Design, synthesis, in silico studies, and antidiabetic activity of several sulfanilamide incorporated 2,3-disubstitut-ed thiazolidin-4-ones

A panel of 2,3-disubstituted thiazolidin-4-ones 4a-n was synthesised from Schiff bases 3a-n derived from sulfanilamide, by reaction with thioglycolic acid. The compounds were characterised by means of IR, NMR, and Mass spectral data. Compounds 4a-n were screened for DPPH scavenging assay and compounds 4e, 4h, 4i, and 4n exhibited moderate activity. Compounds 4e, 4h, and 4i were tested at 200 mg/kg and 4e at 50 mg/kg b.w. orally for antidiabetic activity in fructose induced diabetic rats. They exhibited significant antidiabetic activity compared to the control group. Pioglitazone was used as a standard drug. The tested compounds exhibited better and ignificant serum cholesterol lowering activity when compared with the control and standard groups. They also reduced the triglyceride level after the 21st day; however, it was insignificant when compared to the control group. Compound 4n displayed the highest binding energy when docked with PPAR-γ followed by compounds 4e, 4h, and 4i when compared to pioglitazone. The physicochemical, drug likeness and ADME properties of the title compounds were found to be satisfactory.

Se sintetizó un panel de tiazolidinas-4-onas 2,3-disustituidas 4a-n a partir de las bases de Schiff 3a-n derivadas de la sulfanilamida por reacción con ácido tioglicólico. Los compuestos se caracterizaron por IR, RMN y datos espectrales de masa. Los compuestos 4a-n se analizaron para DPPH y los compuestos 4e, 4h, 4i y 4n mostraron una actividad moderada. Los compuestos 4e, 4h y 4i se probaron a 200 mg/kg y 4e a 50 mg/kg b.w. oralmente para la actividad antidiabética en ratas diabéticas, inducida por fructosa. Los compuestos mostraron una actividad antidiabética muy significativa en comparación con el grupo control. La pioglitazona se utilizó como fármaco estándar. Los compuestos ensayados mostraron una mejor y significativa actividad reductora del colesterol sérico en comparación con los grupos control y estándar. Estos compuestos también redujeron el nivel de triglicéridos después del 21° día, aunque fue insignificante en comparación con el grupo control. El compuesto 4n mostró la mayor afinidad de unión cuando se acopló a PPAR-γ, seguido de 4e, 4h y 4i en comparación con la pioglitazona. Las propiedades fisicoquímicas, la similitud con el fármaco y las propiedades ADME de los compuestos fueron satisfactorias, lo que los convierte en útiles agentes antidiabéticos.

Um painel de 2,3-disubstituído thiazolidina-4-ones 4a-n foram sintetizados a partir de bases Schiff 3a-n derivado da sulfanilamida por reacção com ácido tioglicólico. Os compostos eram caracterizado por IR, NMR e dados espectrais de massa. Os compostos 4a-n foram rastreados para O ensaio DPPH de limpeza radical e os compostos 4e, 4h, 4i e 4n exibiram actividade moderada. Os compostos 4e, 4h e 4i foram testados a 200 mg/kg e 4e a 50 mg/kg de peso corporal por via oral para antidiabéticos. actividade em ratos diabéticos induzidos por frutose. Exibiram uma actividade antidiabética altamente significativa actividade em comparação com o controlo. A pioglitazona foi utilizada como droga padrão. Os compostos testados exibiu uma melhor e significativa actividade de redução do colesterol sérico quando comparado comde triglicéridos após o 21° dia; no entanto, foi insignificante quando comparado com o controlo. O composto 4n mostrou a maior afinidade de ligação quando acoplado com PPAR-γ seguido de 4e, 4h, 4i quando comparado com pioglitazona. O propriedades físico-químicas, de semelhança com drogas e ADME dos compostos do título de propriedade também foram encontrados paraser satisfatórios, tornando-os agentes antidiabéticos úteis.

