removal of mandibular third molar. The subjects were divided into two groups by double blind method. In addition to post operative swelling and pain , trismus was evaluated using two different groups of drugs. Results: One group was administered 1 mg of dexamethazone every 8 hours for 3 days post operatively. The other group was administered 10 mg of serratiopaptidase every 8 hours for 3 days post operatively. Swelling, pain and trismus were assessed on the 1 st , 3 rd , 5 th and 7 th post operative days. The results of the studies were statistically analysed. Conclusion: The results showed dexamethazone was more effective in reduction of swelling and pain in comparison with serratiopaptidase. Both dexamethazone and serratiopaptidase had the same effect on trismus.