Can seizures cause glycemic disorder in children with epilepsy? a cross-sectional study

Background: Aim of this study is to investigate the serum glucose level in children with epilepsy.Methods: A cross-sectional study was conducted at Pediatric Centre of Hue Central Hospital between June 2017 and June 2019. A serum glucose level test was performed after the seizure.Results: In total, 144 patients were included in the study. Of these, the most age group was 0 - <5 years (accounted for 59.7%), followed by group 5-10 years (24.3%) and 11-15 years (16%). Female was more likely than male (52.8% vs 47.2%). The most common type was generalized epilepsy (accounted for 72.2%), and the least was un-classified epilepsy (6.3%). The blood test investigated 19 (13.2%) of patients with hyperglycemia and 1 (1.4%) with hypoglycemia.Conclusions: The present study highlights that seizure can cause glycemic disorder in children with epilepsy.

Association between fasting glucose and branchial-ankle pulse wave velocity among hypertensive population in rural areas of Eastern China / 中华疾病控制杂志

Objective To explore the relationship between fasting blood glucose and branchial-ankle pulse wave velocity (baPWV) levels among hypertensive population in rural areas of Eastern China. Methods A cross-sectional analysis were conducted among hypertensive population in Lianyungang and Anqing city from July to September 2013. Multivariable linear regression models were used to analyze the association of serum glucose and baPWV. Results A total of 3 894 hypertensive participants were included in the present study. The prevalence of normal fasting glucose (NFG), impaired fasting glucose (IFG) and diagnosed diabetes (DM) were 44.5%, 43.0%, and 12.5%, respectively. Each 1-mmol/L increase in fasting blood glucose levels was significantly associated with a 26.2 cm/s increase in baPWV levels (95% CI: 19.6-32.8, P<0.001). Consistently, significantly higher baPWV levels were observed in participants with IFG (β=50.4 cm/s, 95% CI: 28.4-72.3, P<0.001) and DM (β=113.4 cm/s, 95% CI: 80.2-146.5, P<0.001) when compared to those with NFG. Furthermore, a stronger positive association between fasting blood glucose and baPWV was observed in participants with systolic blood pressure (SBP) ≥140 mmHg (β=34.3 cm/s, 95% CI: 24.5-44.2, P<0.001; vs.<140 mmHg; β=18.1 cm/s, 95% CI: 9.1-27.2, P<0.001; Pinteraction=0.027). Conclusion There was a positive association between fasting blood glucose and baPWV levels among hypertensive population in rural areas of Eastern China.

A Potential Issue with Screening Prediabetes or Diabetes Using Serum Glucose: A Delay in Diagnosis

The aim of this study was to compare the fasting serum glucose level with the fasting plasma glucose level for diagnosing hyperglycemic states in real-life clinical situations. Additionally, we investigated a usual delay in sample processing and how such delays can impact the diagnosis of hyperglycemic states. Among 1,254 participants who had normoglycemia or impaired fasting glucose (IFG) assessed by the fasting serum glucose level, 20.9% were newly diagnosed with diabetes based on the plasma fasting glucose level. Of the participants with normoglycemia, 62.1% and 14.2% were newly diagnosed with IFG and diabetes, respectively, according to the plasma fasting glucose level. In our clinical laboratory for performing health examinations, the time delay from blood sampling to glycemic testing averaged 78±52 minutes. These findings show that the ordinary time delay for sample processing of the serum glucose for screening hyperglycemic states may be an important reason for these diagnoses to be underestimated in Korea.

Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure

BACKGROUND: In recent times, there has been an increase in the incidence of type 2 diabetes mellitus (T2DM) particularly in children. Adipocyte dysfunction provide a critical link between obesity and insulin resistance resulting in diabetes outcome. Further, environmental chemical exposure during early years of life might be a significant contributing factor to the increase in the incidence of T2DM. This study tests the idea that exposure to environmental contaminants (2-aminoanthracene [2AA]) in utero will show effects in the adipose tissue (AT) that signify T2DM vulnerability. 2AA is a polycyclic aromatic hydrocarbon found in a variety of products. METHODS: To accomplish the study objective, pregnant dams were fed various amounts of 2AA adulterated diets from gestation through postnatal period. The neonates and older offspring were analyzed for diabetic-like genes in the ATs and analysis of serum glucose. Furthermore, weight monitoring, histopathology and immunohistochemical (IHC) staining for CD68 in AT, adipocyte size determination and adiponectin amounts in serum were undertaken. RESULTS: Up-regulation of adiponectin and interleukin-6 genes were noted in the pups and older rats. Combination of intrauterine 2AA toxicity with moderate high fat diet exhibited gene expression patterns similar to those of the neonates. Elevated serum glucose levels were noted in treated groups. IHC of the AT indicated no significant malformations; however, CD68+ cells were greater in the animals treated to 2AA. Similarly, mean sizes of the adipocytes were larger in treated and combined 2AA and moderate high fat animals. Adiponectin was reduced in 2AA groups. CONCLUSION: From the preceding, it appears intrauterine 2AA disturbance, when combined with excess fat accumulation will lead to greater risk for the diabetic condition.

Significance of cerebrospinal fluid lactate level in diagnosing neonatal bacterial meningitis / 中华实用儿科临床杂志

Objective To study the significance of the cerebrospinal fluid(CSF)lactate level in diagnosing neonatal bacterial meningitis(BM). Methods The CSF samples were collected from neonates admitted to Neonatal Ward of Children's Hospital of Fudan University between January 2014 and March 2015. CSF lactate and glucose con-centrations were measured with blood - gas analyzer. CSF and serum glucose levels were measured with glucometer. The enrolled neonates were divided into 2 groups based on CSF culture,CSF white blood cells(WBCs)and clinical presenta-tion:observation group(neonates with BM)and control group(neonates without BM). Statistical analysis of data was performed with Stata 12. 0. Results A total of 93 infants(16 assigned to observation group and 77 assigned to control group)met the inclusion criteria. Neonates in observation group had higher median CSF lactate level(4. 2 mmol/ L)and CSF lactate/ glucose ratio(L/ Gcsf)(2. 32 mmol/ L),than those in control group(1. 3 mmol/ L,0. 52),and there were significant differences(Z = - 6. 19,- 5. 92,all P ﹤ 0. 05). CSF glucose levels were lower in observation group(me-dian,1. 25 mmol/ L)than those in control group(median,2. 5 mmol/ L),and the difference was significant(Z = 4. 97, P ﹤ 0. 05);CSF/ serum glucose ratio(CSF/ Sglu)were lower in observation group(median,0. 44 vs 0. 81 in control group),and the difference was significant(Z = 4. 43,P ﹤ 0. 05). The optimal CSF lactate cutoff point of 2. 2 mmol/ L had a positive predictive value(PPV)of 72. 7% and negative predictive value(NPV)of 100. 0% for bacterial meningi-tis. The optimal L/ Gcsf cutoff point of 1. 24 had a PPV of 94. 1% and NPV of 100. 0% . The optimal CSF glucose cutoff point of 2. 0 mmol/ L had a PPV of 65. 0% and NPV of 95. 9% . The optimal CSF/ Sglu cutoff point of 0. 6 had a PPV of 60. 0% and a NPV of 96. 9% . Conclusion CSF lactate may be an excellent biomarker for early diagnosis of neo-natal BM.

Study about relationship between VitD3 and insulin level in patients with 3-5 stage chronic kidney disease / 中国免疫学杂志

Objective:To investigate the relationship between VitD 3 concentration and glucose and insulin levels of OGTT in patients with CKD 3-5 stages.Methods: We included the patients with CKD 3 and 4 and 5 stages who fulfill the including standard.All patients were recorded the concentrations of [1,25 (OH):D3]concentration of glucose and insulin at fasting ,postprandial 1 h,2 h during OGTT and concentration of glycosylated hemoglobin level ,C peptide concentration.We performed the correlation analysis about [1,25 (OH):D3],glucose and insulin.Results: We totally included 91 patients with 3-5 stages CKD into our study.The D3 concentration of stage 3 were 160.9-261.3 mmol/L[(218.38±8.67)mmol/L] of stage 3,75.2-166.3 mmol/L[(117.01±4.72) mmol/L] of stage 4 and 11.8-96.5 mmol/L[(41.91±12.83)mmol/L] of stage 5 (P<0.05).The average concentrations of serum glucose at fasting,1 h after the meal and 2 h after the meal was(4.74±0.21)mmol/L,(8.31±0.43)mmol/L and(7.36±0.32)mmol/L in 3 stage and (4.92±0.25) mmol/L,(9.14±0.15) mmol/L and (9.14±0.39)mmol/L at 4 stage and (4.81±0.13)mmol/L, (10.72±0.41)mmol/L and (10.72±0.49)mmol/L at 5 stage (P<0.05).The average concentrations of insulin during OGTT at fasting,1 h after the meal and 2 h after the meal was (6.58±0.32) μU/L,(57.78±5.63)U/L and (42.77±8.45)U/L in 3 stage (6.03±0.53)U/L,(55.69±7.35)U/L and (62.52±5.39)U/L in 4 stage and (6.12±0.65)U/L,(62.82±9.73)U/L and (77.34± 8.62)U/L in 5 stage (P<0.05).Correlation analysis shows that the concentration of 1,25 (OH):D3 of different stages of patients with CKD and vitamin D 3 concentration and glucose tolerance test was found to be inversely associated with the insulin levels ( P<0.05 ) . Conclusion:There are obvious differences of concentration of vitamin D 3 between patients with 3-5 stages of chronic kidney disease (CKD).There also showed a negative correlation relationships between glucose and insulin levels ,and vitamin D3 concentration and glucose and insulin levels at OGTT of patients with 3-5 stages CKD.

Self-rated Subjective Health Status Is Strongly Associated with Sociodemographic Factors, Lifestyle, Nutrient Intakes, and Biochemical Indices, but Not Smoking Status: KNHANES 2007-2012

Despite advertised health warnings regarding the deadly hazards of smoking, many people have not heeded recommendations to quit smoking. We examined factors that affect self-rated subjective health status (SRH) scores among lifestyle, nutrient intake and biochemical parameters, and the association of SRH scores and smoking status in a large Korean adult population. Adjusted odd ratios for SRH were calculated for smoking status, selected biochemical data, and food and nutrient intake obtained using the 24-hr recall method after covariate adjustment in the 2007-2012 Korean National Health and Nutrition Examination Survey (27,534 men and women aged > or = 20 yr). Age, sex, income, education, drinking, exercise and stress levels were associated with SRH scores, regardless of smoking status (P < 0.001). Interestingly, people in any smoking status groups considered the well-known indicators for metabolic diseases (HDL cholesterol, glucose, aspartate aminotransferase, and alanine aminotransferase in the circulation), and the intake of fiber, total vitamins A, and vitamin C as indicators of SRH. Especially in current smokers, higher intake of nutritious food groups such as grains (OR = 1.227), vegetables (OR = 1.944), and milk (OR = 2.26) significantly increased the adjusted odds ratio of SRH. However, smoking status was not associated with SRH scores. In conclusion, SRH is affected by the indices related to health but not smoking status in Korean adults. The development of a new indicator of the direct adverse effects of smoking at regular health check-ups might be required to modulate the SRH in smokers and a nutritional education should not include the possible attenuation of adverse effects of smoking by good nutrition.

Veracidad de un analizador de gasometria para determinar glucemia, respecto a un metodo de laboratorio convencional / Reliability of a gas analyzer to determine glycemia compared to the conventional laboratory method

Objetivos:Determinar el grado de veracidad en los resultados de glucosa, medidos en un equipo de gasometría, mediante lacomparación con un procedimiento de uso habitual en el laboratorio, siguiendo el procedimiento indicado en la guía EP9–A2 delClinical and Laboratory Standards Institute(CLSI).Diseño:Estudio descriptivo con muestreo no probabilístico.Institución:HospitalEdgardo Rebagliati Martins, EsSalud, Lima, Perú.Material:Muestra sanguínea de 234 sujetos provenientes de los servicios deemergencia y la unidad de cuidados intensivos.Métodos:Se procesó glucemia en los equipos ADVIA1800 y el gasómetro ABL800.Se comparó los resultados de ambos analizadores siguiendo las directrices de la mencionada guía, además del análisis gráfico deBland-Altman y el cálculo del coeficiente de concordancia correlación (CCC) de Lin.Principales medidas de resultados:Concentraciónde glucosa sérica.Resultados:La media de glucemia obtenida fue 1,6 mg/dL mayor para ABL800 que para el ADVIA1800. Los dosmétodos de medida seguían una relación lineal, obteniéndose un coeficiente de correlación de 0,9995, con un intervalo de confianza(IC) al 95% de 0,9994a 0,9996. Los resultados de glucosa del método de estudio fueron aceptables según los requerimientos decalidad, lo cual se confirmó con los análisis estadísticos de Bland-Altman y el valor del CCCL de 0,9995, con un IC de 95% de 0,9993a 0,9996.Conclusiones:El analizador ABL800 resultó adecuado para la monitorización de glucemia; presentó una buena asociaciónlineal y veraz, cuando fue comparado con el método de referencia del laboratorio.

Objectives: To determine the glucose reliability results measured in a gas equipment as compared with a reference method commonly used in the laboratory. The Clinical and Laboratory Standards Institute (CLSI) guide EP9- A2 instructions were followed. Design: Descriptive study with non-probability sampling. Setting: Hospital Edgardo Rebagliati Martins, EsSalud, Lima, Peru. Materials: Blood sample of 234 subjects from the emergency services and intensive care unit. Methods: Blood glucose was processed with the ADVIA1800 equipment and the ABL800 gasometer. Results of both analyzers were compared following the mentioned guide directives, the Bland-Altman plot analysis and the Lin’s concordance correlation coefficient (CCC) calculation. Main outcome measures: Serum glucose concentration. Results: Average blood glucose levels obtained were 1.6 mg/dL higher for ABL800 than for ADVIA1800. Both methods showed a high positive correlation (beta coefficient 0.9995 and 95 per cent, 95 per cent CI 0.9994 to 0.9996). Glucose results for the method studied were acceptable, as confirmed with the Bland-Altman statistical analysis (0.9995 CCC value, 95 per cent CI 0.9993 to 0.9996). Conclusions: The ABL800 analyzer is suitable for blood glucose monitoring, presenting an excellent correlation with the reference laboratory method.

Serum Lipid Profile, Serum Magnesium and Fasting Serum Glucose in Newly Diagnosed Type 2 Diabetic Subjects.

Type 2 diabetes is considered as a major health burden due to its rising prevalence and disabling, life threatening complications. Dyslipidemia, often coexisting with T2DM as a feature of insulin resistance, is hypothesized to be linked with altered magnesium homeostasis. This study was designed to evaluate the serum magnesium levels and its influence on serum lipids in type 2 diabetics. Lipid profile, serum magnesium (Mg) and fasting serum glucose (FSG) were measured in 30 newly diagnosed normotensive type 2 diabetic patients chosen as cases (Group II) just before introducing any treatment, and was compared with that of 30 healthy controls (Group I). The serum magnesium was found to be significantly lower (p<0.001) and LDL-c was found to be significantly higher (p<0.01) in cases. The correlation analysis revealed a significant negative association of FSG to serum magnesium (r= -0.720), total cholesterol (r=-0.483) and a positive correlation to HDL-c (r=-0.440). However, serum magnesium showed a significant positive relation only with serum HDL-c (r =0.372, p<0.05). Serum magnesium and lipid fractions showed wide range of variation within the normal reference ranges in the newly diagnosed T2D subjects. Further large scale studies are needed to elucidate the association of serum magnesium with lipid profile changes. Estimation of serum magnesium level may prove useful in T2DM with normal or abnormal lipid levels or in those who are prone to develop dyslipidemia or certain complications associated with dyslipidemia. Key words: Lipid Profile, Type

Estimation of salivary glucose level and plasma glucose level in subjects with and without diabetes mellitus: a comparative study.

Objective: The objective of this study was to estimate and correlate salivary glucose levels and plasma glucose levels in non-diabetic subjects, controlled and uncontrolled diabetic subjects and to assess if salivary glucose can be a potentially useful non-invasive tool in diagnosing diabetes mellitus and in monitoring of glycemic control in diabetic patients. Materials and methods: A total of 90 subjects aged between 40-60 years participated in the study. Diabetic status was determined by estimation of random non-fasting plasma glucose levels and Glycosylated haemoglobin levels. Both unstimulated and stimulated saliva were collected and investigated for glucose levels. Salivary glucose levels were measured using the glucose oxidase method. Results: Salivary glucose levels were significantly higher in diabetics than non-diabetics. Mean un-stimulated salivary glucose level was 1.15 mg/dL in control group, 2.04 mg/dL in controlled diabetic group and 3.99 mg/dL in un-controlled diabetic group. There was a significant positive correlation between salivary and plasma glucose levels. Conclusion: These results show that salivary glucose concentration can be used as a potentially useful non-invasive tool for diagnosing diabetes mellitus and monitoring glycemic control in diabetic patients.

Mechanism and management of perioperative serum glucose abnormality for major hepatobilipancreatic surgery / 中华临床营养杂志

Serum glucose control is an important aspect of perioperative management. For major hepatobilipancreatic surgery, specific pathophysiologic changes can lead to specific problems. This article reviews recent advances in the perioperative serum glucose control for major hepatobilipancreatic surgery.

Effects of carbohydrate-electrolyte solution on serum glucose, pancreas islet function, and safety in elderly patients after abdominal operation / 中华临床营养杂志

Objective To evaluate the effects of carbohydrate-electrolyte solution(CES)on serum glucose,pancreas islet function,and safety in elderly patients after abdominal operation.Methods In this prospective,double-blinded,randomized,and controlled study,40 elderly patients who met the defined criteria were enrolled.Subjects in CES group were intravenously administered with 1 000 ml CES for consecutive three days beginning from the 1st and 2nd post-operative day,while subjects in the control group were administered with 10% glucose of the same volume under the same arrangement.The changes of serum glucose,insulin and insulin C-peptide,as well as lactic acid and uric acid and uric acid were determined before and after injection.Adverse events were recorded.Results All patients completed the study.The increase rate of serum glucose was significantly lower on the 2nd and 3rd day after injection in CES group than in control group(P=0.008,P:0.001).Blood insulin and insulin C-peptide levels showed increasing trends in both two groups,but were not significantly different between two groups(P=0.612,P=0.213).In the CES group,6 patients experienced systemic inflammatory response syndrome and 4 patients had infective complications after surgeries ;on the contrary,these two numerals were 8 and 6 in the control group(P=0.639,P=0.606).No increase in serum lactic acid or uric acid was detected.Conclusion Appropriate application of CES has minimal effect on the blood gluocse and pancreas islet function in elderly patients after abdominal surgery and may be helpful to improve clinical outcomes.

Effect of Dehulling and Cooking of Lentils (Lens Culinaris, L.) on Serum Glucose and Lipoprotein Levels in Streptozotocin-Induced Diabetic Rats

This study was conducted to investigate the effect of lentils on serum glucose and serum lipid levels in diabetic rats. Forty adult male Sprague-Dawley rats, 12 weeks of age weighing 220-290g, were used. Diabetes was induced by streptozotocin at a level of 35 mg/kg intra-peritoneally. The animals were randomly divided into five groups, eight animals each: a casein diet (control), raw whole lentil (RWL), cooked whole lentil (CWL), raw dehulled lentil (RDL) and cooked dehulled lentil (CDL). Animals were fed with experimental diets for six weeks, sacrificed and blood samples were taken. Serum glucose level of the CDL group (387.9 ± 53.3 mg/dl) was significantly lower (P0.05) were detected in triglycerides, total cholesterol and LDL cholesterol among the experimental groups. It is concluded that cooked lentils rather than raw lentils was more effective in lowering blood glucose and improving HDL cholesterol in diabetic rats. There was no difference between whole and dehulled lentils with regard to effects on blood glucose and HDL cholesterol levels.

Joint Optimization of Savitzky-Golay Smoothing Models and Partial Least Squares Factors for Near-infrared Spectroscopic Analysis of Serum Glucose / 分析化学

The optimal model for the near-infrared spectroscopic analysis of serum glucose was established by partial least squares(PLS) and Savitzky-Golay(SG) smoothing method. Based on the prediction effect of the optimal single wave number model, a new dividing method for calibration set and prediction set was given. The calibration and prediction models were established by PLS method adopting the combination bands of 10000-5300 cm~(-1) and 4920-4160 cm~(-1) with Savitizky-Golay(SG) smoothing. By extending the number of smoothing points to 5, 7, ..., 87(odd) and polynomial degree to 2, 3, 4, 5, 6, fourteen smooth coefficient tables including 582 smooth modes were calculated. All PLS models corresponding to all smooth modes and all PLS factors(1-40) were constructed. The optimal model was selected by the prediction effect. And the derivation order was 1, the polynomial degree was 3 or 4, the number of smoothing points was 53, the optimal factor was 7 and the optimal RMSEP reach 0.376 mmol/L. The dividing method for calibration set and prediction set, the extending of SG smoothing modes, large-scale optimization combining SG smoothing modes and PLS factors can be effectively applied for the model optimization of near-infrared spectroscopic analysis.

Application of detection of C-reactive protein, serum glucose concentration and APACHE Ⅱ grade in systemic inflammatory response syndrome / 中国综合临床

Objective To study the value of detection of C- reactive protein(CRP), serum glucose concen-tration and APACHE Ⅱ grade for estimating inflammation, pathogenetic condition and prognosis of systemic inflam-matory response syndrome (SIRS) patients.Methods 50 SIRS patients in ICU were selected and were divided into S1 and S2 subgroup according to SIRS diagnosis standard, and 18 patients developed MOBS.30 patients without SIRS, were selected as control group.Detection of CRP, serum glucose concentration and APACHE Ⅱ grade were per-formed.Results CRP(76.19±11.98 mg/L vs.14.04±5.70 mg/L) ,serum glucose concentration(7.10±1.30 mmol/L vs.5.32±1.09 mmol/L) and APACHE Ⅱ grade (20.06±6.39 vs.7.90±3.54) of SIRS group were higher than that of non-SIRs group (P<0.01).CRP, serum glucose concentration and APACHE Ⅱ grade all are higher in group S2 than that of group S1 (91.25±9.89 mg/L vs.62.29±10.38 mg/L,8.32±2.40 mmol/L vs.7.10±1.69 mmol/L,26.72±5.14 vs.18.37±5.70).MOBS incidence and case fatality in S2 subgroup (12/15 and 6/35) were higher than that of S1 subgroup(10/15 and 3/35 ) (P<0.01 ).Conclusion CRP, serum glucose concentration and APACHE Ⅱ grade are increased in SIRS patients and with their increasing, MOBS incidence and case fatality were also increasing.CRP, serum glucose concentration and APACHE Ⅱ grade are significant factors in estimating SIRS patients pathogenetic condition and their prognosis.

Effects of Soy Isoflavone on Serum Glucose and Lipid in Orchiectomized Rats / 环境与健康杂志

Objective To investigate the effects of environmental endocrine disruptors-phytoestrogen soybean isoflavone on serum glucose and lipid in rats with orchiectomy.Methods Fifty-four healthy clean male Wistar rats aged 7 weeks were randomly divided into 6 groups,9 in each group,according to their weight and named as the groups of SHAM,ORCH,L-SI,M-SI,H-SI and EC.The rats were injected intraperitoneally with 1.5% sodium pentobarbital according to 40 mg/kg,and they were either shamoperated or orchiectomized.The rats were fed on high fat diet for 4 weeks.There was 1,3,9 g soybean isoflavone and 0.45 mg estrobene per kilogram high fat diet in L-SI,M-SI,H-SI and EC respectively.At the 2nd and 4th week,serum insulin,fasting blood glucose and lipid (TG,TC,HDL and LDL) were detected.Results The weights of rats with orchiectomy was significantly decreased compared with sham-operated rats,and compared with the EC group,the weights of other groups'rats were significantly higher.The weight was decreased along with the increase of the dose of soy isoflavone.At the end of 4 weeks,SIF significantly decreased serum glucose compared with ORCH,and the insulin level was significantly increased in M-SI and H-SI group.SIF and estrogen could significantly decrease TC,but they did not have obvious effect on LDL.SIF could not improve HDL level in rats with orchiectomy.Even though there was no statistic difference,the TG levels of SIF groups were deceased.Conclusion SIF can decrease serum glucose level via raising insulin level in rats with orchiectomy.SIF can decrease serum TC level significantly and regulate TG level while there is no influence on HDL and LDL.

Research into the influence of diet structure and physical activity on the lipid level / 中国实用内科杂志

Objective To evaluate the influence of diet structure and physical activity on the lipid level in different area of China.Methods A total of 145 people were selected by simple random sampling,including 52 residents,45 urban residents of Panzhihua and 48 rural residents of Panzhihua,each of them undergoing the laboratory examination and dietary and physical activity survey.Food frequency questionnaire and physical activity questionnaire were used.Results The blood sugar,TC and LDL-C level in Shanghai residents were higher than those of urban and rural residents of Panzhihua(P0.05)while the energy exhaust of Shanghai residents was higher than those of urban and rural residents of Panzhihua(P

Significance of Postprandial Glucose Test for the Diagnosis of Type 2 Diabetes / 中国慢性病预防与控制

Objective To examined the relationship between the Fast Plasma Glucose(FPG) and the Postprandial Plasma Glucose(PPG) by analyzing 3 588 OGTT and IRT,and assessed the role of PPG in the diagnosis of type 2 diabetes.Methods Data of OGTT and IRT in 3 588 patients from 2000-Jan to 2007-June were collected and the distribution of the patients evaluated with cut-points of fasting plasma glucose by 5.6 mmol/L,6.1 mmol/L & 7.0 mmol/L and postprandial plasma glucose by 7.8 mmol/L and 11.1mmol/L were analyzed.T-test and corrected t-test of two independent samples have been done with SPSS 11.5.Results By WHO standard,3 097 T2DM were diagnosed.Of 2654 T2DM patients diagnosed by FPG≥7.0 mmol/L,54 patients showed 2 h PG0.05).202 cases were NGT(but hyperinsulinemia and/or delayed insulin secretion were found in 113 of them,55.94%).Other 289 cases were IFG and/or IGT.In the 291 cases diagnozed with 1 h PG≥11.1 mmol/L and 2 h PG

Association between Serotonin Transporter Polymorphism and the Effect of Atypical Antipsychotics on Serum Glucose and Lipids / 대한정신약물학회지

OBJECTIVE: The author investigated the association between the genotype distribution of 5-HTTLPR and the effects of atypical antipsychotics on serum glucose and lipids in schizophrenic patients. METHODS: Study subjects were 66 schizophrenic patients taking atypical antipsychotics (risperidone, olanzapine, clozapine, quetiapine, zotepine). The control group consisted of 82 schizophrenic patients taking typical antipsychotics (haloperidol). All subjects were medicated for at least 12 weeks. The author examined serum fasting blood sugar (FBS), HbA1c, total cholesterol, triglyceride, and the genotype distribution of 5-HTTLPR in all subjects. The presence of the 5-HTTLPR gene was determined by using polymerase chain reaction of genomic DNA with primers flanking the promoter regions of the 5-HTT gene. Between group comparisons of the genotype distribution and the effects of antipsychotics on the serum glucose and lipid levels were performed by using score test for t-test, one way ANOVA, and chi-square test. RESULTS: There was a significant increase in FBS level in all patients taking atypical antipsychotics except for those treated with risperidone. However, there was no statistically significant correlation between 5-HTTLPR genotype distribution and the effect of atypical antipsychotics on FBS, HbA1c, total cholesterol, and triglyceride serum levels in schizophrenic patients. CONCLUSION: These results suggest that 5-HTTLPR polymorphism has no significant association with the effect of atypical antipsychotics on serum glucose and lipids in Korean schizophrenic patients.